Glycosaminoglycan concentration in synovium and other tissues of rabbit knee in relation to synovial hydraulic resistance.

Price, F M; Levick, J. R.; Mason, Roger M.

doi:10.1113/jphysiol.1996.sp021634

Cited by 58 publications

(104 citation statements)

References 36 publications

Supporting

Mentioning

101

Contrasting

Order By: Relevance

“…Specifically, the SF PRG4 concentration decreases following acute injury, as does the frictionlowering property of such SF (Elsaid et al, 2005), while PRG4 concentration increases in patients undergoing arthrocentesis procedures (Schmid et al, 2001a). HA (Belcher et al, 1997) and SAPL (Rabinowitz et al, 1984) also exhibit decreased concentrations in osteoarthritis, and HA is also decreased with failure of artificial joints (Mazzucco et al, 2004). The mechanisms by which altered SF lubricant composition occur are unknown.…”

Section: Me Blewis 1 Ge Nugent-derfus 1 Model Of Synovial Fluid Lubmentioning

confidence: 99%

“…The total surface area in the human knee joint is 121 cm 2 for cartilage (Eckstein et al, 2001) and 277 cm 2 for synovium (Davies, 1946), and the synovial fluid volume enclosed by these surfaces is 1.1 ml (Ropes et al, 1940). The synovium, which is 50 µm thick in humans (Price et al, 1995), has a cell volume fraction varying from 0.42 at the surface to 0.67 in deeper layers and a tortuosity due to the cells of 0.69 (Levick and McDonald, 1989a;Levick and McDonald, 1989b). The volume fraction due to collagen is 0.2 (Levick, 1994) and the tortuosity is 0.70 (Maroudas, 1970;Sullivan and Hertel, 1942).…”

Section: Model Parametersmentioning

confidence: 99%

“…1), presents complexities that make it difficult to measure continuously the in vivo SF lubricant composition, and also the main determinants of dynamics in this composition. General models of fluid flow and solute transport through a matrix (reviewed in (Curry, 1984;Levick, 1987)) have been developed, and extensive analysis of the synovium structure has been performed to obtain values for functional parameters that control its transport properties (Levick, 1994;Levick and McDonald, 1989a;Levick and McDonald, 1989b;Price et al, 1996;Price et al, 1995;Sabaratnam et al, 2005;Scott et al, 2003). Knowledge of the structure of synovium, along with related published data, has been applied to the models of fluid and solute transport to specifically model the flow of albumin from synovial capillaries into SF and out through the synovial membrane (Levick, 1994).…”

Section: Me Blewis 1 Ge Nugent-derfus 1 Model Of Synovial Fluid Lubmentioning

confidence: 99%

“…(Haubeck et al, 1995). Cellular secretion rates for synoviocytes were converted to tissue secretion rates using a ~0.8 surface area fraction of synovium occupied by synoviocytes (Price et al, 1995) and assuming a typical cell diameter of ~16 µm.…”

Section: Model Parametersmentioning

confidence: 99%

“…It is backed by a thicker layer (~100 µm) of loose connective tissue called the subsynovium (SUB) that includes an extensive system of lymphatics for clearance of transported molecules. The cells in the synovium form a discontinuous layer separated by intercellular gaps of several microns in width (Knight and Levick, 1984;McDonald and Levick, 1988). The extracellular matrix in these gaps contains collagen types I, III, and V (Ashhurst et al, 1991;Rittig et al, 1992), hyaluronan (Worrall et al, 1991), chondroitin sulphate (Price et al, 1996;Worrall et al, 1994), biglycan and decorin proteoglycans (Coleman et al, 1998a), and fibronectin (Poli et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

A model of synovial fluid lubricant composition in normal and injured joints

Blewis

Nugent-Derfus²,

Schmidt³

et al. 2007

eCM

126

View full text Add to dashboard Cite

The synovial fluid (SF) of joints normally functions as a biological lubricant, providing low-friction and low-wear properties to articulating cartilage surfaces through the putative contributions of proteoglycan 4 (PRG4), hyaluronic acid (HA), and surface active phospholipids (SAPL). These lubricants are secreted by chondrocytes in articular cartilage and synoviocytes in synovium, and concentrated in the synovial space by the semi-permeable synovial lining. A deficiency in this lubricating system may contribute to the erosion of articulating cartilage surfaces in conditions of arthritis. A quantitative intercompartmental model was developed to predict in vivo SF lubricant concentration in the human knee joint. The model consists of a SF compartment that (a) is lined by cells of appropriate types, (b) is bound by a semi-permeable membrane, and (c) contains factors that regulate lubricant secretion. Lubricant concentration was predicted with different chemical regulators of chondrocyte and synoviocyte secretion, and also with therapeutic interventions of joint lavage and HA injection. The model predicted steady-state lubricant concentrations that were within physiologically observed ranges, and which were markedly altered with chemical regulation. The model also predicted that when starting from a zero lubricant concentration after joint lavage, PRG4 reaches steady-state concentration ~10-40 times faster than HA. Additionally, analysis of the clearance rate of HA after therapeutic injection into SF predicted that the majority of HA leaves the joint after ~1-2 days. This quantitative intercompartmental model allows integration of biophysical processes to identify both environmental factors and clinical therapies that affect SF lubricant composition in whole joints.

show abstract

Section: Me Blewis 1 Ge Nugent-derfus 1 Model Of Synovial Fluid Lubmentioning

confidence: 99%

Section: Model Parametersmentioning

confidence: 99%

Section: Me Blewis 1 Ge Nugent-derfus 1 Model Of Synovial Fluid Lubmentioning

confidence: 99%

Section: Model Parametersmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

A model of synovial fluid lubricant composition in normal and injured joints

Blewis

Nugent-Derfus²,

Schmidt³

et al. 2007

eCM

126

View full text Add to dashboard Cite

show abstract

Interaction of intraarticular hyaluronan and albumin in the attenuation of fluid drainage from joints

Scott

Coleman

Mason

et al. 2000

Arthritis & Rheumatism

View full text Add to dashboard Cite

Hyaluronan and albumin act together at normal concentrations to conserve synovial fluid in the presence of raised drainage pressures. Hyaluronan has the greater effect, acting osmotically by way of a concentration polarization boundary layer. Attenuation of this effect in arthritic effusions with low hyaluronan concentrations is one of several factors limiting fluid accumulation and, hence, the size of the effusion.

show abstract

Treatment of murine collagen-induced arthritis by ex vivo extracellular superoxide dismutase gene transfer

Iyama¹,

Okamoto²,

Sato³

et al. 2001

Arthritis & Rheumatism

View full text Add to dashboard Cite

Objective Superoxide dismutase (SOD) is a potent antiinflammatory enzyme that has received growing attention for its therapeutic potential. This study was undertaken to examine the efficacy of extracellular SOD (EC‐SOD) gene therapy in murine collagen‐induced arthritis. Methods Embryonic DBA/1 mouse fibroblasts were infected with a recombinant retrovirus expressing human EC‐SOD. DBA/1 mice that had been treated with type II collagen were administered subcutaneous injections of 2 × 107 EC‐SOD–expressing fibroblasts on day 29, when symptoms of arthritis were already present. The severity of arthritis in individual mice was evaluated in a double‐blind manner; each paw was assigned a separate clinical score, and hind paw thickness was measured with a caliper. Mice were killed on day 50 for histologic examination of the joints. Results High serum concentrations of EC‐SOD were maintained for at least 7 days. Mice treated with the transgene exhibited significant suppression of clinical symptoms such as disabling joint swelling, deformity, and hind paw thickness, compared with the untreated group (mean ± SD maximum clinical score in the untreated and the transgene‐treated groups 2.71 ± 1.08 and 1.35 ± 1.22, respectively; P < 0.01, and hind paw thickness 3.04 ± 0.18 mm and 2.56 ± 0.12 mm, respectively; P < 0.05). Histologic abnormalities, including destruction of cartilage and bone, infiltration of mononuclear cells, and proliferation of synovial cells, were also markedly improved in the EC‐SOD–treated mice compared with the control group (histopathologic score 7.50 ± 1.13 and 4.13 ± 1.88 in the untreated and transgene‐treated groups, respectively; P < 0.05). Conclusion These results indicate that EC‐SOD gene transfer may be an effective form of therapy for rheumatoid arthritis.

show abstract

Glycosaminoglycan concentration in synovium and other tissues of rabbit knee in relation to synovial hydraulic resistance.

Cited by 58 publications

References 36 publications

A model of synovial fluid lubricant composition in normal and injured joints

A model of synovial fluid lubricant composition in normal and injured joints

Interaction of intraarticular hyaluronan and albumin in the attenuation of fluid drainage from joints

Treatment of murine collagen-induced arthritis by ex vivo extracellular superoxide dismutase gene transfer

Contact Info

Product

Resources

About