GnRH in the male dog: dose-response relationships with LH and testosterone

Knol, B. W.; Dieleman, S.J.; Bevers, M.M.; Brom, W. E. van den

doi:10.1530/jrf.0.0980159

Cited by 24 publications

(30 citation statements)

References 4 publications

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“…Only in one dog, the level of plasma testosterone reached level of 1i ng/ml in 60 min and retumed baseline level in 180 min. The individual (Figure 1 In male dog, the reported normal testosterone range is 0.4 to 10.0 ng/ml, although some researchers (3,6,8) (9) reported that the plasma T levels of normal adult dogs peaked 60 and 90 min after an intravenous injection of LH-RH-A. Thcir results are similar those obtained in the present study.…”

Section: Resultssupporting

confidence: 87%

THE EFFECTS OF EXOGENE GnRH ON PLASMA TESTOSTERONE CONCENTRATIONS IN ANATOLIAN SHEPHERD DOG

Baştan¹,

Taşal²,

Kaymaz³

et al. 1996

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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Bu çalışmanın amacı erkek safkan Anadolu çoban köpeklerinde GnRH uygulamasından önce ve sonra kan testosteron konsantrasyonunu incelemek ve libidoyu uyarmadaki etkinliğini ortaya koymaktır. Bir GnRH analoğu olan Buserelin, 2-5 yaşlı 6 köpeğe 25 /Lg dozunda kas ıçı yol ile uygulandı. GnRH uygulamasından önce ve 1, 2 ve 3 saat sonra periferal plazma testosteron konsantrasyonu (T) radioimmunoassay ile saptandı. GnRH enjehiyonundan önce plazma T seviyesi 2.4:1:1.6 ng/ml idi.

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Section: Resultssupporting

confidence: 87%

THE EFFECTS OF EXOGENE GnRH ON PLASMA TESTOSTERONE CONCENTRATIONS IN ANATOLIAN SHEPHERD DOG

Baştan¹,

Taşal²,

Kaymaz³

et al. 1996

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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“…No data have been reported on the response of the plasma testosterone concentration to GnRH stimulation in the bitch. In intact male dogs administration of 10 μ g/kg GnRH results in a maximum testosterone concentration at 60 min after injection (Knol et al. 1993), and therefore we assumed that a GnRH‐induced increase in plasma testosterone concentration will be found in blood samples at 60 and 90 min after GnRH administration.…”

Section: Discussionmentioning

confidence: 99%

Effects of Gonadotrophin Releasing Hormone Administration on the Pituitary‐Ovarian Axis in Anoestrous vs Ovariectomized Bitches

Buijtels¹,

Beijerink²,

Kooistra³

et al. 2006

Reprod Domestic Animals

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ContentsThe aim of this study was to determine the effects of gonadotrophin releasing hormone (GnRH) administration on the plasma concentrations of reproductive hormones in intact and ovariectomized (OVX) bitches. Therefore, blood samples were collected at multiple times before and after the administration of 10 lg/kg GnRH (FertagylÒ) for the determination of the plasma concentrations of luteinizing hormone (LH), oestradiol, progesterone and testosterone in six anoestrus and in six OVX bitches. The mean plasma LH concentrations before and 60 min after GnRH administration were significantly lower in the anoestrous bitches than in the OVX bitches. In both groups GnRH administration resulted in a significant increase in the plasma LH concentration. The highest plasma LH concentrations were found at 10 min after GnRH administration and these values did not differ significantly between the two groups. Only in the anoestrous bitches a significant increase in plasma oestradiol concentrations was found after GnRH administration and these values were significantly higher than those in the OVX bitches. The plasma concentrations of progesterone and testosterone were low (close to or below the limit of quantitation) both before and after GnRH administration and the differences between anoestrous and OVX bitches were not significant. It can be concluded that (i) basal plasma LH concentration is significantly higher in OVX bitches than in anoestrous bitches, (ii) plasma LH concentration increases after GnRH administration in both anoestrous and OVX bitches, (iii) GnRH administration causes a significant rise in plasma oestradiol concentration only if ovarian tissue is present and (iv) measurement of plasma progesterone and testosterone concentrations before and after GnRH administration does not aid in distinguishing between anoestrous and OVX bitches. The results of this study may provide a basis for the diagnosis of remnant ovarian tissue and verification of neuter status in the bitch.

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“…Intra-and interassay coefficients of variation were 5·1% and 11·5%, respectively, and the sensitivity of the assay was 0·2 ng/ml. Plasma LH concentration was measured by a heterologous RIA as described by Knol et al (1993). A sheep antibody, CSU-204 (a gift from Dr Niswender, Colorado State University, Fort Collins, CO, USA), radioiodinated NIAMDD-bLH-4 and canine pituitary standard LER 1685-1 were used in this assay.…”

Section: Hormone Measurementsmentioning

confidence: 99%

Ageing, testicular tumours and the pituitary-testis axis in dogs

Peters

Jong²,

Teerds³

et al. 2000

Journal of Endocrinology

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Dogs of different ages without testicular diseases were evaluated to study possible age-related changes in hormone concentrations in serum. Dogs with testicular tumours were also investigated to study the relation between tumour type and hormone concentrations; in this study, dogs with Sertoli cell tumours, Leydig cell tumours and seminomas were included. We measured testosterone, oestradiol, LH, FSH and inhibin-like immunoreactivity concentrations in peripheral venous and testicular venous blood of these animals.In normal dogs there appeared to be no age-related changes in the concentrations of the investigated hormones, except for a significant age-related decrease in oestradiol concentrations in testicular venous blood (P<0·02). Dogs with a Sertoli cell tumour had greater oestradiol concentrations and inhibin-like immunoreactivity in both peripheral and testicular venous blood than did dogs without a neoplasm (P<0·05). Testosterone concentrations were reduced in dogs with Sertoli cell tumours, as were FSH and LH. Feminisation occurred in eight of 13 dogs with a Sertoli cell tumour and in two of 14 dogs with a Leydig cell tumour; it was accompanied by a significantly greater oestradiol concentration than in normal dogs and in dogs with Sertoli cell tumours without signs of feminisation. Dogs with a Leydig cell tumour had greater concentrations of oestradiol and inhibin-like immunoreactivity in both peripheral venous and testicular venous blood than did dogs without a neoplasm (P<0·05). The testosterone concentration in testicular venous blood of these dogs was lower than that in dogs with normal testes. The concentration of LH in peripheral venous blood was also reduced (P<0·05). Hormone concentrations in dogs with a seminoma were not different from those in normal dogs.It was concluded that seminomas are not endocrinologically active. In contrast, both Sertoli cell tumours and Leydig cell tumours can cause increased oestrogen production leading to signs of feminisation. These tumours also have considerable amounts of inhibin-like immunoreactivity, but only in Sertoli cell tumours does this result in a reduction in FSH concentrations, suggesting that Sertoli cell tumours secrete dimeric inhibin, whereas Leydig cell tumours presumably produce loose -subunits that cross-react in the inhibin assay but are not biologically active.

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GnRH in the male dog: dose-response relationships with LH and testosterone

Cited by 24 publications

References 4 publications

THE EFFECTS OF EXOGENE GnRH ON PLASMA TESTOSTERONE CONCENTRATIONS IN ANATOLIAN SHEPHERD DOG

THE EFFECTS OF EXOGENE GnRH ON PLASMA TESTOSTERONE CONCENTRATIONS IN ANATOLIAN SHEPHERD DOG

Effects of Gonadotrophin Releasing Hormone Administration on the Pituitary‐Ovarian Axis in Anoestrous vs Ovariectomized Bitches

Ageing, testicular tumours and the pituitary-testis axis in dogs

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