Glycosylated gold nanoparticle libraries for label-free multiplexed lectin biosensing

Richards, Sarah‐Jane; Otten, Lucienne; Gibson, Matthew I.

doi:10.1039/c5tb01994j

Cited by 43 publications

(52 citation statements)

References 45 publications

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“…This is in line with the inhibitory data showing that 75% galactose was the most potent in terms of EC 50 and total inhibition achieved. This rational for optimal binding agrees with previous reports that ~30% mannose is optimal for glyconanoparticles agglutinating RCA 120 . These observations could support an additional binding mode for mannose, and that binding is not only due to the galactose ligands.…”

Section: Resultssupporting

confidence: 91%

“…Multivalency is also observed to result in altered binding affinities compared to monovalent systems. Richards et al have reported that mannosylated gold nanoparticles have affinity toward RCA 120 , despite the monosaccharides having little or no affinity . Sequence‐controlled glycooligomers have shown increased affinity due to the nonbinding glycan providing steric shielding rather than more binding and mixed‐carbohydrate particles have been shown to have increased cell uptake into macrophages over the homogeneous equivalents .…”

Section: Introductionmentioning

confidence: 91%

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Comparison of systematically functionalized heterogeneous and homogenous glycopolymers as toxin inhibitors

Martyn

Biggs

Gibson

2018

J. Polym. Sci. Part A: Polym. Chem.

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Multivalent glycosylated polymers and particles display enhanced binding affinity toward lectins compared to individual glycans. The design of glycopolymers with selectivity toward pathogen‐associated lectins (toxins) for sensing or in antiadhesion therapy is complicated due to lectins having promiscuous binding profiles and can be considered to be pattern recognition “readers,” with the capability to bind to several different glycans. Here, heterogeneous glycopolymers bearing variable densities of two different monosaccharides are synthesized by a three‐step postpolymerization modification approach, enabling systematic control over composition. It is found that heterogeneous polymers displayed increased inhibitory activity, compared to homogeneous polymers, against a RCA120 and the cholera toxin. This demonstrates that embracing heterogeneity in glycomaterials could result in improved performance or emergent properties. © 2018 Wiley Periodicals, Inc. J. Polym. Sci., Part A: Polym. Chem. 2019, 57, 40–47

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Section: Resultssupporting

confidence: 91%

Section: Introductionmentioning

confidence: 91%

Comparison of systematically functionalized heterogeneous and homogenous glycopolymers as toxin inhibitors

Martyn

Biggs

Gibson

2018

J. Polym. Sci. Part A: Polym. Chem.

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“…Lectins have been used in targeted capture using novel approaches such as the self‐propelled microtubular nanoengines developed by Campuzano and others (), where microstructures conjugated to lectins autonomously “swim” in the solution to seek out and bind pathogens such as E. coli without the addition of exogenous labels. Although lectins have a low affinity relative to the other biorecognition agents discussed here, specificity can be significantly increased if multiplexing sensors are used to target pathogen‐associated molecular patterns rather than an individual cell surface target based on a single interaction between target and lectin (Richards and others ). Furthermore, lectins can be easily regenerated for repeated measurements and thus can be used as a regenerable biosensor in processing lines, food packaging, and so on.…”

Section: Comparison Of Current and Emerging Techniques For Monitoringmentioning

confidence: 99%

Emerging Biorecognition and Transduction Schemes for Rapid Detection of Pathogenic Bacteria in Food

Vanegas

Gomes

Cavallaro

et al. 2017

Comp Rev Food Sci Food Safe

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The presence of unsafe levels of microorganisms in food constitutes a growing economic and public health problem that necessitates new technology for their rapid detection along the food continuum from production to consumption. While traditional techniques are reliable, there is a need for more sensitive, selective, rapid, and costeffective approaches for food safety evaluation. Methods such as microbiological counts are sufficiently accurate and inexpensive, and are capable of determining presence and viability for most pathogens. However, these techniques are time consuming, involve destructive sampling, and require trained personnel and biosafety-certified facilities for analysis. Molecular techniques such as the polymerase chain reaction have greatly improved analytical capability over the last decade, achieving shorter analysis time with quantitative data and strain specificity, and in some cases the ability to discriminate cell viability. The emerging field of nanosensors/biosensors has demonstrated a variety of devices that hold promise to bridge the gap between traditional plate counting and molecular techniques. Many nanosensors/biosensors are rapid, portable, accurate devices that can be used as an additional screening tool for identifying unsafe levels of microorganisms in food products with no need for pre-enrichment. In this review, we provide a brief overview of available biorecognitiontransduction techniques for detecting bacteria in food. We then discuss the advantages and disadvantages of each technique, and describe some recent biosensor or nanosensor technologies that are under development. We conclude by summarizing the opportunities and challenges in the field of pathogen monitoring in food systems and we focus the discussion on the strengths/weaknesses of the most popular biorecognition agents and transducer nanomaterials for biosensing.

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“…This works like a tongue – we only have five different tastes, but we are trained to use these to identify foods. We do the same, with a few sugars to identify pathogens [5–7]. …”

Section: Q You Recently Presented Work On a New ‘Chemical Tongue’ Appmentioning

confidence: 99%

From Ice to Bugs: Polymers and Sugars to Address Healthcare Challenges

Gibson

2016

Future Sci. OA

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Matthew I Gibson talks to Francesca Lake, Managing Editor: After completing his PhD at the University of Durham (UK), Matthew moved to the Ecole Polytechnique Federale de Lausanne (Switzerland) where he researched nanoparticle delivery technology. In 2009, he then moved to the University of Warwick (UK), to establish his independent research group. He has since been promoted to assistant (2012), associate (2015) and full (2016) professor. His interdisciplinary group undertakes research with the core theme of using biomaterials to improve health. This spans infection to regenerative medicine, and to fundamental polymer chemistry.

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Glycosylated gold nanoparticle libraries for label-free multiplexed lectin biosensing

Abstract: Glycosylated nanoparticle libraries are developed to enable ‘barcode’ sensing of lectins and toxins.

Cited by 43 publications

References 45 publications

Comparison of systematically functionalized heterogeneous and homogenous glycopolymers as toxin inhibitors

Comparison of systematically functionalized heterogeneous and homogenous glycopolymers as toxin inhibitors

Emerging Biorecognition and Transduction Schemes for Rapid Detection of Pathogenic Bacteria in Food

From Ice to Bugs: Polymers and Sugars to Address Healthcare Challenges

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