Glutathione in Intact Vacuoles: Comparison of Glutathione Pools in Isolated Vacuoles, Plastids, and Mitochondria from Roots of Red Beet

Pradedova, E. V.; Nimaeva, O. D.; Karpova, A. B.; Семенова, Н. В.; Ракевич, А. Л.; Nurminskii, V. N.; Степанов, А. В.; Salyaev, R. K.

doi:10.1134/s1021443718020048

Cited by 6 publications

(2 citation statements)

References 24 publications

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“…However, the non-enzymatic way of utilizing radicals in vacuoles, which includes low-molecular species like flavonoids and antioxidants, seems to be the most important. Vacuoles contain approximately 2 mM of ascorbate ( Zechmann, 2018 ) and approximately 100 μM of glutathione ( Pradedova et al, 2018 ).…”

Section: Survey Methodologymentioning

confidence: 99%

Subcellular compartmentalization of the plant antioxidant system: an integrated overview

Bobrovskikh

Зубаирова

Kolodkin

et al. 2020

PeerJ

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The antioxidant system (AOS) maintains the optimal concentration of reactive oxygen species (ROS) in a cell and protects it against oxidative stress. In plants, the AOS consists of seven main classes of antioxidant enzymes, low-molecular antioxidants (e.g., ascorbate, glutathione, and their oxidized forms) and thioredoxin/glutaredoxin systems which can serve as reducing agents for antioxidant enzymes. The number of genes encoding AOS enzymes varies between classes, and same class enzymes encoded by different gene copies may have different subcellular localizations, functional loads and modes of evolution. These facts hereafter reinforce the complex nature of AOS regulation and functioning. Further studies can describe new trends in the behavior and functioning of systems components, and provide new fundamental knowledge about systems regulation. The system is revealed to have a lot of interactions and interplay pathways between its components at the subcellular level (antioxidants, enzymes, ROS level, and hormonal and transcriptional regulation). These facts should be taken into account in further studies during the AOS modeling by describing the main pathways of generating and utilizing ROS, as well as the associated signaling processes and regulation of the system on cellular and organelle levels, which is a complicated and ambitious task. Another objective for studying the phenomenon of the AOS is related to the influence of cell dynamics and circadian rhythms on it. Therefore, the AOS requires an integrated and multi-level approach to study. We focused this review on the existing scientific background and experimental data used for the systems biology research of the plant AOS.

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Section: Survey Methodologymentioning

confidence: 99%

Subcellular compartmentalization of the plant antioxidant system: an integrated overview

Bobrovskikh

Зубаирова

Kolodkin

et al. 2020

PeerJ

View full text Add to dashboard Cite

show abstract

“…However, the non-enzymatic way of utilizing radicals in vacuoles, which includes low-molecular species like flavonoids and antioxidants, seems to be the most important. Vacuoles contain approximately 2 mM of ascorbate (Zechmann, 2018) and approximately 100 µM of glutathione, (Pradedova et al, 2018).…”

Section: /20mentioning

confidence: 99%

Peer Review #2 of "Subcellular compartmentalization of the plant antioxidant system: an integrated overview (v0.1)"

2020

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Antioxidant system (AOS) maintains the optimal concentration of reactive oxygen species (ROS) in a cell and protects it against oxidative stress. In plants, the AOS consists of 7 main classes of antioxidant enzymes, low-molecular antioxidants (e.g., ascorbate, glutathione, and their oxidized forms) and thioredoxin/glutaredoxin systems which can serve as reducing agents for antioxidant enzymes. The number of genes encoding AOS enzymes varies between classes, and same class enzymes encoded by different gene copies may have different subcellular localizations, functional loads and modes of evolution. These facts further reinforce the complex nature of AOS regulation and functioning. Further studies can describe new trends in the behavior and functioning of systems components, and provide new fundamental knowledge about systems regulation. The system reveals to have a lot of interactions and interplay pathways between its components at the subcellular level (antioxidants, enzymes, ROS level, and hormonal and transcriptional regulation). These facts should be taken into account in further studies during the AOS modeling by describing the main pathways of generating and utilizing ROS, as well as the associated signaling processes and regulation of the system on cellular and organelle levels, which is a complicated and ambitious task. Another objective for studying the phenomenon of the AOS is related to the influence of cell dynamics and circadian rhythms on it. Therefore the AOS requires an integrated and multi-level approach to study. We focused this review on the existing scientific background and experimental data used for the systems biology research of the plant AOS.

show abstract

A novel fluorescent probe for detection of Glutathione dynamics during ROS-induced redox imbalance

Niu

Yin

et al. 2020

Analytica Chimica Acta

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Glutathione in Intact Vacuoles: Comparison of Glutathione Pools in Isolated Vacuoles, Plastids, and Mitochondria from Roots of Red Beet

Cited by 6 publications

References 24 publications

Subcellular compartmentalization of the plant antioxidant system: an integrated overview

Subcellular compartmentalization of the plant antioxidant system: an integrated overview

Peer Review #2 of "Subcellular compartmentalization of the plant antioxidant system: an integrated overview (v0.1)"

A novel fluorescent probe for detection of Glutathione dynamics during ROS-induced redox imbalance

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