GmEXLB1, a Soybean Expansin-Like B Gene, Alters Root Architecture to Improve Phosphorus Acquisition in Arabidopsis

Kong, Youbin; Wang, Bing; Du, Hui; Li, Wenlong; Li, Xihuan; Zhang, Caiying

doi:10.3389/fpls.2019.00808

Cited by 45 publications

(43 citation statements)

References 61 publications

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“…Expansins consist of four subfamilies, including α-expansin (EXPA), expansin-like A (EXLA), β-expansin (EXPB) and expansin-like B (EXLB) [52]. To data, three expansin members have been implicated in regulating root architecture and morphology when plants subjected to P deficiency, such as TaEXPB23 from wheat [53], GmEXPB2 and GmEXLB1 from soybean [34,54]. In this study, the expressions of SgEXPB2 and SgEXLB1, the homologies of GmEXPB2 and GmEXLB1, were increased by Pi starvation in roots ( Fig.…”

Section: Modification Of Stylo Root Morphology In Response To Low Pi mentioning

confidence: 99%

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Metabolic alterations provide insights into Stylosanthes roots responding to phosphorus deficiency

Luo

Liu

Zhang

et al. 2019

Preprint

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Background: Phosphorus (P) deficiency is one of the major constraints limiting plant growth, especially in acid soils. Stylosanthes (stylo) is a pioneer tropical legume with excellent adaptability to low P stress, but its underlying mechanisms remain largely unknown. Results: In this study, the physiological, molecular and metabolic changes in stylo responding to phosphate (Pi) starvation were investigated. Under low P condition, the root growth in stylo was significantly enhanced, which was accompanied with up-regulation of expansin genes participating in cell wall loosening. Metabolic profiling analysis showed that a total of 256 metabolites with differential accumulation were identified in stylo roots responding to P deficiency, which mainly include flavonoids, sugars, nucleotides, amino acids, phenylpropanoids and phenylamides. P deficiency led to significant reduction in the accumulation of phosphorylated metabolites (e.g., P-containing sugars, nucleotides and cholines), suggesting that internal P utilization was enhanced in stylo roots. However, flavonoid metabolites, such as kaempferol, daidzein and their glycoside derivatives, were significantly increased in P-deficient stylo roots. Furthermore, the transcripts of various genes involved in flavonoids synthesis were found to be up-regulated by Pi starvation in stylo roots. In addition, the abundance of phenolic acids and phenylamides was significantly increased in stylo roots during P deficiency. The enhanced accumulation of the metabolites in stylo roots, such as flavonoids, phenolic acids and phenylamides, might facilitate P solubilization and cooperate with beneficial microorganisms in rhizosphere, and thus contributing to P acquisition and utilization in stylo. Conclusions: These results suggest that stylo plants cope with P deficiency by modulating root morphology, scavenging internal Pi from phosphorylated metabolites and enhancing accumulation of flavonoids, phenolic acids and phenylamides. This study provides valuable insights into the complex responses and adaptive mechanisms of stylo to P deficiency.

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Section: Modification Of Stylo Root Morphology In Response To Low Pi mentioning

confidence: 99%

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confidence: 99%

Metabolic alterations provide insights into Stylosanthes roots responding to phosphorus deficiency

Luo

Liu

Zhang

et al. 2019

Preprint

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“…BrEXLB1 overexpression is consistent with the increase in the size of the elongation zone, thus confirming its role in cell elongation and/or root growth. Previously, Kong et al [27] suggested that soybean EXLB1 may be associated with lateral root emergence. Therefore, we also investigated the BrEXLB1 association with lateral root development by expression profiling of known lateral root marker genes such as GATA23 and MAKR4 genes in OX and OX-AS lines ( Figure S4) and also comparing the lateral root phenotypes.…”

Section: Overexpression Of Brexlb1 Is Positively Associated With the mentioning

confidence: 99%

BrEXLB1, a Brassica rapa Expansin-Like B1 Gene Is Associated with Root Development, Drought Stress Response, and Seed Germination

Muthusamy

Kim

Yoon

et al. 2020

Genes

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Expansins are structural proteins prevalent in cell walls, participate in cell growth and stress responses by interacting with internal and external signals perceived by the genetic networks of plants. Herein, we investigated the Brassica rapa expansin-like B1 (BrEXLB1) interaction with phytohormones (IAA, ABA, Ethephon, CK, GA3, SA, and JA), genes (Bra001852, Bra001958, and Bra003006), biotic (Turnip mosaic Virus (TuMV), Pectobacterium carotovorum, clubroot disease), and abiotic stress (salt, oxidative, osmotic, and drought) conditions by either cDNA microarray or qRT-PCR assays. In addition, we also unraveled the potential role of BrEXLB1 in root growth, drought stress response, and seed germination in transgenic Arabidopsis and B. rapa lines. The qRT-PCR results displayed that BrEXLB1 expression was differentially influenced by hormones, and biotic and abiotic stress conditions; upregulated by IAA, ABA, SA, ethylene, drought, salt, osmotic, and oxidative conditions; and downregulated by clubroot disease, P. carotovorum, and TuMV infections. Among the tissues, prominent expression was observed in roots indicating the possible role in root growth. The root phenotyping followed by confocal imaging of root tips in Arabidopsis lines showed that BrEXLB1 overexpression increases the size of the root elongation zone and induce primary root growth. Conversely, it reduced the seed germination rate. Further analyses with transgenic B. rapa lines overexpressing BrEXLB1 sense (OX) and antisense transcripts (OX-AS) confirmed that BrEXLB1 overexpression is positively associated with drought tolerance and photosynthesis during vegetative growth phases of B. rapa plants. Moreover, the altered expression of BrEXLB1 in transgenic lines differentially influenced the expression of predicted BrEXLB1 interacting genes like Bra001852 and Bra003006. Collectively, this study revealed that BrEXLB1 is associated with root development, drought tolerance, photosynthesis, and seed germination.

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“…Expansins consist of four subfamilies, including α-expansin (EXPA), expansin-like A (EXLA), β-expansin (EXPB) and expansin-like B (EXLB) [53]. To data, three expansin members have been implicated in regulating root architecture and morphology when plants subjected to P deficiency, such as TaEXPB23 from wheat [54], GmEXPB2 and GmEXLB1 from soybean [34,55]. In this study, the expressions of SgEXPB2 and SgEXLB1, the homologies of GmEXPB2 and GmEXLB1, were increased by Pi starvation in roots ( Fig.…”

Section: Modification Of Stylo Root Morphology In Response To Low Pi mentioning

confidence: 99%

“…For example, expansins are superfamily proteins that modify cell wall loosening, leading to cell extension [33]. GmEXLB1, one of Pi starvation-inducible expansin genes, identified by transcriptomic analysis in soybean (Glycine max), has been characterized to be involved in P acquisition by regulating root growth [34]. Recently, a pair of rice vacuolar Pi efflux transporters (OsVPE1 and OsVPE2) identified by vacuolar membrane proteomics, have been demonstrated to participate in vacuole Pi remobilization [35].…”

Section: Introductionmentioning

confidence: 99%

Metabolic alterations provide insights into Stylosanthes roots responding to phosphorus deficiency

et al. 2020

View full text Add to dashboard Cite

Background: Phosphorus (P) deficiency is one of the major constraints limiting plant growth, especially in acid soils. Stylosanthes (stylo) is a pioneer tropical legume with excellent adaptability to low P stress, but its underlying mechanisms remain largely unknown. Results: In this study, the physiological, molecular and metabolic changes in stylo responding to phosphate (Pi) starvation were investigated. Under low P condition, the growth of stylo root was enhanced, which was attributed to the up-regulation of expansin genes participating in root growth. Metabolic profiling analysis showed that a total of 256 metabolites with differential accumulations were identified in stylo roots response to P deficiency, which mainly included flavonoids, sugars, nucleotides, amino acids, phenylpropanoids and phenylamides. P deficiency led to significant reduction in the accumulation of phosphorylated metabolites (e.g., P-containing sugars, nucleotides and cholines), suggesting that internal P utilization was enhanced in stylo roots subjected to low P stress. However, flavonoid metabolites, such as kaempferol, daidzein and their glycoside derivatives, were increased in P-deficient stylo roots. Furthermore, the qRT-PCR analysis showed that a set of genes involved in flavonoids synthesis were found to be up-regulated by Pi starvation in stylo roots. In addition, the abundances of phenolic acids and phenylamides were significantly increased in stylo roots during P deficiency. The increased accumulation of the metabolites in stylo roots, such as flavonoids, phenolic acids and phenylamides, might facilitate P solubilization and cooperate with beneficial microorganisms in rhizosphere, and thus contributing to P acquisition and utilization in stylo. Conclusions: These results suggest that stylo plants cope with P deficiency by modulating root morphology, scavenging internal Pi from phosphorylated metabolites and increasing accumulation of flavonoids, phenolic acids and phenylamides. This study provides valuable insights into the complex responses and adaptive mechanisms of stylo roots to P deficiency.

show abstract

GmEXLB1, a Soybean Expansin-Like B Gene, Alters Root Architecture to Improve Phosphorus Acquisition in Arabidopsis

Cited by 45 publications

References 61 publications

Metabolic alterations provide insights into Stylosanthes roots responding to phosphorus deficiency

Metabolic alterations provide insights into Stylosanthes roots responding to phosphorus deficiency

BrEXLB1, a Brassica rapa Expansin-Like B1 Gene Is Associated with Root Development, Drought Stress Response, and Seed Germination

Metabolic alterations provide insights into Stylosanthes roots responding to phosphorus deficiency

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