Glutathione peroxidase, glutathione reductase, Cu–Zn superoxide dismutase activities, glutathione, nitric oxide, and malondialdehyde concentrations in serum of patients with chronic lymphocytic leukemia

Bakan, Nuri; Taysı, Seyithan; Yılmaz, Ömer; Bakan, Ebubekîr; Kuşkay, Sevinç; Uzun, Naim; Gündoğdu, Mehmet

doi:10.1016/j.cccn.2003.08.013

Cited by 78 publications

(49 citation statements)

References 42 publications

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“…1). Previous studies measuring GR activity and GSH level in serum indicated a decrease in both parameters in chronic lymphocytic leukemia patients [14], and significant fluctuation of GR and GSH in head and neck cancer patients during anticancer therapy [15]. However, according to the best knowledge of the authors, evaluation of both antioxidants were not performed in serum of patients simultaneously with various stages of CHD.…”

Section: Resultsmentioning

confidence: 90%

Glutathione level and glutathione reductase activity in serum of coronary heart disease patients

Zuzak¹,

Horecka²,

Kiełczykowska³

et al. 2017

J Pre Clin Clin Res.

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level and glutathione reductase activity in serum of coronary heart disease patients. J Pre-Clin Clin Res. 2017; 11(2): 103-105. doi: 10.26444/jpccr/81277 Abstract Introduction. Oxidative stress induced by the generation of excess reactive oxygen species is one of the cause of atherosclerosis finally leading to coronary heart disease (CHD). Glutathione reductase (GR), a flavoprotein antioxidant enzyme, regenerates glutathione (GSH) from its oxidized form, which is essential for scavenging of hydrogen peroxide by glutathione peroxidase. The aim of this study was to analyze the activity of GR and GSH level in serum of patients with various stages of CHD. Materials and method. Sixty consecutive patients with diagnosis of stable angina (SA, n=20), unstable angina (UA, n=20) and non-fatal myocardial infarction (MI, n=20) together with 20 healthy control subjects were enrolled. Venous blood samples were collected just before the coronary catheterization procedure (in study patients). GR activities and GSH concentration was determined using commercially available colorimetric kits. Results. The highest GR activity and GSH serum level was noticed in UA patients. However, statistical evaluation showed that only glutathione reductase activity was significantly higher in serum obtained from myocardial infarction patients, compared to stable angina pectoris patients (19.52 ± 13.88 vs 11.63 ± 5.45 nmol/min/ml for MI and SA, respectively; p<0.048). There were no significant differences in GSH between the CHD patients and controls. Conclusion. The elevated activity of glutathione reductase in serum of patients with unstable angina pectoris and myocardial infarction suggests the role of antioxidant system acute coronary syndromes.

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Section: Resultsmentioning

confidence: 90%

Glutathione level and glutathione reductase activity in serum of coronary heart disease patients

Zuzak¹,

Horecka²,

Kiełczykowska³

et al. 2017

J Pre Clin Clin Res.

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“…Endogenous NO is of a dual role in specialized tissues and cells; it is an essential physiological signalling molecule not only mediating various cell functions but also inducing cytotoxic and mutagenic effects when present in excess. NO reacts rapidly with superoxide anion to form peroxynitrite, which may be cytotoxic by itself or easily decompose to the highly reactive and toxic hydroxyl radical and nitrogen dioxide [12] .…”

Section: Introductionmentioning

confidence: 99%

Protective Effects of Increasing Vitamin E and A Doses on Cisplatin-Induced Oxidative Damage to Kidney Tissue in Rats

Dillioğlugil¹,

Kır²,

Gülkaç

et al. 2005

Urol Int

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Objective: Cisplatin (DDP, cis-diamminedichloroplatinium II) is one of the most potent chemotherapeutic antitumor drugs, but is able to generate reactive oxygen species (ROS) and it also inhibits the activity of antioxidant enzymes in renal tissue. In the present study, we investigated the preventive effect of 100, 200 and 400 mg/kg b.w. doses of vitamin E (VE), and 25, 50, and 100 mg/kg b.w. doses of vitamin A (VA) combination on malondialdehyde (MDA), nitric oxide (NO), and glutathione (GSH) levels and superoxide dismutase (SOD) activity in cisplatin-induced toxicity in rat kidneys. Our literature survey indicated a lack of any experimental study showing the beneficial effect of VA on cisplatin-induced MDA, NO, GSH and SOD changes. For this reason, we hoped that this study would provide a unique contribution in that respect. Materials and Methods: 59 Wistar rats (11 to replace prematurely lost animals) were used. 48 evaluable rats were divided into 8 groups (n = 6 in each group): control group, DDP alone (5 mg/kg b.w.) group, 3 VE combination treatment groups of VE100+DDP, VE200+DDP, and VE400+DDP, and 3 VA combination treatment groups of VA25+DDP, VA50+DDP, and VA100+DDP. Kidney MDA, GSH, NO levels and SOD activities were determined for the assessment of oxidant-antioxidant balance. Results: While in the DDP group the tissue levels of MDA and NO were found to be significantly higher than in the control group, GSH levels and SOD activities were significantly lower. MDA and NO levels were found to be significantly lower and GSH levels and SOD activities significantly higher in the VE200+DDP and VE400+ DDP groups when compared with the DDP alone group. MDA and NO levels were found to be significantly lower in the VA50+DDP and VA100+DDP groups when compared with the DDP alone group. However, identical comparisons with the DDP alone group showed significantly higher GSH levels and SOD activities in the VA25+DDP, VA50+DDP, and VA100+DDP groups. Among the VE100+ DDP, VE200+DDP, and VE400+DDP groups, and VA25+ DDP, VA50+DDP, and VA100+DDP groups, MDA and NO levels decreased and GSH levels and SOD activities increased steadily and significantly as the doses of VE and VA increased. Conclusion: These vitamins would be effective in protecting against cisplatin-induced tissue damage in rat kidneys. It is possible that the toxic effect of cisplatin is somehow minimized by a compensatory mechanism involving VE and VA via induction of antioxidant enzyme activities following intraperitoneal injection of DDP.

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“…Praćenjem aktivnosti superoksid dizmutaze u plazmi obolelih od Cll i poređenjem sa vrednostima dobijenim za isti parametar u kontrolnoj grupi, u našem istraživanju pokazano je da ne postoje statistički značajne razlike između tih dveju grupa ispitanika. Za razliku od nas, grupa turskih istraživača (Bakan i saradnici, 2003) pokazala je da u serumu obolelih od Cll postoji statistički značajan pad aktivnosti ne samo superoksid dizmutaze nego i glutation peroksidaze (20). u zavisnosti od uslova, glavni regulator koncentracije vodonik-peroksida je enzim katalaza, dok glutation-peroksidaza i hemoglobin imaju ograničenu ulogu.…”

Section: Slika 4 Aktivnosti Superoksid Dizmutaze U Plazmiunclassified

“…dobijeni rezultati su u saglasnosti sa literaturnim podacima. Bakan i saradnici (2003) su takođe pokazali pad serumske aktivnosti glutation peroksidaze kod obolelih od hronične limfocitne leukemije (20). i kod drugih vrsta tumora dobijeni su slični rezultati, arivazagan i saradnici (1997) su pokazali pad aktivnosti glutation peroksidaze kod pacijenata sa kancerom želuca (30).…”

Section: Slika 4 Aktivnosti Superoksid Dizmutaze U Plazmiunclassified

Activity of superoxide dismutase, catalase and glutathione peroxidase in plasma and lysates of chronic lymphocytic leukemia patients

Zarić¹,

Mitrović²,

Nikolić³

et al. 2011

Medicinski casopis

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UvodHronična limfocitna leukemija (chronic lymphocytic leukemia -Cll) je maligna bolest hematopoeznog tkiva koja nastaje proliferacijom i akumulacijom malih, naizgled zrelih, imunološki nekompetentnih, dugoživećih limfocita u kostnoj srži, limfnim nodusima, slezini i drugim organima (1).Slobodni radikali kiseonika su intermedijerni proizvodi nepotpune redukcije kiseonika u ćeliji. njihova produkcija je u fiziološkim uslovima kontrolisana, a sama ćelija poseduje enzimske i neenzimske sisteme odbrane od slobodnih radikala. glavni antioksidativni enzimi kojima ćelija neutrališe slobodne radikale kiseonika jesu superoksid dizmutaza (SOd), glutation peroksidaza (gPx) i katalaza (CaT). u uslovima nekontrolisanog stvaranja slobodnih radikala njihova količina može da prevaziđe antioksidantni kapacitet ćelije i tada nastaje oksidativni stres. danas se smatra da oksidativni stres ima značajnu ulogu u patogenezi velikog broja oboljenja, kao i u samoj kancerogenezi (2).Stoga je cilj našeg istraživanja bio da se odrede aktivnosti glavnih antioksidativnih enzima u plazmi i

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Glutathione peroxidase, glutathione reductase, Cu–Zn superoxide dismutase activities, glutathione, nitric oxide, and malondialdehyde concentrations in serum of patients with chronic lymphocytic leukemia

Cited by 78 publications

References 42 publications

Glutathione level and glutathione reductase activity in serum of coronary heart disease patients

Glutathione level and glutathione reductase activity in serum of coronary heart disease patients

Protective Effects of Increasing Vitamin E and A Doses on Cisplatin-Induced Oxidative Damage to Kidney Tissue in Rats

Activity of superoxide dismutase, catalase and glutathione peroxidase in plasma and lysates of chronic lymphocytic leukemia patients

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