Glutathione peroxidase-1 and klotho gene expression in acute renal failure rats and its association with creatinine and urea levels

Abstract

The main objectives of this work was to study the effect of glycerol induced acute renal injury on Glutathione peroxidase (GPx-1) and klotho gene expression which used as early indicator for acute renal failure. Twelve male albino rats divided into two groups were included in this study. Blood samples were taken for assessment of creatinine and blood urea nitrogen. Kidney tissue specimens were processed for mRNA gene expression study of Glutathione peroxidase (GPx-1) and klotho genes by real time polymerase chain reaction (RT-PCR). The creatinine and blood urea nitrogen significantly increased (4.97± 0.75 and 242.03 ± 41.93) compared with control group (0.67 ± 0 .06 and 24.43 ± 2.18). Moreover, the renal Glutathione peroxidase-1 and klotho gene expression was found to be decreased in the glycerol treated group (affected group), to 0.899 and 0.91fold. Also a correlation between gene expression of Glutathione peroxidase-1and klotho gene with the level of creatinine and blood urea nitrogen was detected.