Glutathione S-transferase activity in rats exposed to methyl parathion

Abstract

Methyl parathion is an organophosphate insecticide that has been used in agriculture and the domestic sector for several years. This pesticide and others, arriving through different processes, exert significant effects on water quality with serious consequences for environmental and human health. The main objective of this study was to investigate the changes of Glutathione S-transferase enzyme activity in methyl parathion exposed rat tissues. For this purpose, wistar rats (Rattus norvegicus) were injected intraperitoneally with 7 mg kg −1 dose of methyl parathion, while corn oil was applied to control groups in the same way. The liver, kidneys, brain and small intestine were quickly removed after 0, 2, 4, 8, 16, 32, 64, and 72 hours of injection of methyl parathion and the glutathione S-transferase activity was determined in these tissues. As a result it was seen that glutathione S-transferase activity increases in all tissues in the group of male and female rats to which methyl parathion was given. The increase of glutathione S-transferase activity may be a result of methyl parathion's toxic effect because it is one of the most important enzymes of detoxification metabolism.

Effect of the sterol demethylation-inhibiting fungicide fenarimol on selected biochemical parameters in rats

“…In addition, chronic exposure of rats and mice to pesticide led to increased levels of serum ALT and AST (Gomes et al, 1999). Our present data, in agreement with those from a previous study, found that dinitro-o-cresol, dichlorvos, and methyl parathion, which are all used as pesticides, caused increases and decreases in serum enzyme activities and some biochemical parameters in female and male rats (Dere et al 2007;Ozdikicioglu et al, 2008;Ari and Dere, 2008). Our present data, in agreement with those from a previous study, found that dinitro-o-cresol, dichlorvos, and methyl parathion, which are all used as pesticides, caused increases and decreases in serum enzyme activities and some biochemical parameters in female and male rats (Dere et al 2007;Ozdikicioglu et al, 2008;Ari and Dere, 2008).…”

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Fenarimol,a 2, benzhydryl alcohol fungicide and steroid demethylation inhibitor, has been reported to affect the actions of multiple CYP450 isoforms, including key enzymes. The aim of this study was to evaluate the effects of fenarimol on a set of biochemical parameters in rats.

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Fenarimol,a 2,

benzhydryl alcohol fungicide and steroid demethylation inhibitor, has been reported to affect the actions of multiple CYP450 isoforms, including key enzymes. Serum was analysed at 0,2,4,8,16,32, 64 and 72 h following injection. Rats were intraperitoneally injected with a 200 mg kg -1 dose of fenarimol.

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“…Serum was analysed at 0,2,4,8,16,32, 64 and 72 h following injection. The aim of this study was to evaluate the effects of fenarimol on a set of biochemical parameters in rats.…”

Glutathione S-Transferase Activity in Tissues of Rats Exposed to Fenarimol

“…To understand whether pesticides affect non-target organisms, enzyme activities in damaged tissues can be examined [28][29][30][31][32]. Although there are studies on the GST activity of pesticides in the literature [33][34][35][36], there is no study on the GST activity of fenarimol in rat tissues. Therefore, it is important to know the effect of fenarimol on rats.…”