Glycerol Waste to Bio-Ethanol: Optimization of Fermentation Parameters by the Taguchi Method

Chilakamarry, Chaitanya Reddy; Sakinah, A. M. Mimi; Zularism, A. W.; Khilji, Irshad Ahamad; Sudhakar, K.

doi:10.1155/2022/4892992

Cited by 12 publications

(6 citation statements)

References 40 publications

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“…The actual R 2 (0.921) and adjusted R 2 (0.915) are in reasonable agreement. It is evident that there is a good agreement between the experimental and the theoretical values predicted by the model and almost all the variations could be accounted for by the equation of the model [40].…”

Section: Optimum Fermentation Conditions For Bio-ethanol Productionmentioning

confidence: 54%

Optimization of parameters for bio-ethanol production from sweet sorghum (Sorghum bicolor (L.) Moench) stalk juice and finger millet malt using Taguchi method

Okoth,

Otieno,

Kiema

et al. 2023

Bull. Chem. Soc. Eth.

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Bio-ethanol is a promising renewable energy but its production is expensive from high cost of feedstocks. In this study, sweet sorghum (Sorghum bicolor (L.) Moench) stalk juice was investigated for bio-ethanol production. Most reports on bio-ethanol productions use commercial Saccharomyces cerevisiae as yeast. However, this study used finger millet (Eleusine coracana) malt with already high adaptation to local conditions and high economic viability as it is being utilized by the indigenous communities. Five sweet sorghum varieties of IESV-92001-DL (V1), NTJ (V2), 15233-IESV (V3), 92008-DJ (V4) and IESV-92028-DL (V5) were planted at 0°3'45.4644" North, 34°17'16.1052" South, in Kenya. °Brix content of juice was determined at 11th to 16th weeks after sowing. Highest °Brix for all varieties were at 15th week where V1 was highest at 22.07. V1 was then harvested for the juice. Factors affecting fermentation; temperature, time, pH and yeast to substrate ratio were optimized using Taguchi method and were obtained as 30 ℃, 48 hours, pH 5 and 5 g/L, respectively. Kinetics parameters of Vmax and Km were 0.35 g/L/h and 12.56 g/L, respectively. The optimized and kinetic parameters were within literature values and therefore finger millet malt has a great potential, as a substitute yeast source, in commercial bio-ethanol production. KEY WORDS: Bio-ethanol, Sweet sorghum juice, Taguchi method Bull. Chem. Soc. Ethiop. 2024, 38(1), 55-67. DOI: https://dx.doi.org/10.4314/bcse.v38i1.5

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Section: Optimum Fermentation Conditions For Bio-ethanol Productionmentioning

confidence: 54%

Optimization of parameters for bio-ethanol production from sweet sorghum (Sorghum bicolor (L.) Moench) stalk juice and finger millet malt using Taguchi method

Okoth,

Otieno,

Kiema

et al. 2023

Bull. Chem. Soc. Eth.

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“…So cheap fermentation and seed media for 1,3-PDO production were investigated for phage preparation in this study. The infectivity of phiKpS2 incubated in the fermentation medium containing 40 g/L glycerol and seed medium with 5-20 g/L glycerol were compared in this study, which were usual concentrations for glycerol fermentation and seed cultivation (Chilakamarry et al 2022;Unrean et al 2012;Zhang et al 2007). Bacterial pellet precipitates used in this process were obtained from the first fed-batch fermentation, during which the biomass (OD 650 ) in the fermentation broth remained at a high level, and the 1,3-PDO concentration was relatively low (Fig.…”

Section: Condition Optimization Of Phage Infection To Bacterial Pelle...mentioning

confidence: 99%

Co-production of 1,3-propanediol and phage phiKpS2 from the glycerol fermentation by Klebsiella pneumoniae

Duan,

Zhang,

Wang

et al. 2024

Bioresour. Bioprocess.

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As an alternative to antibiotics in response to antimicrobial-resistant infections, bacteriophages (phages) are garnering renewed interest in recent years. However, the massive preparation of phage is restricted using traditional pathogens as host cells, which incurs additional costs and contamination. In this study, an opportunistic pathogen, Klebsiella pneumoniae used to convert glycerol to 1,3-propanediol (1,3-PDO), was reused to prepare phage after fermentation. The phage infection showed that the fed-batch fermentation broth containing 71.6 g/L 1,3-PDO can be directly used for preparation of phage with a titer of 1 × 108 pfu/mL. Then, the two-step salting-out extraction was adopted to remove most impurities, e.g. acetic acid (93.5%), ethanol (91.5%) and cells (99.4%) at the first step, and obtain 1,3-PDO (56.6%) in the top phase as well as phage (97.4%) in the middle phase at the second step. This integrated process provides a cheap and environment-friendly manner for coproduction of 1,3-PDO and phage. Graphical abstract

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“…This vibration band was partially superposed by those related to glycerol, whose presence in the films is indicated by the band of C O bonds in secondary alcohols at 1110 cm À1 , the band at 1040 cm À1 associated with C O stretching of primary alcohols and the C O H stretching at 923 cm À1 . 49,50 The degree of crystallinity and secondary structure fractions were determined from the XRD and FTIR data using the deconvolution method. The results were summarized in Table S1, and the curves can be found in Figures S3 and S4.…”

Section: Characterization Of Keratin Filmsmentioning

confidence: 99%

Effect of precipitation methods on physicochemical properties of keratin films produced from chicken feather waste

Vanderlei,

Novo‐Mansur,

Mattoso

et al. 2024

J of Applied Polymer Sci

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Engineering keratin plastics from chicken feather wastes is highly attractive from an environmental prospect but challenging due to the influence of extraction routes on the macromolecular keratin features. In this study, the effect of precipitation methods on desired properties of biodegradable keratin films was investigated. Keratin was extracted from an industrial chicken feather waste by sulfitolysis, and further recovered using hydrochloric acid (KHA), citric acid (KCA), sodium chloride (KSC), and acetone (KAT) as precipitating agents. Keratin films containing glycerol at 25 wt% were produced by compression molding. All keratin films were nearly colorless and highly transparent to visible light while exhibiting good UV barrier properties. The KAT and KSC films showed greater ductility (εbreak > 50%) compared with KHA and KCA, which was correlated with broader molecular weight distributions and less crosslinking in the former keratins. Importantly, disulfide bonds created in the processing led to keratin films with low water solubility. Larger surface hydrophobicity was attained from keratin recovered by organic solvent precipitation (KAT). These results advance the understanding of precipitating agents as physicochemical property modifiers of biodegradable keratin films for food packaging and other applications.

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Glycerol Waste to Bio-Ethanol: Optimization of Fermentation Parameters by the Taguchi Method

Cited by 12 publications

References 40 publications

Optimization of parameters for bio-ethanol production from sweet sorghum (Sorghum bicolor (L.) Moench) stalk juice and finger millet malt using Taguchi method

Optimization of parameters for bio-ethanol production from sweet sorghum (Sorghum bicolor (L.) Moench) stalk juice and finger millet malt using Taguchi method

Co-production of 1,3-propanediol and phage phiKpS2 from the glycerol fermentation by Klebsiella pneumoniae

Effect of precipitation methods on physicochemical properties of keratin films produced from chicken feather waste

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