GLYCINE RESISTANCE IN AGROBACTERIUM TUMEFACIENS

Abstract

B3EARDSLEY, ROBERT E. (MIanhattan College,-New York, N. Y.). Glycine resistance in Agrobacterinllm tumefaciens. J. Bacteriol. 83:6-13. 1962.-The application of the fluctuation test of Luria and Delbriuck to the distribution of glycine-resistant bacteria among cultures of Agrobacterium tumefaciens strain B6 indicates

Physiological and Biochemical Studies on Virulent and Avirulent Isolates of Agrobacterium tumefaciens in Egypt

“…Such observation confirm the findings reached by HENDRICKSON et al (1934) who obtained an attenuated culture which had spontaneously emerged among the progeny of an originally virulent culture of A. tumefaciens. However, several investigators , RIKER 1939, STAPP 1951, VAN LANEN et al 1952, BEARDSLEY 1962, MANIGAULT and KURKDJIAN 1970 2" 20 ABO-EL-DAHAB, EL-GOORANI and EL-WAKIL As far as the routine bacteriological tests were concerned, virulent and avirulent isolates of the present work were identical. This confirm the point of view declared by RIKER and HILDEBRANDT (1951).…”

“…However, HENDRICKSON et al (1934) obtained avirulent cultures that appeared spontaneously among the progeny of an original virulent culture of A. tumefaciens. , RIKER (1939), STAPP (1951), VAN LANEN et al (1952), BEARDSLEY (1962), MANIGAULT and KuRKDjiAN (1970), and FAHMY (1975) could attenuate cultures of A. tumefaciens by adding glycine to the media.…”

AMINO ACID CROSS RESISTANCE IN AGROBACTERIUM TUMEFACIENS

“…The toxicity of glycine, DLnorleucine, D-methionine, and DL-valine was additive in all of the combinations tested. The survival of B6 on glycine alone was previously reported (Beardsley, 1962).…”

“…Media and procedures. The media, culture conditions, and experimental procedures employed in this study were previously reported (Beardsley, 1962). Chemicals.…”

“…They reported additive inhibitory effects for the attenuating compounds. Beardsley (1962) reported that glycine selects for resistant mutants of the crown gall organism, most of which are at least partially attenuated. The present study was undertaken to determine whether different attenuating amino acids select for mutations at different specific loci, each with pleiotropic effects on virulence, or whether a common genetic mechanism is involved.…”