Glycerol Metabolism of &lt;i&gt;Lactobacillus rhamnosus&lt;/i&gt; ATCC 7469: Cloning and Expression of Two Glycerol Kinase Genes

Álvarez, María de Fátima; Medina, Roxana Beatriz; Pasteris, Sergio E.; Saad, A. M. Strasser De; Sesma, Fernando

doi:10.1159/000079826

Cited by 29 publications

(7 citation statements)

References 64 publications

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“…DHAP is an intermediate in glycerol metabolism and was found to increase by 3.3-fold in MOM samples (p < 0.001). It has been reported that several bacterial species found in milk, including Lactobacillus plantarum, L. delbrueckii, L. acidophilus, L. rhamnosus, and Enterococcus faecalis, are able to catabolize glycerol into DHAP through their metabolism [48,49]. It has also been reported that gut microbiota can use choline as a precursor to produce phosphatidylcholine [50,51].…”

Section: Milk Personalization Results In Global Shifts In the Metabolmentioning

confidence: 99%

Metabolomic Profile of Personalized Donor Human Milk

et al. 2020

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Human milk could be considered an active and complex mixture of beneficial bacteria and bioactive compounds. Since pasteurization drastically reduces the microbial content, we recently demonstrated that pasteurized donor human milk (DHM) could be inoculated with different percentages (10% and 30%) of mother’s own milk (MOM) to restore the unique live microbiota, resulting in personalized milk (RM10 and RM30, respectively). Pasteurization affects not only the survival of the microbiota but also the concentration of proteins and metabolites, in this study, we performed a comparative metabolomic analysis of the RM10, RM30, MOM and DHM samples to evaluate the impact of microbial restoration on metabolite profiles, where metabolite profiles clustered into four well-defined groups. Comparative analyses of DHM and MOM metabolomes determined that over one thousand features were significantly different. In addition, significant changes in the metabolite concentrations were observed in MOM and RM30 samples after four hours of incubation, while the concentration of metabolites in DHM remained constant, indicating that these changes are related to the microbial expansion. In summary, our analyses indicate that the metabolite profiles of DHM are significantly different from that of MOM, and the profile of MOM may be partially restored in DHM through microbial expansion.

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Section: Milk Personalization Results In Global Shifts In the Metabolmentioning

confidence: 99%

Metabolomic Profile of Personalized Donor Human Milk

et al. 2020

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“…H. volcanii (and some other haloarchaea) (Rawls et al, 2011;Williams et al, 2017). The presence of two GK paralogues appears even more unusual and has been described so far for Lactobacillus rhamnosus and Pseudomonas aeruginosa (de Fátima Alvarez et al, 2004;Tang et al, 2023). In the archaeal domain, also few haloarchaea were found to harbor two GK copies (Williams et al, 2017).…”

Section: Only Saci_2033 Gk Is Essential For Glycerol Degradationmentioning

confidence: 76%

Glycerol degradation in the thermoacidophilic crenarchaeonSulfolobus acidocaldariusinvolves an unusual glycerol-3-phosphate dehydrogenase

Schmerling,

Schroeder,

Zhou

et al. 2024

Preprint

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Glycerol is highly abundant in nature and serve as carbon source for many organisms. Also, several Archaea have the genetic capacity to grow on glycerol but its degradation has so far only been studiedHaloferax volcanii.Herein, the thermoacidophilic crenarchaeonSulfolobus acidocaldariuswas shown to grow with glycerol as sole carbon and energy source. After uptake likely involving facilitated diffusion, glycerol is degraded via phosphorylation to glycerol-3-phosphate followed by oxidation to dihydroxyacetone phosphate (DHAP) catalyzed by glycerol kinase (GK) by an unusual quinone reducing FAD-dependent glycerol-3-phosphate dehydrogenase (G3PDH), respectively. TheS. acidocaldariusgenome harbors two paralogous copies of each GK and G3PDH. However, only one of these GK-G3PDH couples (Saci_2031-2033) is highly upregulated on glycerol. Deletion of thesaci_2033gene encoding GK abolished growth on glycerol and GK activity in crude extracts. In contrast, deletion of the second GK gene (saci_1117) had only minor effects indicating that only one of the two GK-G3PDH couples is essential. Biochemical characterization revealed that both isoenzymes of each, GK and G3PDH, were functionally similar. Whereas the GKs showed high similarity to known enzymes from Bacteria and Eukaryotes, the G3PDHs represent unusual homologues of the bacterial GlpA subunit of the GlpABC complex with remarkable C-terminal sequence differences and a novel type of membrane anchoring via a CoxG-like protein (Saci_2031). Further sequence analyzes discovered a higher versatility of G3PDHs in Archaea with respect to interacting proteins, electron transfer, and membrane anchoring likely reflecting tailored evolutionary solutions to meet different requirements caused by life styles and electron acceptors.

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“…The quantification of H 2 O 2 production by S. pluranimalium 2N12 was assessed using the LAPTg medium described by Alvarez et al ( 14 ). LAPTg medium contains 1.5% (w/v) peptone, 1% (w/v) tryptone, 1% (w/v) yeast extract, 1% (w/v) glucose, and 0.1% (v/v) Tween 80.…”

Section: Methodsmentioning

confidence: 99%

Streptococcus pluranimalium 2N12 Exerts an Antagonistic Effect Against the Swine Pathogen Actinobacillus pleuropneumoniae by Producing Hydrogen Peroxide

et al. 2021

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Actinobacillus pleuropneumoniae is the causal agent of porcine pleuropneumonia, a highly contagious and often deadly respiratory disease that causes major economic losses in the swine industry worldwide. The aim of the present study was to investigate the hydrogen peroxide (H2O2)-dependent antagonistic activity of Streptococcus pluranimalium 2N12 (pig nasal isolate) against A. pleuropneumoniae. A fluorimetric assay showed that S. pluranimalium produces H2O2 dose- and time-dependently. The production of H2O2 increased in the presence of exogenous lactate, suggesting the involvement of lactate oxidase. All 20 strains of A. pleuropneumoniae tested, belonging to 18 different serovars, were susceptible to H2O2, with minimal inhibitory concentrations and minimal bactericidal concentrations ranging from 0.57 to 2.3 mM. H2O2, as well as a culture supernatant of S. pluranimalium, killed planktonic cells of A. pleuropneumoniae. Treating the culture supernatant with catalase abolished its bactericidal property. H2O2 was also active against a pre-formed biofilm-like structure of A. pleuropneumoniae albeit to a lesser extent. A checkerboard assay was used to show that there were antibacterial synergistic interactions between H2O2 and conventional antibiotics, more particularly ceftiofur. Based on our results and within the limitations of this in vitro study, the production of H2O2 by S. pluranimalium could be regarded as a potential protective mechanism of the upper respiratory tract against H2O2-sensitive pathogens such as A. pleuropneumoniae.

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Glycerol Metabolism of <i>Lactobacillus rhamnosus</i> ATCC 7469: Cloning and Expression of Two Glycerol Kinase Genes

Cited by 29 publications

References 64 publications

Metabolomic Profile of Personalized Donor Human Milk

Metabolomic Profile of Personalized Donor Human Milk

Glycerol degradation in the thermoacidophilic crenarchaeonSulfolobus acidocaldariusinvolves an unusual glycerol-3-phosphate dehydrogenase

Streptococcus pluranimalium 2N12 Exerts an Antagonistic Effect Against the Swine Pathogen Actinobacillus pleuropneumoniae by Producing Hydrogen Peroxide

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