Glycosylated zein as a novel nanodelivery vehicle for lutein

Epigallocatechin gallate (EGCG) has many excellent qualities such as its antitumor, antiradiation and anti-oxidation properties, but its application is limited because its oral bioavailability is low and stability is poor. In this paper, zein and gum arabic (GA) were used as wall materials to prepare Zein-GA complex nanoparticles for encapsulating and protecting the EGCG. The particle size of Zein-GA-EGCG complex nanoparticles ranged from 128.03–221.23 nm, and the EGCG encapsulation efficiency reached a maximum of 75.23% when the mass ratio of zein to GA was 1:1. The FTIR and XRD results illustrated that the components of the Zein-GA-EGCG complex nanoparticles interacted by electrostatic, hydrogen bonding, and hydrophobic interactions. The EGCG release rate of Zein-GA-EGCG nanoparticles (16.42%) was lower than that of Zein-EGCG (25.52%) during gastric digestion, and a large amount of EGCG was released during intestinal digestion, suggesting that the Zein-GA-EGCG nanoparticles could achieve the sustained release of EGCG during in vitro digestion. Hence, using Zein-GA complexes to encapsulate EGCG effectively increased the encapsulation efficiency of EGCG and realized the purpose of sustained release during simulated gastrointestinal digestion.

Section: Resultsmentioning

confidence: 99%

Encapsulation of EGCG by Zein-Gum Arabic Complex Nanoparticles and In Vitro Simulated Digestion of Complex Nanoparticles

Jin

Liu

Tong

et al. 2022

“…Yu et al developed the composite nanoparticles from gum Arabic and carboxymethylcellulose-modified stauntonia brachyyanthera seed albumin for lutein delivery, and this system can increase the bioaccessibility of lutein to 46.8% [ 51 ]. Zhang et al utilized glycosylated zein as a delivery vehicle for lutein and obtained a bioaccessibility of about 30% [ 52 ]. Compared with these developed carriers, TSCPN-stabilized Pickering emulsions exhibited better lutein delivery capacity during digestion in vitro.…”

Section: Resultsmentioning

confidence: 99%

Pickering Emulsion Stabilized by Tea Seed Cake Protein Nanoparticles as Lutein Carrier

Liang

Zhu

Zhang

et al. 2022

To effectively deliver lutein, hydrothermally prepared tea seed cake protein nanoparticles (TSCPN) were used to fabricate Pickering emulsion, and the bioaccessibility of lutein encapsulated by Pickering emulsion and the conventional emulsion was evaluated in vitro. The results indicated that the average size and absolute value of zeta potential of TSCPN increased along with the increase in the protein concentration, and 2% protein concentration was adopted to prepare TSCPN. With the increase in the concentration of TSCPN, the size of Pickering emulsion decreased from 337.02 μm to 89.36 μm, and when the TSCPN concentration was greater than 0.6%, all emulsions exhibited good stability during the 14 days storage. Combined with the microstructure result, 1.2% TSCPN was used to stabilize Pickering emulsion. With the increase in ionic concentration (0–400 mM), the particle size of the emulsions increased while the absolute value of zeta potential decreased. TSCPN-based Pickering emulsion was superior to the conventional emulsion for both lutein encapsulation (96.6 ± 1.0% vs. 82.1 ± 1.4%) and bioaccessibility (56.0% ± 1.1% vs. 35.2 ± 1.2%). Thus, TSCPN-based Pickering emulsion in this study have the potential as an effective carrier for lutein.

“…Therefore, many scholars pay attention to the modification of zein, to improve its functional properties and expand its application in the food industry. Currently, research on the modification of zein is mainly divided into two categories: physical modification (the combination of zein and polysaccharide [ 75 ], surfactant [ 76 ], protein [ 77 ], and polyphenol [ 78 ], etc.,) and chemical modification (glycosylation [ 79 ], phosphorylation [ 80 ], deamidation[ 38 ], carboxymethylation [ 81 ], etc.,). The physical modification method can select materials with specific properties (pH response, magnetism, temperature sensitivity, etc.,) and zein solution to form a nanocomposite carrier so that the nanocarriers can quickly release active substances under specific pH, external magnetic field, and different temperature responses [ 82 ].…”

Section: Application Of Bioactive Substances Loaded On Plant Protein-...mentioning

confidence: 99%

New Perspective on Natural Plant Protein-Based Nanocarriers for Bioactive Ingredients Delivery

Wen

Zhang

et al. 2022

The health effects of bioactive substances in the human body are affected by several factors, including food processing conditions, storage conditions, light and heat, among others. These factors greatly limit the stability and bioavailability of bioactive substances. These problems can be solved by a novel protein-based nanocarrier technology, which has the excellent potential to enhance solubility, bioavailability, and the controlled release of bioactive substances. In addition, plant protein has the advantages of economy, environmental protection, and high nutrition compared to animal protein. In this review, the preparation, characterization, and application of plant protein-based nanocarriers are summarized. The research deficiency and future prospects of plant protein nanocarriers are emphasized.