Glutathione-Linked Thiol Peroxidase Activity of Human Serum Albumin: A Possible Antioxidant Role of Serum Albumin in Blood Plasma

Cha, Mee‐Kyung; Kim, Il-Han

doi:10.1006/bbrc.1996.0793

Cited by 118 publications

(41 citation statements)

References 2 publications

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“…Albumin is considered to be a very stable parameter, and for this reason, reduced levels of this protein are thought to be related with increased mortality risk (60,61). Moreover, a direct protective effect of this molecule has been suggested; current evidence that serum albumin may represent the major circulating antioxidant known to be exposed to continuous oxidative stress (62)(63)(64). The levels found for pregnancy are in accordance with values reported in the literature (65,66), since, especially in the third trimester of gestation in parallel with increased lipids retention, lower levels of this protein are usually observed.…”

Section: Serum Albumin Levels and Considered Subgroupsmentioning

confidence: 99%

Trace Element Status (Se, Cu, Zn) in Healthy Portuguese Subjects of Lisbon Population: A Reference Study

Lopes

Santos²,

Vicente

et al. 2004

BTER

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Serum levels of selenium, copper, and zinc were systematically determined in healthy subjects of the Lisbon population. The sample consisted of 183 blood donors of both genders who were divided into two age groups: 20-44 and 45-70 yr of age; relationships with gender, age, the lipid profile, and tobacco consumption were investigated. In the female group, the intake of oral contraceptives and pregnancy were considered for the youngest women, and hormonal replacement therapy (HRT) was taken into account for the oldest ones. Serum concentrations of these elements were in the same range as those found for populations of other European countries. Differences between genders were observed for the three elements studied, with serum selenium and zinc concentrations higher in men and copper levels higher in women. Age-dependent differences were found for selenium: The oldest subjects (regardless gender) presented the highest concentrations of * Author to whom all correspondence and reprint requests should be addressed.selenium in serum as further demonstrated by the positive correlation with age. In both pregnant and contraceptive-using women, copper was greatly increased, confirming the influence of estrogen status and/or oral contraceptive intake on increased serum copper levels. However, in postmenopausal women, HRT did not significantly affect serum copper levels. Selenium, copper, and zinc status were not different between normolipidemic and hyperlipidemic subjects for the same gender and age range, but selenium levels tended to increase with hyperlipidemia when considering the whole group of subjects. With respect to the lifestyle, higher serum zinc levels were found in tobacco-consuming men. Albumin serum levels were similar for all considered subgroups, except for the pregnant women, for whom a decrease in this parameter was observed. The present study allowed one to obtain reference values for this healthy group of population, which will serve for a comparative study with groups having pathological conditions, such as cardiovascular disease.

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Section: Serum Albumin Levels and Considered Subgroupsmentioning

confidence: 99%

Trace Element Status (Se, Cu, Zn) in Healthy Portuguese Subjects of Lisbon Population: A Reference Study

Lopes

Santos²,

Vicente

et al. 2004

BTER

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“…In addition, a glutathione-linked thiol peroxidase activity (26) and a carbon radical scavenging activity (27) have been described for albumin as well as its capability to scavenge peroxyl radicals and to decrease lipoxygenase activity (25). As reported recently, the antioxidant properties of serum albumin are impaired by free radicals (28).…”

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confidence: 94%

Antioxidant Status and Nitric Oxide in the Malnutrition Syndrome Kwashiorkor

et al. 2001

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“…Human serum albumin (HSA) is a single nonglycosylated polypeptide containing 35 cysteine residues all involved in the formation of stabilising disulphide bonds except 34 cysteine. In plasma, this free thiol group is quantitatively the most important scavenger of oxidants (4)(5)(6), and is thus an important antioxidant within the body (7).…”

Section: Introductionmentioning

confidence: 99%

Oxidative modification of albumin in the parenchymal lung tissue of current smokers with chronic obstructive pulmonary disease

et al. 2010

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Background: There is accumulating evidence that oxidative stress plays an important role in the pathophysiology of chronic obstructive pulmonary disease (COPD). One current hypothesis is that the increased oxidant burden in these patients is not adequately counterbalanced by the lung antioxidant systems. Objective: To determine the levels of oxidised human serum albumin (HSA) in COPD lung explants and the effect of oxidation on HSA degradation using an ex vivo lung explant model. Methods: Parenchymal lung tissue was obtained from 38 patients (15F/23M) undergoing lung resection and stratified by smoking history and disease using the GOLD guidelines and the lower limit of normal for FEV 1 /FVC ratio. Lung tissue was homogenised and analysed by ELISA for total levels of HSA and carbonylated HSA. To determine oxidised HSA degradation lung tissue explants were incubated with either 200 μg/ml HSA or oxidised HSA and supernatants collected at 1, 2, 4, 6, and 24 h and analysed for HSA using ELISA and immunoblot. Results: When stratified by disease, lung tissue from GOLD II (median = 38.2 μg/ml) and GOLD I (median = 48.4 μg/ml) patients had lower levels of HSA compared to patients with normal lung function (median = 71.9 μg/ml, P < 0.05). In addition the number of carbonyl residues, which is a measure of oxidation was elevated in GOLD I and II tissue compared to individuals with normal lung function (P < 0.05). When analysing smoking status current smokers had lower levels of HSA (median = 43.3 μg/ml, P < 0.05) compared to ex smokers (median = 71.9 μg/ml) and non-smokers (median = 71.2 μg/ml) and significantly greater number of carbonyl residues per HSA molecule (P < 0.05). When incubated with either HSA or oxidised HSA lung tissue explants rapidly degraded the oxidised HSA but not unmodified HSA (P < 0.05). Conclusion: We report on a reliable methodology for measuring levels of oxidised HSA in human lung tissue and cell culture supernatant. We propose that differences in the levels of oxidised HSA within lung tissue from COPD patients and current smokers provides further evidence for an oxidant/antioxidant imbalance and has important biological implications for the disease.

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Glutathione-Linked Thiol Peroxidase Activity of Human Serum Albumin: A Possible Antioxidant Role of Serum Albumin in Blood Plasma

Cited by 118 publications

References 2 publications

Trace Element Status (Se, Cu, Zn) in Healthy Portuguese Subjects of Lisbon Population: A Reference Study

Trace Element Status (Se, Cu, Zn) in Healthy Portuguese Subjects of Lisbon Population: A Reference Study

Antioxidant Status and Nitric Oxide in the Malnutrition Syndrome Kwashiorkor

Oxidative modification of albumin in the parenchymal lung tissue of current smokers with chronic obstructive pulmonary disease

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