Goblet cell hyperplasia as a feature of neutrophilic asthma

Alagha, Khuder; Bourdin, Arnaud; Vernisse, Charlotte; Garulli, Céline; Tummino, C.; Charriot, Jeremy; Vachier, Isabelle; Suehs, Carey M.; Chanez, Pascal; Gras, Delphine

doi:10.1111/cea.13359

Cited by 19 publications

(9 citation statements)

References 39 publications

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“…Goblet cell differentiation and excessive mucus production are common features of many airway diseases (19) . Patients with COPD and asthma exhibit increased goblet cell hyperplasia and luminal mucin production (20)(21)(22) . MUC5AC levels are increased by goblet cell hyperplasia and metaplasia in sinus mucosa of patients with chronic rhinosinusitis (23,24) .…”

Section: Discussionmentioning

confidence: 99%

Fipronil upregulates inflammatory cytokines and MUC5AC expression in human nasal epithelial cells

Kwak

Choi

et al. 2019

Rhin

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Background: Airway inflammation and excessive mucin production are pathophysiological characteristics of airway diseases.Fipronil, a pesticide, is being extensively used in agriculture and veterinary medicine worldwide. However, this compound impairs immune function in non-target organisms. The present study aimed to evaluate the effect of fipronil on pro-inflammatory cytokine and mucus production and signalling pathways in human primary nasal epithelial cells. Methodology:The effect of fipronil on pro-inflammatory cytokine and MUC5AC expression and the signalling pathway of fipronil were investigated using real-time PCR, enzyme immunoassays, immunofluorescence, and immunoblot analysis with specific inhibitors and small interfering RNA.Results: Fipronil treatment increased pro-inflammatory cytokine interleukin (IL)-1β, IL-6, IL-8, and MUC5AC expression in human primary nasal epithelial cells. It also induced phosphorylation of extracellular signal-regulated kinase 1/2 (ERK1/2) mitogenactivated protein kinase (MAPK), p38 MAPK, and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB). MAPK and NF-kB inhibitor treatment significantly inhibited increases in IL-1β, IL-6, IL-8, and MUC5AC expression. Ex vivo data confirmed that fipronil-induced MUC5AC expression occurs through ERK1/2, p38, and NF-κB signalling pathways in nasal inferior turbinate tissue.Conclusions: Fipronil induced pro-inflammatory cytokine IL-1β, IL-6, IL-8, and MUC5AC expression via ERK1/2 MAPK, p38 MAPK, and NF-κB in human primary nasal epithelial cells.

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Section: Discussionmentioning

confidence: 99%

Fipronil upregulates inflammatory cytokines and MUC5AC expression in human nasal epithelial cells

Kwak

Choi

et al. 2019

Rhin

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“…In patients with asthma, tracheal goblet cells undergo hyperplasia and secrete excess mucus that obstructs the airway [ 20 ]. Mucins are glycoproteins produced by airway epithelial cells to clear allergens or microorganisms that invade the respiratory system [ 21 ]. In double IL-4/IL-13 knockout mice, the induction of asthma did not promote tracheal goblet cell hyperplasia and mucin secretion [ 22 ].…”

Section: Discussionmentioning

confidence: 99%

Gypenoside A from Gynostemma pentaphyllum Attenuates Airway Inflammation and Th2 Cell Activities in a Murine Asthma Model

Huang

Yeh

et al. 2022

IJMS

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Our previous study found that oral administration of Gynostemma pentaphyllum extract can attenuate airway hyperresponsiveness (AHR) and reduce eosinophil infiltration in the lungs of asthmatic mice. Gypenoside A is isolated from G. pentaphyllum. In this study, we investigated whether gypenoside A can effectively reduce asthma in mice. Asthma was induced in BALB/c mice by ovalbumin injection. Asthmatic mice were treated with gypenoside A via intraperitoneal injection to assess airway inflammation, AHR, and immunomodulatory effects. In vitro, gypenoside A reduced inflammatory and oxidative responses in inflammatory tracheal epithelial cells. Experimental results showed that gypenoside A treatment can suppress eosinophil infiltration in the lungs, reduce tracheal goblet cell hyperplasia, and attenuate AHR. Gypenoside A significantly reduced Th2 cytokine expression and also inhibited the expression of inflammatory genes and proteins in the lung and bronchoalveolar lavage fluid. In addition, gypenoside A also significantly inhibited the secretion of inflammatory cytokines and chemokines and reduced oxidative expression in inflammatory tracheal epithelial cells. The experimental results suggested that gypenoside A is a natural compound that can effectively reduce airway inflammation and AHR in asthma, mainly by reducing Th2 cell activation.

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“…Validated questionnaires have been developed to obtain semi-quantitative scales that can be used in clinical trials and daily clinical practice. The Cough and Sputum Assessment Questionnaire (CASA-Q), mostly used in COPD and asthma, is very specific to chronic bronchitis and has the advantage of assessing both symptom intensity and their effects [ 138 , 139 , 140 ]. Strikingly, Alagha et al showed that chronic bronchitis assessed by CASA-Q was not correlated with goblet cell hyperplasia in asthma, calling into question the reliability of such clinical evaluation.…”

Section: Indirect Assessment Of Human Mucus Dysregulationmentioning

confidence: 99%

Methods of Sputum and Mucus Assessment for Muco-Obstructive Lung Diseases in 2022: Time to “Unplug” from Our Daily Routine!

Charriot

Volpato

Petit

et al. 2022

Cells

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Obstructive lung diseases, such as chronic obstructive pulmonary disease, asthma, or non-cystic fibrosis bronchiectasis, share some major pathophysiological features: small airway involvement, dysregulation of adaptive and innate pulmonary immune homeostasis, mucus hyperproduction, and/or hyperconcentration. Mucus regulation is particularly valuable from a therapeutic perspective given it contributes to airflow obstruction, symptom intensity, disease severity, and to some extent, disease prognosis in these diseases. It is therefore crucial to understand the mucus constitution of our patients, its behavior in a stable state and during exacerbation, and its regulatory mechanisms. These are all elements representing potential therapeutic targets, especially in the era of biologics. Here, we first briefly discuss the composition and characteristics of sputum. We focus on mucus and mucins, and then elaborate on the different sample collection procedures and how their quality is ensured. We then give an overview of the different direct analytical techniques available in both clinical routine and more experimental settings, giving their advantages and limitations. We also report on indirect mucus assessment procedures (questionnaires, high-resolution computed tomography scanning of the chest, lung function tests). Finally, we consider ways of integrating these techniques with current and future therapeutic options. Cystic fibrosis will not be discussed given its monogenic nature.

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Goblet cell hyperplasia as a feature of neutrophilic asthma

Abstract: Background:Goblet cell hyperplasia (GCH) is a pathological finding classically re-

Cited by 19 publications

References 39 publications

Fipronil upregulates inflammatory cytokines and MUC5AC expression in human nasal epithelial cells

Fipronil upregulates inflammatory cytokines and MUC5AC expression in human nasal epithelial cells

Gypenoside A from Gynostemma pentaphyllum Attenuates Airway Inflammation and Th2 Cell Activities in a Murine Asthma Model

Methods of Sputum and Mucus Assessment for Muco-Obstructive Lung Diseases in 2022: Time to “Unplug” from Our Daily Routine!

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