Glutathione is necessary to ensure benefits of calorie restriction during ageing in Saccharomyces cerevisiae

Mannarino, Sérgio C.; Amorim, Maria Amélia; Pereira, Marcos D.; Moradas‐Ferreira, Pedro; Panek, Anita D.; Costa, Vítor Santos; Eleuthério, Elis C. A.

doi:10.1016/j.mad.2008.09.001

Cited by 32 publications

(22 citation statements)

References 48 publications

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“…In this study, through disruption of genes encoding enzyme involved in off-flavor compounds' production, the amounts of all three primary off-flavor compounds (acetaldehyde, diacetyl and pentanedione) in beer brewed with TYRL21 were reduced to a relatively lower level than that from the beer brewed with Y31, which would improve the taste of beer. Another significant aspect, beer flavor stability, which affects beer quality, is related to beer freshness (Back et al 2002) and anti-staling abilities (Mannarino et al 2008). During beer storage, oxidation causes the aging of beer, which goes on with a production of aldehyde compounds that is detrimental to beer flavor.…”

Section: Discussionmentioning

confidence: 99%

Integrated expression of the α-amylase, dextranase and glutathione gene in an industrial brewer’s yeast strain

Wang

et al. 2011

World J Microbiol Biotechnol

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Genetic engineering is widely used to meliorate biological characteristics of industrial brewing yeast. But how to solve multiple problems at one time has become the bottle neck in the genetic modifications of industrial yeast strains. In a newly constructed strain TYRL21, dextranase gene was expressed in addition of α-amylase to make up α-amylase's shortcoming which can only hydrolyze α-1,4-glycosidic bond. Meanwhile, 18s rDNA repeated sequence was used as the homologous sequence for an effective and stable expression of LSD1 gene. As a result, TYRL21 consumed about twice much starch than the host strain. Moreover TYRL21 speeded up the fermentation which achieved the maximum cell number only within 3 days during EBC tube fermentation. Besides, flavor evaluation comparing TYRL21 and wild type brewing strain Y31 also confirmed TYRL21's better performances regarding its better saccharides utilization (83% less in residual saccharides), less off-flavor compounds (57% less in diacetyl, 39% less in acetaldehyde, 67% less in pentanedione), and improved stability index (increased by 49%) which correlated with sensory evaluation of final beer product.

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Section: Discussionmentioning

confidence: 99%

Integrated expression of the α-amylase, dextranase and glutathione gene in an industrial brewer’s yeast strain

Wang

et al. 2011

World J Microbiol Biotechnol

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“…Seu metabolismo é semelhante ao de eucariotos superiores, com mecanismos próprios de ativação metabólica e de detoxificação. Apesar das diferenças de complexidade entre leveduras e humanos, o estudo do envelhecimento em leveduras tem mostrado informações importantes em vias que modulam o processo do envelhecimento em mamíferos (Mannarino et al, 2008).…”

Section: Introductionunclassified

“…Em adição, o seu pequeno tempo de geração quando comparado com as células animais e o seu genoma completamente mapeado. Por estas razões, a levedura Saccharomyces cerevisiae caracteriza-se como um dos melhores modelos de sistema eucariótico unicelular para estudos de estresse oxidativo (Mannarino et al, 2008).…”

Section: Introductionunclassified

POTENCIAL ANTIOXIDANTE DA FICOCIANINA NA LEVEDURA Saccharomyces cerevisiae MUTANTES AO GENE Sir

Balen¹,

Silveira²,

Benetti³

et al. 2015

Anais Do XX Congresso Brasileiro De Engenharia Química

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RESUMO -Compostos antioxidantes apresentam capacidades funcionais que podem proporcionar o prolongamento da vida pela atenuação do estresse oxidativo. Em vista disto, objetivou-se verificar a ação protetora da ficocianina na viabilidade celular da Saccharomyces cerevisiae mutantes ao gene sir. Utilizou-se cepas de leveduras Saccharomyces cerevisiae controle (BY4741) e deletadas aos genes sir1, sir2, sir3 e sir4. As células foram crescidas em meio YPD 2 %. As cepas foram submetidas a 24 h de envelhecimento e coletadas para as análises de sobrevivência celular. O percentual de sobrevivência foi aumentado frente ao tratamento com ficocianina nas cepas deletadas ao gene sir1, sir2 e sir4 e na cepa controle. A deleção do gene sir3, foi a que apresentou menor influência no percentual de sobrevivência frente ao tratamento com ficocianina. A cepa deletada ao gene sir2 foi a que melhor reconheceu o tratamento com ficocianina. O tratamento com ficocianina mostrou benefício na viabilidade celular. INTRODUÇÃORecentes evidências sugerem que o uso de substâncias naturais como a de diferentes compostos fenólicos, apresentam capacidades funcionais que podem proporcionar o aumento da vida pela atenuação do estresse oxidativo, inibição da incidência de patologias relativas a idade, aumento da imunidade e diminuição de processos inflamatórios. Dentre estas substâncias, o resveratrol tem sido investigado em diferentes modelos experimentais com estes objetivos. Os estudos de outras moléculas potenciais tornam-se, por estes motivos de grande importância para a potencialização destas pesquisas. Um antioxidante em potencial deve eliminar radicais livres especificamente, quelar metais; interagir com outros antioxidantes, ter um efeito positivo na expressão gênica, ser completamente absorvido, ter uma concentração relevante em tecidos e biofluidos e agir em ambos ambientes (Silva, 2007).Os antioxidantes são substâncias utilizadas na preservação dos alimentos, pois retardam a sua deterioração, rancidez e descoloração que são decorrentes do processo de oxidação. O interesse pelos antioxidantes naturais teve início nos anos 80 (Soares,

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“…The biological importance of GSH is dependent upon the redox-active free sulfhydryl moiety of its cysteine residue [3]. However, sudden exposure of eukaryotic cells to xenobiotics as herbicides may cause a redox imbalance of the cell, causing oxidative stress.…”

Section: Introductionmentioning

confidence: 99%

“…Optical density in the presence or absence of IPU, DIU and ATZ in Saccharomyces cerevisiae UE-ME3 . The results represent the mean of five independent experiments ± SEM.…”

mentioning

confidence: 99%

Effects of atrazine, isoproturon and diuron on glutathione metabolism of Saccharomyces cerevisiae

Alves-Pereira¹,

Nunes²,

Candeias³

et al. 2015

Green Processing and Synthesis

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Triazines and phenylureas, mainly used in agricultural applications for the selective control of germinating grasses and broad-leaved weeds, are often found in contaminated groundwater, surface water and the effluents of wastewater treatment plants. The toxicity of these herbicides in eukaryotic cells is poorly understood. Saccharomyces cerevisiae is a promising unicellular organism for the toxicological evaluation of xenobiotics because its metabolism is similar to that of higher-level organisms. Consequently, the aim of this study was to compare the effects of three herbicides on yeast-cell growth and the glutathione cycle. Saccharomyces cerevisiae grown in the presence of 5 or 50 μM atrazine, diuron or isoproturon were compared with control cells grown in a rich medium. The results show that the glutathione-dependent buffer capacity decreased significantly in S. cerevisiae grown in the presence of both levels of any of the three herbicides, except in cells exposed to 50 μM isoproturon. In addition, chlorine herbicides inhibited cell growth, probably due to a decrease in antioxidant power and glutathione reductase activity. Isoproturon at 50 μM induced yeast-cell growth, increasing the buffer capacity mediated by glutathione as well as glutathione reductase and glutathione peroxidase activities of UE-ME3 strain. This strain may be useful in studies of isoproturon degradation.

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Glutathione is necessary to ensure benefits of calorie restriction during ageing in Saccharomyces cerevisiae

Cited by 32 publications

References 48 publications

Integrated expression of the α-amylase, dextranase and glutathione gene in an industrial brewer’s yeast strain

Integrated expression of the α-amylase, dextranase and glutathione gene in an industrial brewer’s yeast strain

POTENCIAL ANTIOXIDANTE DA FICOCIANINA NA LEVEDURA Saccharomyces cerevisiae MUTANTES AO GENE Sir

Effects of atrazine, isoproturon and diuron on glutathione metabolism of Saccharomyces cerevisiae

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