Glutathione S-transferase gene polymorphisms (GSTM1, GSTT1, GSTP1) and prostate cancer: a case-control study in Tehran, Iran

Abstract

We evaluated the relationship between polymorphisms in the glutathione S-transferases (GSTs) GSTM1, GSTT1 and GSTP1 genes and prostate cancer (PCa). PCR-restriction fragment length polymorphism assay was used to genotype the GSTM1, GSTT1, and GSTP1 polymorphisms in 168 PCa cases and 336 frequency matched controls. The GSTM1 null, and GSTT1 null genotypes were associated with an increased odds ratio (OR) for PCa (OR ¼ 3.28, 95% confidence interval (CI): 2.47-5.64; P ¼ 0.005, and OR ¼ 3.21, 95% CI: 2.52-5.64; P ¼ 0.005, respectively) (Pcorrected ¼ 0.0062). The frequency of GSTP1 Val/Val genotype was 14.3% in cases compared with 2.4% in controls, this polymorphism thus being associated with a significantly increased risk of PCa (OR ¼ 3.72, 95% CI: 1.67-5.65; P ¼ 0.002). The risk associated with the concurrent absence of both of the genes (OR ¼ 4.8, 95% CI: 2.34-6.78) was greater than the product of risk in men with either null (OR ¼ 1.52, 95% CI: 0.82-2.31) genotype combinations (P ¼ 0.001, Pcorrected ¼ 0.0045). The combination of GSTP1 Ile/Val or Val/Val polymorphism with the GSTT1 null and GSTM1 null type resulted in an OR of 6.21 (95% CI: 4.83-16.87) (P ¼ 0.0001, Pcorrected ¼ 0.0062). A higher frequency of the GSTM1 null genotype and GSTT1 null genotype was observed in patients with Gleason score 47, with an OR for GSTM1 null 4.67 (95% CI: 3.64-7.62; P ¼ 0.001) and with an OR for GSTT1 null 3.62 (95% CI: 2.31-5.74; P ¼ 0.004). The results obtained demonstrated that simultaneous presence of three potentially risk alleles (GSTM1 null, GSTT1 null and GSTP1 Val) lead to a significant OR increase for PCa.

Türk Toplumunda GSTM1, GSTP1 ve GSTT1 Gen Polimorfizmlerinin Prostat Kanseri Riski Üzerine Etkileri

“…17,18 A more recent study also demonstrated that GSTM1 null and GSTT1 null genotype was observed in a higher frequency in patients with a Gleason score >7. 19 A detailed analysis of GST gene polymorphisms and their association with clinicopathological parameters in prostatectomy specimens does not exist in our population. Therefore, whether these genes have prognostic impact on prostate cancer patients remains unknown for Turkish men.…”

Genetic Polymorphisms of Glutathione S-transferase M1 and Prostate Cancer Risk in Asians: A Meta-analysis of 18 Studies

“…Of these studies, 9 reported on Caucasians, and 9 reported on East Asians. Studies were carried out in Japan (Murata et al, 2001;Nakazato et al, 2003;Komiya et al, 2005), China (Guan et al, 2005;Lai et al, 2005;Wang et al, 2005;Yang et al, 2006;Li et al, 2008), India ( Vijayalakshmi et al, 2005;Srivastava et al, 2005;Mittal et al, 2006;Thakur et al, 2011;Kumar et al, 2011), South Korea (Kwon et al, 2011), Iran (Ashtiani et al, 2011;Safarinejad et al, 2011) and Turkey (Aktas et al, 2004;Silig et al, 2006). A list of details abstracted from the studies included in the meta-analysis is provided through Table 1.…”

Lung Adenocarcinoma and Squamous Cell Carcinoma in association with Genetic Polymorphisms of GSTs in Slovak Population

“…GSTP1 Ile105Val polymorphism modifies the kinetic properties of coded enzyme (16,17) and some other studies demonstrated higher sensibility for carcinomas in individuals bearing the variant valine allele (18).…”