Glycogen synthase kinase-3 is involved in regulation of ribosome biogenesis in yeast

Yabuki, Yukari; Kodama, Yushi; Katayama, Masako; Sakamoto, Akiko; Kanemaru, Hirofumi; Wan, Kun; Mizuta, Keiko

doi:10.1080/09168451.2014.905183

Cited by 8 publications

(6 citation statements)

References 25 publications

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“…However, the ability of Pho85 to directly phosphorylate the CTD was previously unknown. Rim 11, another new Thr4 CTD kinase identified in our survey, is required for meiosis 36 and ribosome biogenesis 37 . Finally, four new Thr4 CTD kinases, Ssk2, Cmk1, Cmk2, and Sln1 were implicated in response to multiple environmental stresses 38–41 .…”

Section: Resultsmentioning

confidence: 91%

Noncanonical CTD kinases regulate RNA polymerase II in a gene-class-specific manner

et al. 2018

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Phosphorylation of the carboxyl-terminal domain (CTD) of the largest subunit of RNA polymerase II (Pol II) governs stage-specific interactions with different cellular machines. The CTD consists of Y 1 S 2 P 3 T 4 S 5 P 6 S 7 heptad repeats, and sequential phosphorylations of Ser7, Ser5 and Ser2 occur universally across Pol II-transcribed genes. Phosphorylation of Thr4, however, appears to selectively modulate transcription of specific classes of genes. Here, we identify 10 new Thr4 kinases from different kinase structural groups. Irreversible chemical inhibition of the most active Thr4 kinase, Hrr25, reveals a novel role for this kinase in transcription termination of specific class of noncoding snoRNA genes. Genome-wide profiles of Hrr25 reveal a selective enrichment at 3ʹ regions of noncoding genes that display termination defects. Importantly, phospho-Thr4 marks placed by Hrr25 are recognized by Rtt103, a key component of the termination machinery. Our results suggest that these uncommon CTD kinases selectively place phospho-Thr4 marks to regulate expression of targeted genes.

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Section: Resultsmentioning

confidence: 91%

Noncanonical CTD kinases regulate RNA polymerase II in a gene-class-specific manner

et al. 2018

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“… 82 Furthermore, it has also been reported that the gsk3 gene is involved in the regulation of ribosome biogenesis in yeast cells. 83 …”

Section: Discussionmentioning

confidence: 99%

The potential of the newly isolated thermotolerant yeast Pichia kudriavzevii RZ8-1 for high-temperature ethanol production

Chamnipa

Thanonkeo

Klanrit

et al. 2018

Brazilian Journal of Microbiology

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High potential, thermotolerant, ethanol-producing yeasts were successfully isolated in this study. Based on molecular identification and phylogenetic analysis, the isolated thermotolerant yeasts were clustered in the genera of Pichia kudriavzevii, Candida tropicalis, Candida orthopsilosis, Candida glabrata and Kodamea ohmeri. A comparative study of ethanol production using 160 g/L glucose as a substrate revealed several yeast strains that could produce high ethanol concentrations at high temperatures. When sugarcane bagasse (SCB) hydrolysate containing 85 g/L glucose was used as a substrate, the yeast strain designated P. kudriavzevii RZ8-1 exhibited the highest ethanol concentrations of 35.51 g/L and 33.84 g/L at 37 °C and 40 °C, respectively. It also exhibited multi-stress tolerance, such as heat, ethanol and acetic acid tolerance. During ethanol fermentation at high temperature (42 °C), genes encoding heat shock proteins (ssq1 and hsp90), alcohol dehydrogenases (adh1, adh2, adh3 and adh4) and glyceraldehyde-3-phosphate dehydrogenase (tdh2) were up-regulated, suggesting that these genes might play a crucial role in the thermotolerance ability of P. kudriavzevii RZ8-1 under heat stress. These findings suggest that the growth and ethanol fermentation activities of this organism under heat stress were restricted to the expression of genes involved not only in heat shock response but also in the ethanol production pathway.

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“…In this case, the silencing domain in Rap1 may be involved, as are the 60S ribosomal subunit assembly (Mizuta et al 1998;Li et al 2000;Miyoshi et al 2002;Zhao et al 2003;Horigome et al 2008) and relocation of nuclear proteins to the cytoplasm (Nanduri and Tartakoff 2001). Cell wall integrity and stress response component (Wsc) proteins, Pkc1, glycogen synthase kinase-3, the Arp2/3 complex, and spindle pole body components such as Mps3 have also been implicated (Nierras and Warner 1999;Li et al 2000;Yabuki et al 2014Yabuki et al , 2017. In contrast, the Ire1-mediated unfolded protein response, which is typically coordinated with the heat-shock response (Liu and Chang 2008;Hou et al 2014), is not required (Nierras and Warner 1999), although it is otherwise essential for the survival of secretory mutants (Chang et al 2004).…”

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confidence: 99%

Sphingolipid/Pkh1/2-TORC1/Sch9 Signaling Regulates Ribosome Biogenesis in Tunicamycin-Induced Stress Response in Yeast

Yabuki¹,

Ikeda²,

Araki³

et al. 2019

Genetics

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Reduced ribosome biogenesis in response to environmental conditions is a key feature of cell adaptation to stress. For example, ribosomal genes are transcriptionally repressed when cells are exposed to tunicamycin, a protein glycosylation inhibitor that induces endoplasmic reticulum stress and blocks vesicular trafficking in the secretory pathway. Here, we describe a novel regulatory model, in which tunicamycin-mediated stress induces the accumulation of long-chain sphingoid bases and subsequent activation of Pkh1/2 signaling, which leads to decreased expression of ribosomal protein genes via the downstream effectors Pkc1 and Sch9. Target of rapamycin complex 1 (TORC1), an upstream activator of Sch9, is also required. This pathway links ribosome biogenesis to alterations in membrane lipid composition under tunicamycin-induced stress conditions. Our results suggest that sphingolipid/Pkh1/2-TORC1/Sch9 signaling is an important determinant for adaptation to tunicamycin-induced stress.

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Glycogen synthase kinase-3 is involved in regulation of ribosome biogenesis in yeast

Cited by 8 publications

References 25 publications

Noncanonical CTD kinases regulate RNA polymerase II in a gene-class-specific manner

Noncanonical CTD kinases regulate RNA polymerase II in a gene-class-specific manner

The potential of the newly isolated thermotolerant yeast Pichia kudriavzevii RZ8-1 for high-temperature ethanol production

Sphingolipid/Pkh1/2-TORC1/Sch9 Signaling Regulates Ribosome Biogenesis in Tunicamycin-Induced Stress Response in Yeast

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