Glutathione Peroxidase Blockage Inhibits Prostaglandin Biosynthesis in Rat Platelets and Aorta

Doni, Maria Gabriella; Piva, Elisa

doi:10.1159/000214754

Cited by 4 publications

(1 citation statement)

References 4 publications

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“…Moreover, SOD expression has been observed to be increased by TNF-α in a mechanism involving NF-κB activation [ 38 ]. Although we cannot rule out the possibility of partial inhibition of GPX by 3-AT [ 39 ], most of the studies using 3-AT report a specific inhibition of catalase [ 40 , 41 ]. Additionally, the expression levels of PRDX1 , PRDX3 and PRDX5 , which are enzymes that are also involved in H 2 O 2 clearance, were significantly reduced after treatment with 3-AT, which could contribute to increase H 2 O 2 levels.…”

Section: Discussionmentioning

confidence: 99%

Impact of 3-Amino-1,2,4-Triazole (3-AT)-Derived Increase in Hydrogen Peroxide Levels on Inflammation and Metabolism in Human Differentiated Adipocytes

et al. 2016

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Obesity is characterized by an excessive accumulation of fat in adipose tissue, which is associated with oxidative stress and chronic inflammation. Excessive H2O2 levels are degraded by catalase (CAT), the activity of which is decreased in obesity. We investigated the effects of inhibition of catalase activity on metabolism and inflammation by incubating human differentiated adipocytes with 10 mM 3-amino-1,2,4-triazole (3-AT) for 24 h. As expected, the treatment decreased CAT activity and increased intracellular H2O2 levels significantly. Glutathione peroxidase (GPX) activity was also reduced, and the gene expression levels of the antioxidant enzymes GPX4 and peroxiredoxins (1, 3 and 5) were inhibited. Interestingly, this occurred along with lower mRNA levels of the transcription factors nuclear factor (erythroid 2-like 2) and forkhead box O, which are involved in redox homeostasis. However, superoxide dismutase activity and expression were increased. Moreover, 3-AT led to nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) activation and increased tumor necrosis alpha and interleukin 6 protein and gene expression levels, while lowering peroxisome proliferator-activated receptor gamma (PPARγ) mRNA and protein levels. These alterations were accompanied by an altered glucose and lipid metabolism. Indeed, adipocytes treated with 3-AT showed reduced basal glucose uptake, reduced glucose transporter type 4 gene and protein expression, reduced lipolysis, reduced AMP-activated protein kinase activation and reduced gene expression of lipases. Our results indicate that increased H2O2 levels caused by 3-AT treatment impair the antioxidant defense system, lower PPARγ expression and initiate inflammation, thus affecting glucose and lipid metabolism in human differentiated adipocytes.

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Section: Discussionmentioning

confidence: 99%

Impact of 3-Amino-1,2,4-Triazole (3-AT)-Derived Increase in Hydrogen Peroxide Levels on Inflammation and Metabolism in Human Differentiated Adipocytes

et al. 2016

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Kidney glomerular explants in serum-free media: Demonstration of intracellular antioxidant enzymes and active oxygen metabolites

Steinert

Anderson

Oberley

et al. 1986

In Vitro Cell Dev Biol

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Guinea pig glomeruli were grown for 22 d in a serum-free medium composed of Waymouth's MB 752/l supplemented with sodium pyruvate, nonessential amino acids, and antibiotics (the basic medium). Intracellular cellular activity of the antioxidant enzymes superoxide dismutase (SOD; both copper-zinc [Cu,Zn] and manganese [Mn] forms) and catalase, and intracellular active oxygen metabolites (hydrogen peroxide [H2O2] and superoxide [O2-.]) were measured with time in culture. The results were compared to results obtained from glomeruli grown in different serum-free media, including the basic medium plus fibronectin (FN), the basic medium plus transferrin and FN, and a complex medium containing insulin, transferrin, selenium (Se), triiodothyronine, and FN (complete medium). In general, although the intracellular activity of antioxidant enzymes and active oxygen metabolites varied over time in culture in all media, there were only a few statistically significant differences among different media. Both CuZn SOD and Mn SOD activity were demonstrated in isolated glomeruli. The CuZn SOD activity per DNA ratio decreased slightly with time in culture in all media tested except the complete medium, in which CuZn SOD activity per DNA ratio remained more constant. The Mn SOD activity per DNA ratio did not vary significantly over time in culture. Catalaselike activity was very low in isolated glomeruli and declined sharply with time in culture in all media except the complete medium. Both H2O2 and O2-. were detected intracellularly in glomerular culture. Our results indicate that intracellular antioxidant enzymes and active oxygen metabolites in glomeruli vary with time in culture and, in some instances, with culture conditions.

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Activity of placental glutathione peroxidase and superoxidedismutase in cows with and without retained fetal membranes

Kankofer¹,

M²,

Fidecki³

et al. 1996

Placenta

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Glutathione Peroxidase Blockage Inhibits Prostaglandin Biosynthesis in Rat Platelets and Aorta

Cited by 4 publications

References 4 publications

Impact of 3-Amino-1,2,4-Triazole (3-AT)-Derived Increase in Hydrogen Peroxide Levels on Inflammation and Metabolism in Human Differentiated Adipocytes

Impact of 3-Amino-1,2,4-Triazole (3-AT)-Derived Increase in Hydrogen Peroxide Levels on Inflammation and Metabolism in Human Differentiated Adipocytes

Kidney glomerular explants in serum-free media: Demonstration of intracellular antioxidant enzymes and active oxygen metabolites

Activity of placental glutathione peroxidase and superoxidedismutase in cows with and without retained fetal membranes

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