Activity of placental glutathione peroxidase and superoxidedismutase in cows with and without retained fetal membranes

Kankofer, Marta; M, Podolak; Fidecki, M.; Gondek, T.M.

doi:10.1016/s0143-4004(96)80076-2

Cited by 18 publications

(10 citation statements)

References 29 publications

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“…In cows affected with RFM (groups B, D, F) the concentrations were significantly lower (p < 0.05) than in cows without RFM (groups A, C, E). Our earlier observations showed that GSH‐Px activities were significantly lower in not‐retained placenta compared to retained placenta collected shortly after parturition (Kankofer et al. 1996).…”

Section: Discussionmentioning

confidence: 92%

Non-enzymatic Antioxidative Defence Mechanisms Against Reactive Oxygen Species in Bovine-retained and not-retained Placenta: Vitamin C and Glutathione

Kankofer¹

2001

Reprod Domest Anim

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Vitamin C and glutathione (GSH) are water-soluble antioxidants which take part in defence mechanisms against reactive oxygen species (ROS). They may also be involved in processes of releasing/retaining bovine fetal membranes. Hence vitamin C, reduced (GSH) and oxidised (GSSG) glutathione levels were determined in retained and not-retained bovine fetal membranes in order to describe the non-enzymatic antioxidative status. Placental samples were collected immediately after spontaneous delivery or during caesarean section before term and at term, and 6 groups were formed as follows: (A) pre-term caesarean section without retained placenta; (B) pre-term caesarean section with retained placenta; (C) term caesarean section without retained placenta; (D) term caesarean section with retained placenta; (E) spontaneous delivery without retained placenta and (F) spontaneous delivery with retained placenta. Homogenates of maternal and fetal placental tissues were prepared, and vitamin C, GSH and GSSG were measured spectrophotometrically. Vitamin C levels were significantly higher in the maternal part than in the fetal part of the placenta in all groups examined. In retained placenta cases the levels were significantly lower than in control cows, except in pre-term groups. GSH concentrations were significantly higher in placentas without retention than with retention. GSSG levels showed the opposite relationship and were significantly higher in samples with retention of fetal membranes than in controls. Further experiments on antioxidative as well as oxidative status in bovine placenta are necessary.

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Section: Discussionmentioning

confidence: 92%

Non-enzymatic Antioxidative Defence Mechanisms Against Reactive Oxygen Species in Bovine-retained and not-retained Placenta: Vitamin C and Glutathione

Kankofer¹

2001

Reprod Domest Anim

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“…While total GSH‐Px values were higher in tissues of retained placenta than in released as well as higher in foetal as in maternal part in both groups (Kankofer et al. ).…”

Section: Discussionmentioning

confidence: 95%

The Presence of SOD 1 and GSH‐Px in Bovine Retained and Properly Released Foetal Membranes

Kankofer

Wawrzykowski

Hoedemaker

2013

Reprod Domestic Animals

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The maintenance of antioxidative/oxidative balance is crucial for cellular and extracellular environment. That is why antioxidative enzymes express their activity in different isoforms in different cell compartments and extracellular space. The aim of study was to verify the results of previous experiment on activities of antioxidative enzymes by the determination of their enzymatic proteins in bovine placental tissues by Western blotting technique. Moreover, the presence of particular isoenzymes was detected and differentiated. Homogenates of maternal and foetal part of both properly released and retained bovine placenta were subjected to PAGE electrophoresis in non-reducing and reducing conditions and Western blotting with appropriate antibodies against superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px). Electrophoresis allowed for the detection of protein bands of molecular weight related to CuZn-SOD as well as cGSH-Px isoenzymes. The reaction with appropriate antibodies confirmed this. Densitometric analysis, although semi-quantitative, allowed for the observation of trends in differences in antioxidative enzyme proteins, which may partly confirm previously described results in cases of retained and released placenta. Local antioxidative enzymatic mechanisms in bovine placental tissues are represented by CuZn-SOD and cGSH-Px, which show the changes in their expression during improper placental release.

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“…Furthermore, the activity of anti-oxidative enzymes in placental tissues is altered in cases of retained foetal membranes (Kankofer et al, 1996), which may con®rm the imbalance of the oxidative/antioxidative status.…”

Section: Discussionmentioning

confidence: 99%

Protein Peroxidation Processes in Bovine Retained and Not‐retained Placenta

Kankofer

2001

Journal of Veterinary Medicine Series A

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Proteins as well as lipids and other biologically active substances may undergo peroxidative damage caused by reactive oxygen species (ROS). The aim of this study was the determination of this damage in cases of retained (RFM) and not-retained bovine foetal membranes. The levels of thiol groups were measured spectrophotometrically. Tryptophan, formylokinurenine and bityrosine bridges were determined using spectrofluorimetric methods. All parameters were determined in placental maternal and foetal homogenates collected from cows divided into six groups as follows: A, pre-term caesarian section without RFM; B, pre-term caesarian section with RFM; C, term caesarian section without RFM; D, term caesarian section with RFM; E, spontaneous delivery without RFM; F, spontaneous delivery with RFM. All parameters except tryptophan showed statistically significant differences between the maternal and foetal parts of the placenta. The concentrations of the thiol groups were lower in RFM than in not-RFM cases, whereas formylokinurenine and bityrosine concentrations showed the opposite relationship. Tryptophan levels were lower in cases of RFM rather than not-RFM samples but statistically significant differences occurred in groups C and D and the foetal part of groups E and F. Further experiments are necessary to determine the oxidative status of other biologically active substances in cases of retained bovine placenta.

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Activity of placental glutathione peroxidase and superoxidedismutase in cows with and without retained fetal membranes

Cited by 18 publications

References 29 publications

Non-enzymatic Antioxidative Defence Mechanisms Against Reactive Oxygen Species in Bovine-retained and not-retained Placenta: Vitamin C and Glutathione

Non-enzymatic Antioxidative Defence Mechanisms Against Reactive Oxygen Species in Bovine-retained and not-retained Placenta: Vitamin C and Glutathione

The Presence of SOD 1 and GSH‐Px in Bovine Retained and Properly Released Foetal Membranes

Protein Peroxidation Processes in Bovine Retained and Not‐retained Placenta

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