Glycomic Characterization of Respiratory Tract Tissues of Ferrets

Jia, Nan; Barclay, William; Roberts, Kim L.; Yen, Hui-Ling; Chan, Wing-Yee; Lam, Alfred; Air, Gillian M.; Peiris, Malik; Dell, Anne; Nicholls, John M.; Haslam, Stuart M.

doi:10.1074/jbc.m114.588541

Cited by 89 publications

(65 citation statements)

References 43 publications

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“…Although glycomics profiling of an airway epithelial cell line (Chandrasekaran et al, 2008), human and ferret respiratory lung tissues (Jia et al, 2014; Walther et al, 2013), and swine respiratory epithelium (Bateman et al, 2010), suggest the presence of extended branched N-glycans similar to those studied here, definitive analysis of human airway epithelium and, in particular, different sections of the airway, still remain to be carried out. A recent report suggesting that the soft palate of ferrets plays a prominent role in selection of HA mutations that promote a switch from avian-type to human-type receptor specificity (Lakdawala et al, 2015), emphasizes how potential variation in the expression of receptor glycans in different regions of the upper airway could account for selection of viruses with specificity for extended, human-type receptors.…”

Section: Discussionmentioning

confidence: 76%

“…Thus, these terms belie the true complexity of the glycome and the potential for other aspects of glycan structure to be an important factor in receptor recognition. Glycan profiling of a human airway epithelial cell line (Chandrasekaran et al, 2008), human and ferret respiratory tract tissues (Jia et al, 2014; Walther et al, 2013), and porcine airway epithelial cells (Bateman et al, 2010) has revealed the presence of Asn-linked glycans (N-glycans) with extended branches as a unique characteristic of the airway glycome. While the branches of N-glycans on most cell types extend from the mannose core (Man 3 GlcNAc 2 Asn) by a single LacNAc (Galβ1-4GlcNAc) sequence, N-glycans in airway tissues exhibit extensions with multiple LacNAc repeats.…”

Section: Introductionmentioning

confidence: 99%

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Recent H3N2 Viruses Have Evolved Specificity for Extended, Branched Human-type Receptors, Conferring Potential for Increased Avidity

Peng

Vries

Grant

et al. 2017

Cell Host & Microbe

185

245

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SUMMARY Human and avian influenza viruses recognize different sialic acid-containing receptors, referred to as human-type (NeuAcα2-6Gal) and avian-type (NeuAcα2-3Gal) respectively. This presents a species barrier for aerosol droplet transmission of avian viruses in humans and ferrets. Recent reports have suggested that current human H3N2 viruses no longer have strict specificity towards human-type receptors. Using an influenza receptor glycan microarray with extended airway glycans we find that H3N2 viruses have in fact maintained human-type specificity, but have evolved preference for a subset of receptors comprising branched glycans with extended poly-N-acetyl-lactosamine (poly-LacNAc) chains, a specificity shared with the 2009 pandemic H1N1 (Cal/04) hemagglutinin. Lipid-linked versions of extended sialoside receptors can restore susceptibility of sialidase-treated MDCK cells to infection by both recent (A/Victoria/361/11) and historical (A/Hong Kong/8/1968) H3N2 viruses. Remarkably, these human-type receptors with elongated branches have the potential to increase avidity by simultaneously binding to two subunits of a single hemagglutinin trimer.

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Section: Discussionmentioning

confidence: 76%

Section: Introductionmentioning

confidence: 99%

Recent H3N2 Viruses Have Evolved Specificity for Extended, Branched Human-type Receptors, Conferring Potential for Increased Avidity

Peng

Vries

Grant

et al. 2017

Cell Host & Microbe

185

245

View full text Add to dashboard Cite

show abstract

“…This is significant as ferrets are widely used to study influenza virus infection and transmission as the expression and distribution of sialic acids in the ferret respiratory tract is similar to humans, although not identical (Jia et al, 2014; van Riel et al, 2006). Neu5Gc may act as a decoy receptor for influenza viruses rather than a functional receptor, as shown using a Neu5Gc-expressing human lung cell line.…”

Section: Influenza Virusesmentioning

confidence: 99%

The Interaction between Respiratory Pathogens and Mucus

Zanin

Baviskar

Webster

et al. 2016

Cell Host & Microbe

245

228

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Summary The interaction between respiratory pathogens and their hosts is complex and incompletely understood. This is particularly true when pathogens encounter the mucus layer covering the respiratory tract. The mucus layer provides an essential first host barrier to inhaled pathogens that can prevent pathogen invasion and subsequent infection. Respiratory mucus has numerous functions and interactions, both with the host and with pathogens. This review summarizes the current understanding of respiratory mucus and its interactions with the respiratory pathogens Pseudomonas aeruginosa, respiratory syncytial virus and influenza viruses, with particular focus on influenza virus transmissibility and host-range specificity. Based on current findings we propose that respiratory mucus represents an understudied host-restriction factor for influenza virus.

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“…However, several differences between the ferret and human respiratory tract have been identified which could influence the outcome of infection. For example, the presence of Sda epitopes that carry ␣-2,3-NeuAc in the ferret respiratory tract is hypothesized to reduce potential binding sites for avian influenza viruses (43,44). The HA of H5Nx viruses possess the key residues Gln226 and Gly228 (H3 numbering) required for 2,3-linked sialic acid binding, indicating the virus has not adapted toward human-type receptor specificity.…”

Section: Figmentioning

confidence: 99%

Pathogenesis and Transmission of Novel Highly Pathogenic Avian Influenza H5N2 and H5N8 Viruses in Ferrets and Mice

Pulit-Penaloza

Sun

Creager

et al. 2015

J Virol

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A novel highly pathogenic avian influenza (HPAI) H5N8 virus, first detected in January 2014 in poultry and wild birds in South Korea, has spread throughout Asia and Europe and caused outbreaks in Canada and the United States by the end of the year. The spread of H5N8 and the novel reassortant viruses, H5N2 and H5N1 (H5Nx), in domestic poultry across multiple states in the United States pose a potential public health risk. To evaluate the potential of cross-species infection, we determined the pathogenicity and transmissibility of two Asian-origin H5Nx viruses in mammalian animal models. The newly isolated H5N2 and H5N8 viruses were able to cause severe disease in mice only at high doses. Both viruses replicated efficiently in the upper and lower respiratory tracts of ferrets; however, the clinical symptoms were generally mild, and there was no evidence of systemic dissemination of virus to multiple organs. Moreover, these influenza H5Nx viruses lacked the ability to transmit between ferrets in a direct contact setting. We further assessed viral replication kinetics of the novel H5Nx viruses in a human bronchial epithelium cell line, Calu-3. Both H5Nx viruses replicated to a level comparable to a human seasonal H1N1 virus, but significantly lower than a virulent Asian-lineage H5N1 HPAI virus. Although the recently isolated H5N2 and H5N8 viruses displayed moderate pathogenicity in mammalian models, their ability to rapidly spread among avian species, reassort, and generate novel strains underscores the need for continued risk assessment in mammals. O n 16 December 2014, the U.S. Department of Agriculture (USDA) confirmed the presence of a novel highly pathogenic avian influenza (HPAI) H5N8 virus in a captive gyrfalcon and H5N2 virus in a northern pintail duck from Whatcom County, Washington (1). This represented the first appearance of HPAI H5 virus in the United States since 2004 when H5N2 subtype virus was confirmed on a poultry farm in Texas (2). HPAI H5N8 viruses were first reported in duck farms in eastern China in 2010, and in early 2014 a novel reassortant H5N8 virus was detected in poultry in South Korea (3-5). The novel virus, belonging to Eurasian lineage clade 2.3.4.4 (formerly clade 2.3.4.6), subsequently spread to China, Japan, and five countries in Europe (6, 7). In November 2014, the virus was detected on chicken and turkey farms in British Columbia, Canada, followed by Washington State, USA, the following month (8). The novel H5N8 viruses continue to spread to multiple regions and have been found in three North America flyways (Pacific, Central, and Mississippi), where wild-bird migrations occur (USDA, Animal and Plant Health Inspection Service [http://www.aphis.usda.gov]). In addition, genetic reassortment with circulating North American avian influenza viruses has resulted in novel HPAI viruses, including H5N2 and H5N1 subtypes. These novel reassortant viruses carry a Eurasian-origin hemagglutinin (HA) gene genetically related to H5N8 viruses detected in South Korea in 2014 and the neura...

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Glycomic Characterization of Respiratory Tract Tissues of Ferrets

Cited by 89 publications

References 43 publications

Recent H3N2 Viruses Have Evolved Specificity for Extended, Branched Human-type Receptors, Conferring Potential for Increased Avidity

Recent H3N2 Viruses Have Evolved Specificity for Extended, Branched Human-type Receptors, Conferring Potential for Increased Avidity

The Interaction between Respiratory Pathogens and Mucus

Pathogenesis and Transmission of Novel Highly Pathogenic Avian Influenza H5N2 and H5N8 Viruses in Ferrets and Mice

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