Glycogen Content in the Rat Testis from Postnatal to Adult Ages

Leiderman, B; Mancini, R. E.

doi:10.1210/endo-85-3-607

Cited by 29 publications

(14 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…production of lactate in the absence of glucose in the extracellular milieu, a result that is seemingly surprising, may be the consequence of metabolism of glucose originated from certain aminoacids or alternatively by glycogen metabolism. Noteworthy, and making the possibility that glycogen hydrolysis fuels the glycolytic pathway feasible, histochemical evidence of the presence of glycogen and of glycogen phosphorylase activity in Sertoli cells has been demonstrated (22,41).…”

Section: Discussionmentioning

confidence: 99%

Molecular mechanisms involved in Sertoli cell adaptation to glucose deprivation

Riera

Galardo

Pellizzari

et al. 2009

American Journal of Physiology-Endocrinology and Metabolism

View full text Add to dashboard Cite

cells provide the physical support and the necessary environment for germ cell development. Among the products secreted by Sertoli cells, lactate, the preferred energy substrate for spermatocytes and spermatids, is present. Considering the essential role of lactate on germ cell metabolism, it is supposed that Sertoli cells must ensure its production even in adverse conditions, such as those that would result from a decrease in glucose levels in the extracellular milieu. The aim of the present study was to investigate 1) a possible effect of glucose deprivation on glucose uptake and on the expression of glucose transporters in rat Sertoli cells and 2) the participation of different signal transduction pathways in the above-mentioned regulation. Results obtained show that decreasing glucose levels in Sertoli cell culture medium provokes 1) an increase in glucose uptake accompanied by only a slight decrease in lactate production, 2) an increase in GLUT1 and a decrease in GLUT3 expression, and 3) an activation of AMP-activated protein kinase (AMPK)-, phosphatidylinositol 3-kinase (PI3K)/PKB-, and p38 MAPK-dependent pathways. Additionally, by using specific inhibitors of these pathways, a possible participation of AMPK-and p38MAPK-dependent pathways in the regulation of glucose uptake and GLUT1 expression is shown. These results suggest that Sertoli cells adapt to conditions of glucose deprivation to ensure an adequate lactate concentration in the microenvironment where germ cell development occurs.

show abstract

Section: Discussionmentioning

confidence: 99%

Molecular mechanisms involved in Sertoli cell adaptation to glucose deprivation

Riera

Galardo

Pellizzari

et al. 2009

American Journal of Physiology-Endocrinology and Metabolism

View full text Add to dashboard Cite

show abstract

“…Chemical and cytochemical studies involving rat testis have tended to confirm this finding (Leiderman & Mancini, 1969).…”

Section: Introductionmentioning

confidence: 99%

Glycogen and Related Enzymes in Normal and Cryptorchid Human Testes: A Cytochemical Study

Seilicovich¹,

Lloret²

1973

Reproduction

View full text Add to dashboard Cite

“…Glucose, pyruvate, glutamine, and 3-hydroxybutyrate can satisfy this requirement (17). Despite the need for an energy source, only small amounts of glycogen are stored in Leydig cells (18). Glucose is transported into Leydig cells by glucose transporter 8, which is induced by hCG and insulin-like growth factor-I but repressed by interleukin-1␣, tumor necrosis factor-␣ (TNF-␣), and interferon-␥ (19 -21).…”

mentioning

confidence: 99%