GlutathioneS-Transferases of Female A/J Mouse Liver and Forestomach and Their Differential Induction by Anti-carcinogenic Organosulfides from Garlic

Hu, Xun; Benson, Patrick J.; Srivastava, Sanjay; Mack, L. M.; Xia, Hong; Gupta, V. D.; Zaren, Howard A.; Singh, Shivendra V.

doi:10.1006/abbi.1996.0550

Cited by 84 publications

(66 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For instance, the naturally occurring OSC analogues are highly effective in affording protection against benzo(a)pyrene-induced forestomach and pulmonary carcinogenesis in mice (8), N-nitrosomethylbenzylamine-induced esophageal cancer in rats (9), azoxymethane-induced colonic aberrant crypt foci formation in rats (10), and N-methyl-N-nitrosoureainduced mammary carcinogenesis in rats (11). The OSCs are believed to inhibit chemically induced cancers by increasing the expression of phase 2 carcinogen-inactivating enzymes, including glutathione (GSH) transferases and quinone reductase, and/or by inhibiting cytochrome P450-dependent monooxygenases (12)(13)(14). We have also shown previously that oral administration of DADS significantly inhibits growth of H-ras oncogene-transformed tumor xenografts in athymic mice without causing weight loss or any other side effects (15).…”

Section: Introductionmentioning

confidence: 99%

c-Jun NH2-Terminal Kinase Signaling Axis Regulates Diallyl Trisulfide–Induced Generation of Reactive Oxygen Species and Cell Cycle Arrest in Human Prostate Cancer Cells

et al. 2006

Self Cite

View full text Add to dashboard Cite

We have shown previously that generation of reactive oxygen species (ROS) is a critical event in G 2 -M phase cell cycle arrest caused by diallyl trisulfide (DATS), which is a highly promising anticancer constituent of processed garlic. Using DU145 and PC-3 human prostate cancer cells as a model, we now report a novel mechanism involving c-Jun NH 2 -terminal kinase (JNK) signaling axis, which is known for its role in regulation of cell survival and apoptosis, in DATS-induced ROS production. The DATS-induced ROS generation, G 2 -M phase cell cycle arrest and degradation, and hyperphosphorylation of Cdc25C were significantly attenuated in the presence of EUK134, a combined mimetic of superoxide dismutase and catalase. Interestingly, the DATS-induced ROS generation and G 2 -M phase cell cycle arrest were also inhibited significantly in the presence of desferrioxamine, an iron chelator, but this protection was not observed with iron-saturated desferrioxamine. DATS treatment caused a marked increase in the level of labile iron that was accompanied by degradation of light chain of iron storage protein ferritin. Interestingly, DATSmediated degradation of ferritin, increase in labile iron pool, ROS generation, and/or cell cycle arrest were significantly attenuated by ectopic expression of a catalytically inactive mutant of JNK kinase 2 and RNA interference of stressactivated protein kinase/extracellular signal-regulated kinase 1 (SEK1), upstream kinases in JNK signal transduction pathway. In conclusion, the present study provides experimental evidence to indicate existence of a novel pathway involving JNK signaling axis in regulation of DATS-induced ROS generation.

show abstract

Section: Introductionmentioning

confidence: 99%

c-Jun NH2-Terminal Kinase Signaling Axis Regulates Diallyl Trisulfide–Induced Generation of Reactive Oxygen Species and Cell Cycle Arrest in Human Prostate Cancer Cells

et al. 2006

Self Cite

View full text Add to dashboard Cite

show abstract

“…The effects of curcumin feeding on the levels of hepatic and forestomach GSTs were determined by using a protocol described by us previously (27), which involves GSH-affinity chromatography followed by reverse-phase HPLC. Briefly, equal amounts (0.25 g) of liver or forestomach tissues from control and curcumin-fed mice were homogenized in 10 mM potassium phosphate buffer, pH 7.0, containing 1.4 mM 2-mercaptoethanol.…”

Section: Effects Of Curcumin Feeding On the Levels Of Hepatic And Formentioning

confidence: 99%

“…The GST isoenzymes were eluted with a 30-min linear gradient of 39-69% solvent II at a column flow rate of 1 ml/min. The GST subunits were identified on the basis of their elution profiles during reverse-phase HPLC analysis (27). Each GST subunit was quantitated by using standard curve for the respective subunit (27).…”

Section: Effects Of Curcumin Feeding On the Levels Of Hepatic And Formentioning

confidence: 99%

Mechanism of inhibition of benzo[a]pyrene-induced forestomach cancer in mice by dietary curcumin

et al. 1998

View full text Add to dashboard Cite

Curcumin (diferuloylmethane), the major yellow pigment in turmeric, has been shown to inhibit benzo[a]pyrene (BaP)-induced forestomach cancer in mice through mechanism(s) not fully understood. It is well known that while cytochrome P4501A1 (CYP1A1) and epoxide hydrolase (EH) are important in the conversion of BaP to its activated form, (⍣)-anti-7,8-dihydroxy-9,10-oxy-7,8,9,10-tetrahydrobenzo[a]pyrene [(⍣)-anti-BaPDE], the detoxification of (⍣)-anti-BaPDE is accomplished by glutathione (GSH) S-transferases (GST). Therefore, it seems reasonable to postulate that curcumin may exert anti-carcinogenic activity either by inhibiting activation of BaP or (and) by enhancing the detoxification of (⍣)-anti-BaPDE. Administration p.o. of 2% curcumin in the diet to female A/J mice for 14 days, which has been shown to cause a significant inhibition in BaP-induced forestomach tumorigenesis, resulted in a modest but statistically significant reduction in hepatic ethoxyresorufin O-deethylase (EROD) activity, a reaction preferentially catalyzed by CYP1A1. While EROD activity could not be detected in the forestomach of either control or treated mice, curcumin feeding caused a statistically significant increase (~2.3-fold) in hepatic EH and GST activities. Hepatic and forestomach GSH levels, and forestomach EH and GST activities were not affected by curcumin treatment. Even though the levels of various hepatic GST isoenzymes were significantly increased upon curcumin feeding, maximum induction was noticed for the pi class isoenzyme (mGSTP1-1), which among murine hepatic GSTs is highly efficient in the detoxification of (⍣)-anti-BaPDE. In conclusion, the results of the present study suggest that curcumin may inhibit BaP-induced forestomach cancer in mice by affecting both activation as well as inactivation pathways of BaP metabolism in the liver.

show abstract

“…GSTs are important detoxifying enzymes, which stimulate the clearance of reactive compounds by conjugation with GSH. The group of Singh has shown that the chemopreventive effect of DADS, against benzo[a]pyrene-induced forestomach cancer in mice, was mainly mediated through the induction of the pi class mGSTP1-1 in liver and forestomach (Bose et al, 2002;Hu et al, 1996). However, different isoenzyme profiles could be obtained according to the target tissue considered.…”

Section: Modulation Of the Metabolism Of Carcinogensmentioning

confidence: 99%

Naturally Occurring Organic Sulfur Compounds: An Example of a Multitasking Class of Phytochemicals in Anti-Cancer Research

Cerella¹,

Kelkel²,

Viry³

et al. 2011

Phytochemicals - Bioactivities and Impact on Health

View full text Add to dashboard Cite

GlutathioneS-Transferases of Female A/J Mouse Liver and Forestomach and Their Differential Induction by Anti-carcinogenic Organosulfides from Garlic

Cited by 84 publications

References 0 publications

c-Jun NH2-Terminal Kinase Signaling Axis Regulates Diallyl Trisulfide–Induced Generation of Reactive Oxygen Species and Cell Cycle Arrest in Human Prostate Cancer Cells

c-Jun NH2-Terminal Kinase Signaling Axis Regulates Diallyl Trisulfide–Induced Generation of Reactive Oxygen Species and Cell Cycle Arrest in Human Prostate Cancer Cells

Mechanism of inhibition of benzo[a]pyrene-induced forestomach cancer in mice by dietary curcumin

Naturally Occurring Organic Sulfur Compounds: An Example of a Multitasking Class of Phytochemicals in Anti-Cancer Research

Contact Info

Product

Resources

About