Glycoconjugate histochemistry of Xenopus laevis fundic gland with special reference to mucous neck cells during development

Abstract

Mucous neck cells (MNCs) of the fundic gland are phylogenetically thought to have first appeared in amphibians. We studied the origin and differentiation of MNCs in fundic glands of Xenopus laevis. By means of lectin histochemical methods using Griffonia simplicifolia agglutinin-II (GSA-II), MNCs were detected specifically in fundic glands of adult X. laevis. Mucous granules of MNCs were labeled by GSA-II-colloidal gold (CG) staining. Other cells such as surface mucous cells (SMCs), oxynticopeptic cells (OPCs), and endocrine cells did not react to GSA-II. Ulex europaeus agglutinin-I specifically stained OPCs, but not MNCs and SMCs. During the morphogenetic period of the stomach in metamorphosing larvae, GSA-II reactive cells randomly appeared in various portions of the underdeveloped fundic glands and then rapidly localized in the neck portion. At this time, newly appearing mucous granules of MNC type were labeled by GSA-II-CG. Two types of cells intermediate to MNCs and SMCs and intermediate to MNCs and OPCs were observed in the larval gastric region. Cells intermediate to MNCs and OPCs were also found in adults. In these cells, mucous granules of MNC type were labeled by GSA-II-CG, but mucous granules of SMC type and zymogen-like granules did not react to GSA-II. These observations suggest that GSA-II is a useful marker in studying the differentiation of MNCs and their precursors regardless of species differentiation.

Morphological and histochemical variations of mucous and oxynticopeptic cells in the stomach of the seps, Chalcides chalcides

“…Mitotic figures were found among glandular mucous cells. These proliferating cells could be undifferentiated stem cells that might act as a precursor of oxynticopeptic cells or mucous cells as hypothesised by Oinuma et al (1981) in the clawed toad, Xenopus laevis.…”

Differential expression of the TFF-peptides xP1 and xP4 in the gastrointestinal tract of Xenopus laevis

“…Both cell types share certain histochemical features (Suganuma et al 1981) and GSA-II has been described as a specific marker for the mucous granules of MNCs (Oinuma et al 1991). Phylogenetically, MNCs are thought to have first appeared in amphibians and have not been found in piscine and avian species (Oinuma et al 1991). Ontogenetically, MNCs can be clearly identified only after X. laevis has completed metamorphosis (Ishizuya-Oka and Shimozawa 1990).…”

Die Entwicklung der Magendrüsen der Katze 1 (Felis silvestris catus)

“…Graviditatshalfte angegeben (Tuovinen, 1946). Plenk (1938) (Oinuma et al, 1991). Es sind die von Lim (1922) als 'Schleimzellen' und die von Ascoli (1900) (Salenius, 1962), und von ihnen geht nach Ansicht von Lim (1922) und Zimmermann (1925 die Regeneration aller Driisenzellen aus.…”