GmMPK6 Positively Regulates Salt Tolerance through Induction of GmRbohI1 in Soybean

Abstract

Salt stress is a critical environmental stress that impairs plant growth and development, especially in crop productivity; therefore, understanding the salt response in plants is the basis for their development of salt tolerance. Under salinity, soybean mitogen-activated protein kinase 6 (GmMPK6) is activated and positively regulates reactive oxygen species (ROS) generation. However, it is not yet elucidated how GmMPK6 regulates ROS generation and its role in salt tolerance. Here, we show that GmMPK6, solely activated in NaCl treatment, and gene expression of GmRbohI1 was not only reduced by MPK inhibitor SB202190 in NaCl treatment, but also increased in a GMKK1-expressing protoplast. Furthermore, SB202190 and the NADPH-oxidase inhibitor, diphenyleneiodonium chloride, increased susceptibility to salt stress. The expression of GmRD19A was induced by NaCl treatment, but this expression was compromised by SB202190. Consequently, we revealed that GmMPK6 induces ROS generation through the transcriptional regulation of GmRbohI1 and increases salt tolerance in soybean.

Comprehensive analysis of MAPK gene family in upland cotton (Gossypium hirsutum) and functional characterization of GhMPK31 in regulating defense response to insect infestation

“…NTF6 regulates NADPH Oxidase-Dependent oxidative bursts in N. benthamiana (Asai et al 2008 ), MPK6 mediates salt-induced iron superoxide dismutase gene expression and further induces ROS production (Xing et al 2 015 ). In soybean, GmMPK6 induces ROS generation through the transcriptional regulation of GmRbohI1 and increases salt tolerance in soybean (Son et al 2023 ). Similarly, in this study, we detected an excessive amount of H 2 O 2 in the leaves of OM31 plants and found direct evidence of interaction between GhMPK31 and GhRBOHB.…”

PacBio Full-Length Transcriptome Sequencing Reveals the Mechanism of Salt Stress Response in Sonneratia apetala

“…Meanwhile, MAPK can also induct ROS under salt stress. Son et al [45] found that GmMPK6 induced ROS production through transcriptional regulation of GmRbohI1 and increased salt tolerance in soybean. In our study, 5 MAPK4 indicates a possible important role of the MAPK pathway during the salt stress response of S. apetala (Figure 6a; Supplementary Data S2).…”