Glutathione-Triggered catalytic response of Copper-Iron mixed oxide Nanoparticles. Leveraging tumor microenvironment conditions for chemodynamic therapy

Bonet-Aletá, Javier; Sancho‐Albero, María; Calzada-Funes, Javier; Irusta, Silvia; Martín-Duque, Pilar; Hueso, José L.; Santamarı́a, Jesús

doi:10.1016/j.jcis.2022.03.036

Cited by 36 publications

(45 citation statements)

References 78 publications

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“…5b ). The corresponding GSH intracellular levels were quantified following a recently optimized protocol by Bonet-Aleta et al 32 (see Experimental section). Both cancer cell lines ( i.e.…”

Section: Resultsmentioning

confidence: 99%

“…CuFe 2 O 4 nanoparticles were synthesized via a templated-growth method. 32 Firstly, 250 mg of BSA were dissolved in 2.5 mL of Milli-Q water and consequently 12.5 mL of ethylene glycol were added. After that, 270.0 mg of FeCl 3 ·6H 2 O, 85.0 mg of CuCl 2 and 375.0 mg of CH 3 COONa were added into the reaction vessel.…”

Section: Methodsmentioning

confidence: 99%

“…Analysis of the intracellular GSH levels was performed as follows, based on a previously optimized protocol. 32 Briefly, cells were first trypsinized, washed twice with PBS (500 g for 5 minutes) and collected. Cells were again centrifuged at 13 300 rpm for 5 minutes.…”

Section: Methodsmentioning

confidence: 99%

“…A concentration of GSH of 5 mM was selected for the experiments in order to mimic the average intracellular GSH levels reported in the literature for cancer cells. [32][33][34][35] The presence of GSH at a concentration of 5 mM changed considerably the leaching patterns. Thus, the release of Cu was strongly enhanced, with a cumulative release of ca.…”

Section: Cation Leaching In the Presence Of Gshmentioning

confidence: 99%

“…† Samples were analyzed by UPLC-PDA equipment following the analysis conditions presented before. For the calculation of intracellular GSH, an average volume of 1.2 Â 10 À11 L, 3.3 Â 10 À12 L and 2.6 Â 10 À12 L were selected for hpMSCs, U251-MG and HeLa cells, respectively 32,93. Statistical analysisResults are expressed as mean AE standard deviation.…”

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confidence: 99%

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Unveiling the interplay between homogeneous and heterogeneous catalytic mechanisms in copper–iron nanoparticles working under chemically relevant tumour conditions

et al. 2022

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“…5b ). The corresponding GSH intracellular levels were quantified following a recently optimized protocol by Bonet-Aleta et al 32 (see Experimental section). Both cancer cell lines ( i.e.…”

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Cation Leaching In the Presence Of Gshmentioning

confidence: 99%

mentioning

confidence: 99%

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Unveiling the interplay between homogeneous and heterogeneous catalytic mechanisms in copper–iron nanoparticles working under chemically relevant tumour conditions

et al. 2022

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Core@paratroopers Nanoassemblies with Catalytic Cascade for Efficient Tumor Starvation Therapy

Lu,

Hou,

Huang

et al. 2024

Adv Funct Materials

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The catalytic therapy based on the nanozymes has received increasing interest in cancer treatment. However, the catalytic capabilities of standalone nanozymes are relatively limited, necessitating the development of a nano‐bio composite system that integrates both nanozymes and natural enzymes. This construction often inevitably leads to interference between natural enzyme and nanozymes, resulting in reduced synergistic performance. Herein, a cascade catalysis system featuring the “core@paratroopers” structure is proposed, wherein hollow manganese dioxide (HMnO2) serves as “core” and ultra‐small hybrid single‐micelle (H‐micelle) encapsulated with glucose oxidase (GOx) as “paratroopers” (H‐micelle‐GOx). The outer SiO2 layer of the H‐micelle can effectively protect the GOx. Under hypoxic conditions, HMnO2 reacts with endogenous H2O2 to produce O2, thereby enhancing the catalytic efficiency of GOx for starvation therapy. Simultaneously, the generated H2O2 boosts the catalytic efficiency of HMnO2, accelerating local O2 generation and alleviating tumor hypoxia. Additionally, this system exhibits rapid degradation in the tumor microenvironment characterized by high glutathione (GSH) expression, facilitating the release and deep penetration of the ultra‐small H‐micelle‐GOx “paratroopers” within the solid tumor.

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Enhancing Pt(IV) Complexes' Anticancer Activity upon Encapsulation in Stimuli‐Responsive Nanocages

Sancho‐Albero

Facchetti

Panini

et al. 2023

Adv Healthcare Materials

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Platinum-based chemotherapy is the first-line treatment for different cancer types and in particular for malignant pleural mesothelioma patients (a tumor histotype with urgent medical needs). Herein we present a strategy to stabilize, transport and intracellular release of a platinum IV (Pt IV ) prodrug using a breakable nanocarrier. Its reduction, and therefore activation as anticancer drug, is promoted by the presence of glutathione in neoplastic cells that also causes the destruction of the carrier. The nanocage presents a single internal cavity in which the hydrophobic complex (Pt(dach)Cl2(OH)2), (dach= R,R-diaminocyclohexane) has been encapsulated. We have evaluated the in vitro uptake and the internalization kinetics in cancer model cells and using flow cytometry analysis, demonstrated the successful release and activation of the Pt based drug inside cancer cells. The in vitro findings were confirmed by the in vivo experiments on a mice model obtained by xenografting MPM487, a patientderived malignant pleural mesothelioma. MPM487 confirmed the well-known resistance of malignant pleural mesothelioma to cisplatin treatment while an interesting 50% reduction of tumor growth was observed when mice were treated with the Pt IV , entrapped in the nanocages, at an equivalent dose of platinum complex.Received: ((will be filled in by the editorial staff))Revised: ((will be filled in by the editorial staff))

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Glutathione-Triggered catalytic response of Copper-Iron mixed oxide Nanoparticles. Leveraging tumor microenvironment conditions for chemodynamic therapy

Cited by 36 publications

References 78 publications

Unveiling the interplay between homogeneous and heterogeneous catalytic mechanisms in copper–iron nanoparticles working under chemically relevant tumour conditions

Unveiling the interplay between homogeneous and heterogeneous catalytic mechanisms in copper–iron nanoparticles working under chemically relevant tumour conditions

Core@paratroopers Nanoassemblies with Catalytic Cascade for Efficient Tumor Starvation Therapy

Enhancing Pt(IV) Complexes' Anticancer Activity upon Encapsulation in Stimuli‐Responsive Nanocages

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