Glycogen-induced intrauterine leucocytosis and its effect on mouse blastocyst implantation in vivo and in vitro

Waites, Gillian T.; Bae, Sang Cheol

doi:10.1530/jrf.0.0660563

Cited by 20 publications

(8 citation statements)

References 11 publications

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“…It has been demonstrated that two days after ovulation high numbers of neutrophils occur in the lumen of a normal uterus. It seems to be an important factor in the adequate preparation of the intrauterine environment for fertilization and implantation of the ovum (1,13,17). In the present results it was demonstrated that a significantly higher per cent of PMNs isolated after ovulation from the uterine lumen have Fc receptors on their surface, and that these cells possess a, greater phagocytic activity in relation to two standard S. aureus strains than the neutrophils isolated from the peripheral blood of the same animals ( Figs.…”

Section: Discussionsupporting

confidence: 59%

The Phagocytic Activity of Polymorphonuclear Leucocytes Isolated from Normal Uterus and that with Experimentally Induced Inflammation in Cows*

Kluciński¹,

Targowski

Miernik‐Degórska³

et al. 1990

Journal of Veterinary Medicine Series A

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The purpose of the study was the determination of the activity of the neutrophils isolated from a physiologically sound (non-inflammed) uterus 2 days after ovulation, and comparison of this activity with that of the polymorphonuclear leucocytes (PMNs) obtained from an uterus which had undergone experimentally induced specific or non-specific inflammatory reaction. The study was carried out on 20 cows between the 2nd and the 8th day after ovulation. Activity of PMNs was determined on the basis of the per cent of these cells with Fc receptors for IgG on their surface, and on the basis of assessment of the phagocytic activity. The Fc receptors were determined by the rosette test. The phagocytic activity of PMNs was determined in relation to two standard strains of S.aureus Smith which were phagocytized by mediation of Fc receptors and S. aureus 305 phagocytized by mediation of the "non-immunological" receptors. The study showed that the inflammatory process in the uterus caused intensive migration of large numbers of PMNs into the lumen. These PMNs have their properties altered: there is a significantly lower per cent of cells with Fc receptors in this cell population and lower Fc-dependent phagocytic activity. A significant rise in the phagocytic activity mediated by non-immunological receptors occurs at the same time in this cell population.

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Section: Discussionsupporting

confidence: 59%

The Phagocytic Activity of Polymorphonuclear Leucocytes Isolated from Normal Uterus and that with Experimentally Induced Inflammation in Cows*

Kluciński¹,

Targowski

Miernik‐Degórska³

et al. 1990

Journal of Veterinary Medicine Series A

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“…A persistent inflammation often results in premature luteolysis and embryonic loss in response to increased PGFza concentrations. The inflammation could also tnterfere directly with the survival of an embryo (Smith et al, 1971;Waites andBell, 1982, Heuer et al, 1993). Proteolytic enzymes, oxygen radicals and other toxic products are released during PMN-phagocytosis.…”

Section: Pathophysiology Of Mating-induced Endometritismentioning

confidence: 99%

Therapeutic considerations for mating-induced endometritis

Troedsson¹

1997

PHK

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SummaryA transient mating-induced endometritis is most likely physiological in the mare, serving to remove excess spermatozoa, seminal plasma, and contaminants from the uterus. However, the inflammatory reaction has to be resolved and inflammatory products cleared from the uterus prior to embryonal descent into the uterine lumen. Mares that fail to clear a semen-induced inflammation from the uterus develop a persistent mating-induced endometritis which may result in infedility. lf susceptibility to persistent mating-induced endometritis is suspected, the mare should be monitored by transrectal ultrasonography for intrauterine fluid accumulation at 6-1 2 hours intervals after breeding. Treatment should be aimed at assisting the uterus to physically clear contaminants and inflammatory products. Post-mating uterine lavage and/or the use of uterotonic drugs are recommended aI 6 12 hours after breeding in mares susceptible to persistent endometritis. Endometritis is a major cause of subfertility in the mare and has a significant economic impact on the equine industry (Dimmock 1939; Collins, 1964;Bain, 1966;Hughes et al., 1966;Ricketts 1975; Gordon and Sarlin, 1978;Kenney, 1978; Doig et al., 1981; de la Conch-Bermejillo and Kennedy, 1982;Traub-Dargatz et al., 7997J. Based on the current understandjng of its etiology and pathophysiology, endometritis should be considered as a multifactorial disease with at least four different causes: (1) sexually transmitted diseases, (2) chronic infectious endometritis, (3) persisting mating-induced endometritis, and (4) endometrosis (chronic degenerative endometritis). Sexually transmitted diseases and chronic infectious endometritis are often diagnosed first after the invading microorganism already has adhered to the endometrial epithelium and started its replication in the uterus. Similar to infectious diseases in other organ systems, infections in the female reproductive tract (sexually transmitted endometritis and chronic infectious endometritis) are most effectively treated with an appropriate antic t o microbial. In contrast to infectious endometritis, matinginduced endometritis has a known starting point and treatment can therefore, be initiated before the infection or inflammation has established itself in the endometrium. This report will review the therapeutic aspects and pathophysiology (as it relates to therapy) of mating-induced endometriti s . Pathophysiology of mating-induced endometritisRegardless of if mares are bred by natural service or by artificial insemination, semen is deposited directly into the uterus. This provides an excellent oppodunity for uterine contamination with bacteria. In addition, recent reporls have shown that soermatozoa without bacterial contamination induce an uterine inflammatory response (Kotilainen et al., 1994; Troedsson, 1995; Troedsson et al., 7995a). RaIher than considering a transient post-mating endometritis as a pathological condition, mating-induced endometritis isPferdeheilkunde 13

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“…The reduced number of newborns per litter can be explained for two of the nine fertile RT-50 mice, which bore unilateral hydrosalpinges. With regard to the other seven mice, whose tubes were apparently normal, other sequelae, such as impairment of tubal transport of eggs (13) or disturbances in blastocyst implantation subsequent to prolonged uterine leukocytosis (17), may be involved. These results suggest that the 50-mg/kg/day dosage, despite its efficiency in terms of chlamydial elimination, does not always act quickly enough; inflammation may thus continue to progress during treatment initiated on D10 p.i., when the inflammation state is already well advanced.…”

mentioning

confidence: 98%

Roxithromycin treatment of mouse chlamydial salpingitis and protective effect on fertility

Zana

Muffat-Joly

Thomas

et al. 1991

Antimicrob Agents Chemother

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We used a mouse model of acute chlamydial salpingitis to evaluate the efficacy of roxithromycin in preventing irreversible inflammatory damage leading to tubal infertility. Female C3H/He mice were genitally inoculated with a human strain of Chlamydia trachomatis and then treated with roxithromycin glutamate subcutaneously. Treatment was initiated either 7 or 10 days postinfection (p.i.) and continued for 7 days at a dosage of 50 or 100 mg/kg of body weight per 24 h. The course of the disease was monitored serologically, bacteriologically, and histologically. At the end of the treatment, the mice were encaged with males and their reproductive capacity was recorded over a 19-week period. The protective effect of roxithromycin was assessed in terms of fertility parameters in comparison with values for noninfected control mice. When treatment was initiated on day 7 p.i. and given in twice-daily 25-mg/kg doses, all the mice remained fertile and the total number of offspring was similar to that of sham-infected mice (17.3 3.3 versus 17.2 + 2.3). When treatment was initiated on day 10 p.i. and given in a single daily dose of 50 or 100 mg/kg, 90 and 70% of the mice, respectively, remained fertile; however, in terms of total offspring, fertility was lower in the group treated with the lower dose (5.6 1.4 versus 13.0 3.8). Roxithromycin was found to be effective against C. trachomatis in the mouse genital tract, but fertility was only partiaHly preserved when the time between infection and treatment was prolonged.

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Glycogen-induced intrauterine leucocytosis and its effect on mouse blastocyst implantation in vivo and in vitro

Cited by 20 publications

References 11 publications

The Phagocytic Activity of Polymorphonuclear Leucocytes Isolated from Normal Uterus and that with Experimentally Induced Inflammation in Cows*

The Phagocytic Activity of Polymorphonuclear Leucocytes Isolated from Normal Uterus and that with Experimentally Induced Inflammation in Cows*

Therapeutic considerations for mating-induced endometritis

Roxithromycin treatment of mouse chlamydial salpingitis and protective effect on fertility

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