Glycation-assisted synthesized gold nanoparticles inhibit growth of bone cancer cells

Rahim, Moniba; Iram, Sana; Khan, Mohd Sajid; Khan, M. Salman; Shukla, Ankur R.; Srivastava, Arti; Ahmad, Saheem

doi:10.1016/j.colsurfb.2013.12.008

Cited by 46 publications

(34 citation statements)

References 42 publications

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“…There are also couple of recent reports for the presence of the auto-antibodies in the sera of the cancer patients which needs to be validated further [37]. Therefore the need of the hour is to stop this dreaded reaction using novel approach like nanoconjugation [38], [39]. Our research group has recently shown some medicinal plants that have potent antioxidant property like Phyllanthus virgatus , Ficus virens and Boerhaavia diffusa [40]–[42].…”

Section: Discussionmentioning

confidence: 99%

An Immunohistochemical Analysis to Validate the Rationale behind the Enhanced Immunogenicity of D-Ribosylated Low Density Lipo-Protein

et al. 2014

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Advanced glycation end products (AGEs) are thought to contribute to the abnormal lipoprotein profiles and increased risk of cardiovascular disease in patients with diabetes and renal failure. D-ribose is one of the naturally occurring pentose monosaccharide present in all living cells and is a key component of numerous biomolecules involved in many important metabolic pathways. Formation of D-ribose derived glycated low density lipoprotein (LDL) has been previously demonstrated but no studies have been performed to assess the immune complex deposition in the kidney of rabbits immunized with glycated LDL. In this study, LDL was glycated with D-ribose, and it was further used as an immunogen for immunizing NZW female rabbits. The results showed that female rabbits immunized with D-ribose modified LDL induced antibodies as detected by direct binding and competitive ELISA. The modified LDL was found to be highly immunogenic eliciting high titer immunogen-specific antibodies, while the native forms were moderately immunogenic. The induced antibodies from modified LDL exhibited wide range of heterogeneity in recognizing various proteins and amino acids conformers. Furthermore, our histopathological results illustrated the deposits of immune complex in glomerular basement membrane in rabbits immunized with D-ribose-LDL.

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Section: Discussionmentioning

confidence: 99%

An Immunohistochemical Analysis to Validate the Rationale behind the Enhanced Immunogenicity of D-Ribosylated Low Density Lipo-Protein

et al. 2014

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“…Furthermore, it has also been found recently that the threat of the glycation reaction can be stopped using metformin and pyridoxamine which are excellent anti‐oxidants as well . Last but not the least, we have just reported that glycation‐assisted synthesized gold‐nanoparticles inhibited the formation of bone cancer cells . Therefore, implying alternative approach to the stop the disease progression is of vital importance.…”

Section: Resultsmentioning

confidence: 94%

Immuno‐chemistry of hydroxyl radical modified GAD‐65: A possible role in experimental and human diabetes mellitus

et al. 2015

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The repertoire of known auto-antigens is limited to a very small proportion of all human proteins, and the reason why only some proteins become auto-antigens is unclear. The 65 kDa isoform of the enzyme glutamic acid decarboxylase (GAD-65) is a major auto-antigen in type I diabetes, and in various neurological diseases. Most patients with type I diabetes (70-80%) have auto-antibodies against GAD-65, which often appear years before clinical onset of the autoimmune diabetes. Thus, the aim of the study is to focus on the immunogenicity of GAD65 and its reactive oxygen species (ROS) conformer in STZ-induced diabetic rats and on human diabetic patients. In the present study, GAD-65 was modified by hydroxyl radical following Fenton's reaction. The modifications in the structure of the GAD-65 are supported by UV-vis and fluorescence spectral studies. Immunogenicity of both native and hydroxyl radical modified GAD-65 (ROS-GAD-65) was studied in experimental rabbits and was confirmed by inducing type I diabetes in experimental male albino rats using streptozotocin (45 mg/kg). We found that ROS-GAD-65 was a better immunogen as compared to the native GAD-65. A considerable high binding to ROS-GAD-65 was observed as compared to native GAD-65 in both the serum antibodies from diabetes animal models and as well as in the serum samples of type I diabetes. Hydrogen peroxide under the exposure of UV light produces hydroxyl radical (ÁOH) which is most potent oxidant, and could cause protein damage (GAD-65) to the extent of generating neo-epitopes on the molecule, thus making it immunogenic. V C 2015 IUBMB Life, 67(10): [746][747][748][749][750][751][752][753][754][755][756] 2015

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“…Therefore, need of the hour is to synthesize potential drugs which are peptide/protein specific and should have least or no toxicity to the nearby cells or tissues [53]. Our research group has also done recent developments in the inhibition of bone cancer cells using glycation assisted synthesized gold nanoparticles [54].…”

Section: Conclusion and Future Prospectivementioning

confidence: 99%

Protein Glycation: A Firm Link to Cause Metabolic Disease and their Complications

Siddiqui¹

2015

J Glycomics Lipidomics

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Glycation of proteins starts with the formation of Schiff base, followed by intermolecular rearrangement and conversion into Amadori products. When large amounts of Amadori products are formed, they undergo cross linkage to form a heterogeneous group of protein-bound moieties, termed as Advanced Glycation End products (AGEs). The formation of AGEs is irreversible process, causing structural and functional changes in protein. This results in generation of free radicals which play an important role in pathophysiology of ageing and diabetes. The rates of these reactions are quite slow and proteins with large amounts of lysine residues undergo glycation with significant amounts of AGEs. The unwanted consequences of protein glycation may lead to several metabolic disorders like diabetes, arteriosclerosis, osteoporosis and Alzheimer's disease etc. and their complications. This commentary reviews the glycation of proteins which have already been demonstrated by us and others.

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Glycation-assisted synthesized gold nanoparticles inhibit growth of bone cancer cells

Cited by 46 publications

References 42 publications

An Immunohistochemical Analysis to Validate the Rationale behind the Enhanced Immunogenicity of D-Ribosylated Low Density Lipo-Protein

An Immunohistochemical Analysis to Validate the Rationale behind the Enhanced Immunogenicity of D-Ribosylated Low Density Lipo-Protein

Immuno‐chemistry of hydroxyl radical modified GAD‐65: A possible role in experimental and human diabetes mellitus

Protein Glycation: A Firm Link to Cause Metabolic Disease and their Complications

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