The Phagocytic Activity of Polymorphonuclear Leucocytes Isolated from Normal Uterus and that with Experimentally Induced Inflammation in Cows*

Abstract

The purpose of the study was the determination of the activity of the neutrophils isolated from a physiologically sound (non-inflammed) uterus 2 days after ovulation, and comparison of this activity with that of the polymorphonuclear leucocytes (PMNs) obtained from an uterus which had undergone experimentally induced specific or non-specific inflammatory reaction. The study was carried out on 20 cows between the 2nd and the 8th day after ovulation. Activity of PMNs was determined on the basis of the per cent of these cells with Fc receptors for IgG on their surface, and on the basis of assessment of the phagocytic activity. The Fc receptors were determined by the rosette test. The phagocytic activity of PMNs was determined in relation to two standard strains of S.aureus Smith which were phagocytized by mediation of Fc receptors and S. aureus 305 phagocytized by mediation of the "non-immunological" receptors. The study showed that the inflammatory process in the uterus caused intensive migration of large numbers of PMNs into the lumen. These PMNs have their properties altered: there is a significantly lower per cent of cells with Fc receptors in this cell population and lower Fc-dependent phagocytic activity. A significant rise in the phagocytic activity mediated by non-immunological receptors occurs at the same time in this cell population.

Impact of Ovarian and Uterine Conditions on Some Diagnostic Tests Output of Endometritis in Postpartum High‐Yielding Dairy Cows

“…The main goal of the present study was to investigate the effects of ovarian status and uterine condition during the pp period on the outcome of the diagnostic trials of endometritis in high-producing dairy cows. During the puerperium, the uterus of almost all cows is contaminated, and a mild, non-pathological form of endometritis develops (Anderson et al 1985;Klucinski et al 1990) characterized by migration of neutrophil granulocytes and other inflammatory cells into the endometrium and the uterine cavity (Lewis 1997;BonDurant 1999;Sheldon 2004;Sheldon and Dobson 2004). In the present study, PMN% decreased with increased days pp.…”

“…During the puerperium, the uterus of almost all cows is contaminated, and a mild, non‐pathological form of endometritis develops (Anderson et al. 1985; Klucinski et al. 1990) characterized by migration of neutrophil granulocytes and other inflammatory cells into the endometrium and the uterine cavity (Lewis 1997; BonDurant 1999; Sheldon 2004; Sheldon and Dobson 2004).…”

Effects of prepartum oilseed supplements on subclinical endometritis, pro- and anti-inflammatory cytokine transcripts in endometrial cells and postpartum ovarian function in dairy cows

“…In uterine lavage collected from cows 8 h after ovulation, the concentration of PMN was determined to be ,3% of all cells collected (Butt et al 1991). An increased percentage of PMN in the endometrium of cows was also detected between Days 2 and 8 after ovulation (Kluciński et al 1990a(Kluciński et al , 1990b. This can be interpreted as a first cellular defence against pathogens expected to infiltrate the uterus at mating due to the open cervix during oestrus.…”

Phagocytic Activity of Polymorphonuclear Leukocytes Lavaged from the Lungs of Horses with Clinically Diagnosed Chronic Pulmonary Disease

“…T h e purpose of this research was to determine the phagocytic activity of P M N s U.S. Copynght Clearance Center Code Statement: 0931 -184X/94/4107- 0558$10.50/0 in the bronchoalveolar lavage of clinically healthy horses and, subsequently, compare it with the activity of the same cells lavaged from the lungs of horses with clinically diagnosed chronic pulmonary disease (CPD). The phagocytic activity of PMNs was determined in relation to two standard strains of Staphylococcus aureus, S. aureus Smith, which was phagocytized by mediation of Fc receptors, and S. aureus 305, phagocytized by mediation of 'non-immunologic' receptors (TARGOWSKI and NIEMIALTOWSKI, 1986;KLUCINSKI et al, 1990).…”