Glycerol Induced Hemoglobinuric Acute Renal Failure in the Rat

Abstract

Hemoglobinuric acute renal failure occurs consistently in rats deprived of water for 24 h and injected with 50% glycerol solution (10 ml/kg) intramuscularly. Severe azotemia developed in 33% of non-dehydrated rats receiving glycerol but 44% were not azotemic, thus permitting a study of the factors which predispose to the development of acute renal failure. There was no necessary correlation between urine volume, urine osmolality, or plasma volume before injection and the development of oliguric acute renal failure, nor was the urine pH during hemoglobinuria correlated with the subsequent severity of azotemia. Plasma volume depletion was the only major difference detected between dehydrated rats which universally developed severe acute renal failure and non-dehydrated rats which were frequently spared. Small differences in plasma volume did not appear to alter the severity of renal involvement in non-dehydrated rats, however.

Cardiovascular responses in rats with glycerol‐induced acute renal failure

“…Acute renal failure was produced by intramuscular injection of glycerol (Thiel, Wilson, Arce & Oken, 1967). Male Wistar albino rats (250-350g) were deprived of drinking water for 24 h but allowed food ad lib.…”

A New Renal Mitochondrial Carrier, KMCP1, Is Up-regulated during Tubular Cell Regeneration and Induction of Antioxidant Enzymes

“…This recovery phase includes cell dedifferentiation and proliferation to restore cell number, followed by redifferentiation, which ultimately results in the restoration of the structural and functional integrity of the tubular epithelium (22). In our mouse model of glycerol-mediated renal failure, all of these processes were observed sequentially: only hours after the myoglobinuric state induced by glycerol injection (23), acute renal failure occurred as reflected by rising blood urea nitrogen and creatinine levels. Interestingly, histological examination of kidney sections and caspase-3 levels as well as TUNEL experiments showed apoptosis and necrosis occurring predominantly in renal proximal tubular cells only a few hours after glycerol challenge.…”

Dibromosulphophthalein: its pharmacokinetics and binding to hepatic cytosol proteins in rats with acute renal failure

“…Induction of renalfailure ARF was induced according to the method developed by Thiel et al (1967). Male Wistar albino rats (240-320g) were dehydrated for 24h and ARF was produced by intramuscular injection of 50% v/v glycerol in sterile saline (0.9% w/v NaCl), l0mikg-1.…”