Glutathione Transferase in Bacteria: Subunit Composition and Antigenic Characterization

Immunogold localization of glutathione transferase B1‐1 in Proteus mirabilis

“…Thus, our results strongly support the hypothesis of the inactivating role of this enzymatic system against several classes of antibiotics [19,23] which represent a novel aspect of the general detoxification role of GST.…”

“…Proteins were electrophoretically transferred from polyacrylamide gel onto nitrocellulose membrane (Bio-Rad Transblot System) according to the method of Towbin et al [18], with sligth modiication previously described [19].…”

Degradation of furazolidone by bacteria Acinetobacter calcoaceticus T32, Pseudomonas putida SP1 and Proteus mirabilis V7

“…GSTs are part of a superfamily of enzymes that play a key role in cellular detoxification. GSTs are widely distributed in prokaryotes and bacterial GSTs are implicated in a variety of distinct processes such as the biodegradation of xenobiotics, protection against chemical and oxidative stresses and antimicrobial drug resistance (Piccolomini et al, 1989;Vuilleumier, 1997;Allocati et al, 2009). The result obtained in this study indicated that bacterial GST activity was influenced by the presence of different FZD concentrations and thus GST might be involved in FZD degradation by bacterial strains.…”

The distribution of homologues of the Escherichia coli KefC K+-efflux system in other bacterial species

“…Other activators of the KefC system can only be synthesized through the intervention of the glutathione S-transferase (GST), for example 2,4-dinitrophenyl-S-glutathione formed from chlorodinitrobenzene (Elmore et al, 1990). While it is known that GSTs are widespread in Gram-negative bacteria there does not appear to have been a systematic evaluation of their presence in Gram-positive bacteria (Piccolomini et al, 1989). The role of GST in Gramnegative bacteria is not completely resolved since there are no mutants lacking this enzyme at the present time.…”