Differential expression of the TFF-peptides xP1 and xP4 in the gastrointestinal tract of Xenopus laevis

Jagla, Wolfgang; Wiede, Antje; Kölle, Sabine; Hoffmann, Werner

doi:10.1007/s004410050975

Cited by 40 publications

(61 citation statements)

References 29 publications

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“…Tissue extraction of porcine conjunctiva samples was similar to that described previously (Jagla et al 1998); only the extraction buffer was changed to 6 M guanidinium thiocyanate. Methods for subsequent Western blot analysis were as reported in detail (Jagla et al 1998) using the affinity-purified anti-hTFF3-2 antiserum in a 1:500 dilution.…”

Section: Methodsmentioning

confidence: 99%

Localization of TFF3 peptide to porcine conjunctival goblet cells

Jagla

Wiede²,

Hoffmann³

1999

Cell and Tissue Research

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TFF-peptides (formerly P-domain peptides, trefoil factors) form a new family of mucin-associated peptides mainly in the gastrointestinal tract. TFF3 is a typical secretory product of intestinal goblet cells and occurs also in the respiratory tract. Here, polyclonal antisera specific for TFF3 were used in Western blot analysis and immunofluorescence to determine the presence and distribution of TFF3 in the porcine conjunctiva, which is the primary source for ocular mucins. Significant accumulation of TFF3 was detected in conjunctival goblet cells but not in the lacrimal glands. This peptide, together with ocular mucins, may play a role in the rheological function of the tear film.

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Section: Methodsmentioning

confidence: 99%

Localization of TFF3 peptide to porcine conjunctival goblet cells

Jagla

Wiede²,

Hoffmann³

1999

Cell and Tissue Research

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“…However, porcine TFF2 is well known to crystallize as a non-covalently linked dimer [4,46,47]. Generally, a TFF2 dimer is reminiscent to the xP4 peptides from Xenopus laevis stomach containing four TFF domains in tandem [43]. …”

Section: Discussionmentioning

confidence: 99%

“…Western blot analysis after SDS-PAGE, electrophoretic transfer, and fixation with 0.2% glutaraldehyde were performed according to previous reports [24,43]. Western blot analysis after SDS-AgGE has been described previously [42] using either capillary blotting or electrophoretic transfer.…”

Section: Methodsmentioning

confidence: 99%

Porcine Gastric TFF2 is a Mucus Constituent and Differs from Pancreatic TFF2

Stürmer

Müller

Hanisch

et al. 2014

Cell Physiol Biochem

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Background/Aims: TFF2 is a secretory peptide (106 amino acid residues) of the gastric mucosa, the porcine exocrine pancreas as well as immune cells and the CNS. It was the aim of this study to compare gastric and pancreatic TFF2. Methods: TFF2 was purified from the porcine stomach and pancreas, respectively, by size-exclusion and anion-exchange chromatography and then analyzed by Western blots as well as mass spectrometry. Results: Gastric and pancreatic TFF2 differed markedly, i.e. gastric TFF2 was exclusively associated with the high molecular mass mucus fraction, whereas pancreatic TFF2 appeared as a low molecular mass product. Unexpectedly, the latter predominantly formed a non-covalently linked homodimer resistant to boiling SDS. In contrast, gastric TFF2 is an integral mucus constituent predominantly binding to the mucin MUC6 in a non-covalent fashion. Conclusion: The non-covalent interaction of TFF2 with the mucin MUC6 is typical of a "link peptide" which is perfectly suited to assemble and stabilize the laminated structure of gastric mucus and to modulate its rheological properties.

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“…Mucins were stained with a combination of Alcian blue 8GX at pH 2.5 and the PAS reaction as already described [24]. Nuclei were counterstained with Meyer's hematoxylin.…”

Section: General Histology and Immunofluorescencementioning

confidence: 99%

Expression Analysis of Human Salivary Glands by Laser Microdissection: Differences Between Submandibular and Labial Glands

Kouznetsova

Gerlach

Zahl

et al. 2010

Cell Physiol Biochem

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Both the major and minor salivary glands are the sources of saliva, a fluid vital for the maintenance of a healthy oral cavity. Here, the expression profiles of human submandibular (SMG) and labial glands (LG) were compared by RT-PCR analysis of laser microdissected mucous and serous cells, respectively. The focus was on trefoil factor family (TFF) genes, but also other genes encoding secretory proteins (mucins, lysozyme, amylase, statherin, and histatins) or aquaporin 5 were included. Immunofluorescence studies concerning TFF1-3, FCGBP, amylase, and lysozyme are also presented. It was shown that LGs clearly contain serous cells and that these cells differ in their expression profiles from serous SMG cells. Furthermore, all three TFF peptides, together with MUC5B, MUC7, MUC19, and FCGBP, were clearly detectable in mucous acini of both LGs and SMGs. In contrast, lysozyme was differentially expressed in LGs and SMGs. It can be expected that labial saliva may play a particularly important role for protecting the teeth.

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Differential expression of the TFF-peptides xP1 and xP4 in the gastrointestinal tract of Xenopus laevis

Cited by 40 publications

References 29 publications

Localization of TFF3 peptide to porcine conjunctival goblet cells

Localization of TFF3 peptide to porcine conjunctival goblet cells

Porcine Gastric TFF2 is a Mucus Constituent and Differs from Pancreatic TFF2

Expression Analysis of Human Salivary Glands by Laser Microdissection: Differences Between Submandibular and Labial Glands

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