Glycogen synthase kinase‐3 regulation of chromatin segregation and cytokinesis in mouse preimplantation embryos

Acevedo, Nicole; Wang, Xia; Dunn, Rodney L.; Smith, Gary D.

doi:10.1002/mrd.20495

Cited by 31 publications

(27 citation statements)

References 71 publications

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“…Ser 9 -phosphorylated inactive GSK3B was detected in mice MII oocytes (Acevedo et al 2007b), and we found that this form was more abundant in mature bovine oocytes compared with immature ones. Similarly, in Xenopus, GSK3B was active in GV oocytes and inactivated during maturation in response to progesterone (Fisher et al 1999).…”

Section: Gsk3b Expression In Bovine Oocytes and Surrounding Folliculamentioning

confidence: 67%

“…Bars: 50 mm. (Acevedo et al 2007b), was used in these studies. The addition of 20 mM of LiCl 2 to bovine COC during IVM led to reduced expansion of cumulus after 24 h of IVM as compared with control ( Fig.…”

Section: Effect Of Gsk3 Inhibitors On Bovine Oocyte Nuclear Maturatiomentioning

confidence: 99%

“…Effectively, the expression of GSK3A and GSK3B varied in different tissues of mammals with a poor correlation between mRNA and protein levels, reflecting the differential regulation of transcription and translation for these highly similar kinases (Woodgett 1990, Lau et al 1999. GSK3A mRNA was not detected in mice oocytes and embryos, whereas both GSK3A and GSK3B proteins were evidenced and differentially phosphorylated (Wang et al 2003, Acevedo et al 2007b. GSK3 is involved in the regulation of transcription by phosphorylation or destabilisation of transcriptional factors, maintaining them in an inactive state under basal conditions .…”

Section: Gsk3b Expression In Bovine Oocytes and Surrounding Folliculamentioning

confidence: 99%

“…In vertebrates, GSK3 was shown to be involved in regulation of meiosis progression in frog oocytes (Fisher et al 1999, Sarkissian et al 2004) and in both male and female germ cells in mice (Guo et al 2003, Wang et al 2003, Liu et al 2007. In mice, GSK3 is also involved in the regulation of chromatin segregation and cytokinesis in early embryo (Acevedo et al 2007b). However, few data are currently available on GSK3 expression in ovary of human or large domestic animals and its involvement in oocyte maturation control.…”

Section: Introductionmentioning

confidence: 99%

“…The mechanism of lithium double action on reducing GSK3 activity is well studied (Jope 2003) and LiCl 2 inhibits GSK3, but not MPF activity (Meijer et al 2004). GSK3-specific inhibitors as alsterpaullone and kenpaullone act in a similar way as LiCl 2 in mouse embryonic cells (Acevedo et al 2007b, Kaladchibachi et al 2007). We first analysed GSK3 expression and phosphorylation patterns in oocytes and surrounding granulosa during folliculogenesis and throughout oocyte maturation by immunocytochemistry.…”

Section: Introductionmentioning

confidence: 99%

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Glycogen synthase kinase 3B in bovine oocytes and granulosa cells: possible involvement in meiosis during in vitro maturation

Uzbekova¹,

Salhab²,

Perreau³

et al. 2009

Reproduction

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Glycogen synthase kinase 3 (GSK3) regulates cellular metabolism and cell cycle via different signalling pathways. In response to insulin and growth factors GSK3 is serine-phosphorylated and inactivated. We analysed GSK3B expression and activation in bovine cumulus cells (CC) and oocytes at different meiotic stages in vitro in parallel with MAP kinases ERK (MAPK3/MAPK1) and p38 (MAPK14). GSK3B localised to cytoplasm in granulosa cells and in oocytes throughout folliculogenesis. In mature metaphase-II (MII) oocytes, GSK3B was concentrated to the region of midzone between the oocyte and the first polar body, as well as active phospho-Thr Aurora A kinase (AURKA). During in vitro maturation (IVM), in oocytes, phospho-Ser 9 -GSK3B level increased as well as phospho-MAPK3/MAPK1, while phospho-MAPK14 decreased. In CC, phospho-MAPK14 increased upon germinal vesicle breakdown (GVBD)/metaphase-I (MI) and then decreased during transition to MII. Administration of inhibitors of GSK3 activity (lithium chloride or 2 0 Z,3 0 E -6-bromoindirubin-3 0 -oxime) rapidly increased phospho-Ser 9 -GSK3B, and led to transient decrease of phospho-MAPK3/MAPK1 and to durable enhancing of phospho-MAPK14 in granulosa primary cell culture. GSK3 inhibitors during IVM diminished cumulus expansion and delayed meiotic progression. In cumulus, phospho-MAPK14 level was significantly higher in the presence of inhibitors, comparing with control, through the time of MI/MII transition. In oocytes, phospho-GSK3B was increased and phospho-MAPK3/MAPK1 was decreased before GVBD and oocytes were mainly arrested at MI. Therefore, GSK3B might regulate oocyte meiosis, notably MI/MII transition being the part of MAPK3/1 and MAPK14 pathways in oocytes and CC. GSK3B might be also involved in the local activation of AURKA that controls this transition.

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Section: Gsk3b Expression In Bovine Oocytes and Surrounding Folliculamentioning

confidence: 67%

Section: Effect Of Gsk3 Inhibitors On Bovine Oocyte Nuclear Maturatiomentioning

confidence: 99%

Section: Gsk3b Expression In Bovine Oocytes and Surrounding Folliculamentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Glycogen synthase kinase 3B in bovine oocytes and granulosa cells: possible involvement in meiosis during in vitro maturation

Uzbekova¹,

Salhab²,

Perreau³

et al. 2009

Reproduction

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Tuning of β-catenin activity is required to stabilize self-renewal of rat embryonic stem cells

et al. 2013

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Stabilization of b-catenin, through inhibition of glycogen synthase kinase 3 (GSK3) activity, in conjunction with inhibition of mitogen-activated protein kinase kinase 1/2 (MEK) promotes self-renewal of na€ ıve-type mouse embryonic stem cells (ESC). In developmentally more advanced, primed-type, epiblast stem cells, however, b-catenin activity induces differentiation. We investigated the response of rat ESCs to b-catenin signaling and found that when maintained on feeder-support cells in the presence of a MEK inhibitor alone (1i culture), the derivation efficiency, growth, karyotypic stability, transcriptional profile, and differentiation potential of rat ESC cultures was similar to that of cell lines established using both MEK and GSK3 inhibitors (2i culture). Equivalent mouse ESCs, by comparison, differentiated in identical 1i conditions, consistent with insufficient b-catenin activity. This interspecies difference in reliance on GSK3 inhibition corresponded with higher overall levels of b-catenin activity in rat ESCs. Indeed, rat ESCs displayed widespread expression of the mesendoderm-associated b-catenin targets, Brachyury and Cdx2 in 2i medium, and overt differentiation upon further increases in b-catenin activity. In contrast, mouse ESCs were resistant to differentiation at similarly elevated doses of GSK3 inhibitor. Interestingly, without feeder support, moderate levels of GSK3 inhibition were necessary to support effective growth of rat ESC, confirming the conserved role for b-catenin in ESC self-renewal. This work identifies b-catenin signaling as a molecular rheostat in rat ESC, regulating self-renewal in a dose-dependent manner, and highlights the potential importance of controlling flux in this signaling pathway to achieve effective stabilization of na€ ıve pluripotency.

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A New Chemical Approach to the Efficient Generation of Mouse Embryonic Stem Cells

Baharvand

Hassani

2013

Methods in Molecular Biology

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Here, we present a highly efficient and reproducible method for the establishment of mouse embryonic stem cells (mESCs) from embryonic day 3.5 (E3.5) whole blastocysts. This protocol involves the use of small molecules SB431542 and PD0325901, which inhibit transforming growth factor-β (TGF-β) and extracellular signal-regulated kinases (ERK1/2), respectively. This protocol is universal in the derivation of mESC lines from NMRI, C57BL/6, BALB/c, DBA/2, and FVB/N strains, which have previously been considered refractory or non-permissive for ESC establishment. The efficiency of mESC lines generation is 100%, regardless of genetic background.

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Glycogen synthase kinase‐3 regulation of chromatin segregation and cytokinesis in mouse preimplantation embryos

Cited by 31 publications

References 71 publications

Glycogen synthase kinase 3B in bovine oocytes and granulosa cells: possible involvement in meiosis during in vitro maturation

Glycogen synthase kinase 3B in bovine oocytes and granulosa cells: possible involvement in meiosis during in vitro maturation

Tuning of β-catenin activity is required to stabilize self-renewal of rat embryonic stem cells

A New Chemical Approach to the Efficient Generation of Mouse Embryonic Stem Cells

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