GnRH-GnRH-Receptor System in the Mammalian Female Reproductive Tract

Chowdhury, Indrajit; Sridaran, R.

doi:10.1007/978-0-387-88186-7_13

Cited by 2 publications

(4 citation statements)

References 36 publications

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“…In humans, melatonin treatment enhances the human chorionic gonadotropin (hCG)-stimulated progesterone secretion with inhibition of GnRH and GnRH receptor expression. In support of this, melatonin reduces both GnRH-I and GnRHR-I mRNA levels in human primary granulosa-luteal cells in a dose-dependent manner [ 28 ].…”

Section: Discussionmentioning

confidence: 99%

“…However, the reduction of GnRHR gene expression during inflammation in the PT may also result from the direct action of pro-inflammatory cytokines and stress, because both IL-1β and corticotropin-releasing hormone (CRH) were shown to suppress GnRHR expression [ 14 , 38 , 39 ]. Moreover, the lowered GnRHR expression in the pituitary cells which is observed in the inflammatory response may result also from the direct action of the bacterial endotoxin, as toll-like receptor (TLR) 4 recognizing LPS is present in these cells [ 28 , 40 , 41 ]. It was previously reported that treatment with LPS decreased GnRHR gene expression as well as GnRH-induced secretion of LH in ovine AP explants [ 29 ].…”

Section: Discussionmentioning

confidence: 99%

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Endotoxin-Induced Inflammation Suppresses the Effect of Melatonin on the Release of LH from the Ovine Pars Tuberalis Explants—Ex Vivo Study

2017

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The secretion of the hormone melatonin reliably reflects environmental light conditions. Among numerous actions, in seasonal breeders, melatonin may regulate the secretion of the gonadotropins acting via its corresponding receptors occurring in the Pars Tuberalis (PT). However, it was previously found that the secretory activity of the pituitary may be dependent on the immune status of the animal. Therefore, this study was designed to determine the role of melatonin in the modulation of luteinizing hormone (LH) secretion from the PT explants collected from saline- and endotoxin-treated ewes in the follicular phase of the oestrous cycle. Twelve Blackhead ewes were sacrificed 3 h after injection with lipopolysaccharide (LPS; 400 ng/kg) or saline, and the PTs were collected. Each PT was cut into 4 explants, which were then divided into 4 groups: I, incubated with ‘pure’ medium 199; II, treated with gonadotropin-releasing hormone (GnRH) (100 pg/mL); III, treated with melatonin (10 nmol/mL); and IV, incubated with GnRH and melatonin. Melatonin reduced (p < 0.05) GnRH-induced secretion of LH only in the PT from saline-treated ewes. Explants collected from LPS-treated ewes were characterized by lower (p < 0.05) GnRH-dependent response in LH release. It was also found that inflammation reduced the gene expression of the GnRH receptor and the MT1 melatonin receptors in the PT. Therefore, it was shown that inflammation affects the melatonin action on LH secretion from the PT, which may be one of the mechanisms via which immune/inflammatory challenges disturb reproduction processes in animals.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Endotoxin-Induced Inflammation Suppresses the Effect of Melatonin on the Release of LH from the Ovine Pars Tuberalis Explants—Ex Vivo Study

2017

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“…In comparison between experimental groups, estradiol IHC expression density in ovaries of SFBFF group female rats showed mild elevation (P>0.05) at proestrus phase of estrus cycle Figure (1) there is no significant changes (p>0.05) between experiment groups Table (4). In comparison between phases of the estrus cycle, in all experimental groups, the highest estradiol expression density has been reported at estrus and metestrus phases followed by proestrus phase, whereas the lowest densities have been reported at diestrus phase Figure (4).…”

Section: Ovary Estradiol Ihc Expressionmentioning

confidence: 93%

“…Rodent reproduction is regulated by accurate interactions throughout the hypothalamic-pituitary-gonadal axis, where the hypothalamus discharges matched pulses of GnRH as the primary inducer of the reproductive hormones secretion. GnRH stimulates the biosynthesis of pituitary LH and FSH that in turn promote gonadal gametogenesis and hormones secretion, as the key coordinator of the reproduction (1). LH and FSH are responsible for stimulating ovarian folliculogenesis as greater than four layers of granulosa cells as well as steroidogenesis, and ovulation (2).…”

Section: Introductionmentioning

confidence: 99%

Ovarian immunohistochemical expression of estradiol 17β in cyclic female rats treated with steroid free bovine follicular fluid antiserum

A.A.Al-Sa'aidi

Khafaji

2018

QJVMS

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The current study aimed to examine the effect of steroid-free bovine follicular fluid (SFBFF) antiserum on ovarian immunohistochemical expression levels of 17β Estradiol in cyclic virgin female rats. After estrus synchronization, 80 adult virgin female Wistar rats (aged 60 days and weighed 156±4.82 g) were randomly assigned into two equal groups (40 females each) and injected intrperitoneally, at late metestrous, with 100 µl of normal saline (control) and 100 µl of SFBFF antiserum (AI-SFBFF group). At each phase of the estrus cycle, 10 females from each group were anesthetized (by injection of 0.4 ml of thiopental sod./ animal), dissected and the ovaries were obtained for determination of immunohistochemical expression density of 17β Estradiol. Ovarian examination of AI-SFBFF group females demonstrated slight increase of estradiol immunohistochemical density at proestrus and mark increase at estrus and metestrus phases. It could be concluded that immunoneutralization of endogenous inhibin by SFBFF antiserum results in high levels of estradiol actions in reproductive organs.

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GnRH-GnRH-Receptor System in the Mammalian Female Reproductive Tract

Cited by 2 publications

References 36 publications

Endotoxin-Induced Inflammation Suppresses the Effect of Melatonin on the Release of LH from the Ovine Pars Tuberalis Explants—Ex Vivo Study

Endotoxin-Induced Inflammation Suppresses the Effect of Melatonin on the Release of LH from the Ovine Pars Tuberalis Explants—Ex Vivo Study

Ovarian immunohistochemical expression of estradiol 17β in cyclic female rats treated with steroid free bovine follicular fluid antiserum

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