Glycosphingolipid-blotting: an immunological detection procedure after separation by thin layer chromatography

Towbin, Harry; Schoenenberger, Cora‐Ann; Ball, Roland K.; Braun, Dietmar; Rosenfelder, Günter

doi:10.1016/0022-1759(84)90015-2

Cited by 64 publications

(27 citation statements)

References 10 publications

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“…The separated proteins were transferred on PVDF (polyvinylidene difluoride) membranes (Bio-Rad Laboratories) according to Towbin et al (1984). Equal loading of samples was checked by Ponceau dye staining of the transferred gels.…”

Section: Methodsmentioning

confidence: 99%

In VivoNMDA Receptor Activation Accelerates Motor Unit Maturation, Protects Spinal Motor Neurons, and Enhances SMN2 Gene Expression in Severe Spinal Muscular Atrophy Mice

Biondi

Branchu²,

Sanchez³

et al. 2010

J. Neurosci.

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Section: Methodsmentioning

confidence: 99%

In VivoNMDA Receptor Activation Accelerates Motor Unit Maturation, Protects Spinal Motor Neurons, and Enhances SMN2 Gene Expression in Severe Spinal Muscular Atrophy Mice

Biondi

Branchu²,

Sanchez³

et al. 2010

J. Neurosci.

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“…A similar approach was employed for immunological detection of glycosphingolipids over a decade previously [25]. Based on the studies of Dowler et al [18], Echelon Inc. began in the late 1990s to market PIP Strips™, in which an array of phosphoinositides are pre-spotted (100 pmoles per spot) on nitrocellulose.…”

Section: Fat Blots/dot-blots/lipid Westerns For Analyzing Binding To mentioning

confidence: 99%

Determining selectivity of phosphoinositide-binding domains

Narayan

Lemmon

2006

Methods

119

121

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The burgeoning of phosphoinositide-binding domains and proteins in cellular signaling and trafficking has drawn laboratories from a wide variety of fields into the study of lipid interactions with peripheral membrane proteins. Many different approaches have been developed to assess phosphoinositide binding, some of which are more problematic than others, and some of which can be quantitated more readily than others. With a focus on the methods used in our laboratory, we describe here the considerations that need to be taken into account when establishing -and quantitating -the specific binding of a protein or domain to phosphoinositides in membranes. We also discuss briefly a few examples in which no clear consensus has yet been reached as to the specificity of a given domain or protein because of discrepancies between different commonlyused approaches.

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“…Taking advantage of the very good separation of lipids on an HPTLC plate and the immobilization of lipids on the solid phase, we examined the transfer of glycosphingolipids and phospholipids from an HPTLC plate to a plastic membrane. Towbin et al first reported the transfer of glycosphingolipids using nitrocellulose membranes (7). But transfer efficiency was poor, and reproducible results were not obtained.…”

Section: Sis (Tlc Blotting/ms) a Binding Assay And The Identi-mentioning

confidence: 98%

TLC Blotting: Application to Microscale Analysis of Lipids and as a New Approach to Lipid–Protein Interaction

Taki

Ishikawa

1997

Analytical Biochemistry

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Glycosphingolipid-blotting: an immunological detection procedure after separation by thin layer chromatography

Cited by 64 publications

References 10 publications

In VivoNMDA Receptor Activation Accelerates Motor Unit Maturation, Protects Spinal Motor Neurons, and Enhances SMN2 Gene Expression in Severe Spinal Muscular Atrophy Mice

In VivoNMDA Receptor Activation Accelerates Motor Unit Maturation, Protects Spinal Motor Neurons, and Enhances SMN2 Gene Expression in Severe Spinal Muscular Atrophy Mice

Determining selectivity of phosphoinositide-binding domains

TLC Blotting: Application to Microscale Analysis of Lipids and as a New Approach to Lipid–Protein Interaction

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