GmTMT2a from soybean elevates the α-tocopherol content in corn and Arabidopsis

Zhang, Lan; Luo, Yanzhong; Zhu, Yongxing; Zhang, Liang; Zhang, Wei; Chen, Rumei; Xu, Miaoyun; Fan, Yanting; Wang, Lei

doi:10.1007/s11248-013-9713-8

Cited by 22 publications

(18 citation statements)

References 27 publications

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“…Previous studies have confirmed the function of γ-TMT through mutant or transgenic studies (Bergmuller et al 2003;Lu et al 2013b;Zhang et al 2013), and natural variations in γ-TMT are known to be responsible for the αT content in soybean and maize (Dwiyanti et al 2011;Lipka et al 2013). In our study, nucleotide variations in the coding and promoter region of OsγTMT were associated with variations in the αT content among rice varieties.…”

Section: Discussionsupporting

confidence: 78%

Natural variations in OsγTMT contribute to diversity of the α-tocopherol content in rice

Wang

Yoon

et al. 2015

Mol Genet Genomics

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Tocopherols and tocotrienols, collectively known as tocochromanols, are lipid-soluble molecules that belong to the group of vitamin E compounds. Among them, α-tocopherol (αΤ) is one of the antioxidants with diverse functions and benefits for humans and animals. Thus, understanding the genetic basis of these traits would be valuable to improve nutritional quality by breeding in rice. Genome-wide association study (GWAS) has emerged as a powerful strategy for identifying genes or quantitative trait loci (QTL) underlying complex traits in plants. To discover the genes or QTLs underlying the naturally occurring variations of αΤ content in rice, we performed GWAS using 1.44 million high-quality single-nucleotide polymorphisms acquired from re-sequencing of 137 accessions from a diverse rice core collection. Thirteen candidate genes were found across 2-year phenotypic data, among which gamma-tocopherol methyltransferase (OsγTMT) was identified as the major factor responsible for the αΤ content among rice accessions. Nucleotide variations in the coding region of OsγTMT were significantly associated with the αΤ content variations, while nucleotide polymorphisms in the promoter region of OsγTMT also could partly demonstrate the correlation with αΤ content variations, according to our RNA expression analyses. This study provides useful information for genetic factors underlying αΤ content variations in rice, which will significantly contribute the research on αΤ biosynthesis mechanisms and αΤ improvement of rice.

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Section: Discussionsupporting

confidence: 78%

Natural variations in OsγTMT contribute to diversity of the α-tocopherol content in rice

Wang

Yoon

et al. 2015

Mol Genet Genomics

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“…γ-TMT3 is located on chromosome 9, whereas γ-TMT1 (Glyma.12G014200) and γ-TMT2 (Glyma.12G014300) are located in tandem on chromosome 12 (Dwiyanti et al 2011). Overexpression of γ-TMT2 in corn (maize) and Arabidopsis increased their α-tocopherol contents, indicating that γ-TMT2 is functional (Zhang et al 2013). …”

Section: Introductionmentioning

confidence: 99%

Natural diversity of seed α-tocopherol ratio in wild soybean (<i>Glycine soja</i>) germplasm collection

et al. 2016

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Alpha-tocopherol is one of four tocopherol isoforms and has the highest vitamin E activity in humans. Most cultivated soybean seeds contain γ-tocopherol as the predominant form, and the ratio of α-tocopherol content to total tocopherol content (α-tocopherol ratio) is <10%. Three soybean accessions from Eastern Europe have α-tocopherol ratios of >20%. This higher content is likely due to mutations in the promoter region of the γ-tocopherol methytransferase-3 (γ-TMT3) gene. We surveyed a wild soybean germplasm collection and detected 16 accessions with stable seed α-tocopherol ratios of >20% under different growth conditions. The α-tocopherol ratios were greatly reduced when the plants were grown under cool temperatures during seed maturation, but increased to varying degrees at higher temperatures. Sequence analysis of the γ-TMT3 promoter of 11 of the accessions identified four haplotypes, one of which corresponded to that of cultivars with higher contents. These wild accessions can thus serve as novel donors for breeding cultivars with high α-tocopherol ratios and for better understanding the genetic basis of α-tocopherol synthesis in soybean.

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“…Southern blot analysis was carried out according to the procedure described by Zhang et al . (), with slight modifications. The probe were radio‐labeled with [ 32 P]‐dCTP using the Prime‐a‐Gene® Labeling System (Promega).…”

Section: Methodsmentioning

confidence: 99%

“…Genomic DNA was extracted from S. pombe cultures using a zymolyase-based cell lysis and chromosomal DNA purification protocol previously described by Moreno et al (1991). Southern blot analysis was carried out according to the procedure described by Zhang et al (2013), with slight modifications. The probe were radio-labeled with [ 32 P]-dCTP using the Prime-a-Gene® Labeling System (Promega).…”

Section: Southern Blot Analysismentioning

confidence: 99%

A novel protein, Rsf1/Pxd1, is critical for the single‐strand annealing pathway of double‐strand break repair in Schizosaccharomyces pombe

Wang

Zhang

et al. 2015

Molecular Microbiology

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SummaryThe process of single-strand annealing (SSA) repairs DNA double-strand breaks that are flanked by direct repeat sequences through the coordinated actions of a series of proteins implicated in recombination, mismatch repair and nucleotide excision repair (NER). Many of the molecular and mechanistic insights gained in SSA repair have principally come from studies in the budding yeast Saccharomyces cerevisiae. However, there is little molecular understanding of the SSA pathway in the fission yeast Schizosaccharomyces pombe. To further our understanding of this important process, we established a new chromosome-based SSA assay in fission yeast. Our genetic analyses showed that, although many homologous components participate in SSA repair in these species indicating that some evolutionary conservation, Saw1 and Slx4 are not principal agents in the SSA repair pathway in fission yeast. This is in marked contrast to the function of Saw1 and Slx4 in budding yeast. Additionally, a novel genus-specific protein, Rsf1/Pxd1, physically interacts with Rad16, Swi10 and Saw1 in vitro and in vivo. We find that Rsf1/Pxd1 is not required for NER and demonstrate that, in fission yeast, Rsf1/Pxd1, but not Saw1, plays a critical role in SSA recombination.

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GmTMT2a from soybean elevates the α-tocopherol content in corn and Arabidopsis

Cited by 22 publications

References 27 publications

Natural variations in OsγTMT contribute to diversity of the α-tocopherol content in rice

Natural variations in OsγTMT contribute to diversity of the α-tocopherol content in rice

Natural diversity of seed α-tocopherol ratio in wild soybean (<i>Glycine soja</i>) germplasm collection

A novel protein, Rsf1/Pxd1, is critical for the single‐strand annealing pathway of double‐strand break repair in Schizosaccharomyces pombe

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