Glycolipid composition of blood group P erythrocytes

Glycolipid pattern of stomach tissue of a human with the rare blood group A,p

“…These are present, however as major components in stomach tissue of the B individual (lane 6). The erythrocyte glycolipids of an A,p individual (lane 3) also lack Pk and P glycolipids in agreement with [7,8]. The faint bands shown for this sample in the 3-sugar region (travelling somewhat faster than the double band of Pk glycolipid) are not due to Pk glycolipid but to lactotriaosylceramide (table 1).…”

“…In contrast to the P1 antigen which is a minor component as most antigens are, Pk and P antigens are directly chemically detectable in lipid extracts. The immunochemical results concerning Pk and P antigens [3,4] could be confirmed therefore by two independent studies of glycolipids prepared from p and Pk erythrocytes, respectively [7,8]. As expected, the p erythrocytes apparently lacked the Pk and P glycolipids [7 $1.…”

“…Also, diglycosylceramide with longer-chain non-hydroxy fatty acids (upper of two bands) is relatively more abundant in p erythrocyte, a fact discussed in [7][8][9]191. We have shown by mass spectrometry that the fatty acids of this upper band of diglycosylceramide [7,9,19] and those of the fucolipids with 6,8 and 9 sugars (only one band each) are identical [20], that is, mainly 22:0,24:0 and 24: 1 acids.…”

“…In fact, p fibroblasts are unable to synthetize Pk and P glycolipids [lo], and P glycolipid may be absent in kidney of p individuals (Kos'cielak, personal communication). On the other hand, the glycolipid situation of p individuals has been chemically documented only for erythrocytes so far [7,8 ] …”

Isolation and Characterization of Poly(glycosyl)ceramides (Megaloglycolipids) with A, H and I Blood‐Group Activities

“…For the determination of the carbohydrate composition, the substances under study were acetolysed and the liberated sugars converted to alditol acetates according to Yang and Hakomori [19]. Hexosaminitol and alditol acetates were then separated and quantitated on 200 x 0.4-cm glass columns packed with 1 o/, OV 225 as previously described [20]. Alditol acetates derived from neutral sugars were additionally analysed on 210 x 0.4-cm columns packed with 3 % ECNSS-M on Gas Chrom Q at 190 "C. The latter column allowed better resolution of galactitol and glucitol acetates as well as the separation of fucitol acetate from a small unidentified peak.…”