Gold-iron oxide nanohybrids: insights into colloidal stability and surface-enhanced Raman detection

Schwaminger, Sebastian P.; Bauer, David; Fraga-García, Paula

doi:10.1039/d1na00455g

Cited by 11 publications

(12 citation statements)

References 63 publications

(126 reference statements)

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“…The surfaces of MNPs for in vivo applications should be modified with a highly biocompatible material to acquire excellent stability, good solubility, and low toxicity [ 1 ]. The good magnetic core surface protection and low interaction with a solvent primarily lead to high biocompatibility of MNPs [ 2 , 3 , 31 , 32 , 33 , 34 ]. The core–shell structure of MNCs is generally well tolerated in vivo [ 25 , 35 , 36 , 37 , 38 ].…”

Section: Introductionmentioning

confidence: 99%

pH-Responsible Doxorubicin-Loaded Fe3O4@CaCO3 Nanocomposites for Cancer Treatment

2023

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A magnetic nanocomposite (MNC) is an integrated nanoplatform that combines a set of functions of two types of materials. A successful combination can give rise to a completely new material with unique physical, chemical, and biological properties. The magnetic core of MNC provides the possibility of magnetic resonance or magnetic particle imaging, magnetic field-influenced targeted delivery, hyperthermia, and other outstanding applications. Recently, MNC gained attention for external magnetic field-guided specific delivery to cancer tissue. Further, drug loading enhancement, construction stability, and biocompatibility improvement may lead to high progress in the area. Herein, the novel method for nanoscale Fe3O4@CaCO3 composites synthesis was proposed. For the procedure, oleic acid-modified Fe3O4 nanoparticles were coated with porous CaCO3 using an ion coprecipitation technique. PEG-2000, Tween 20, and DMEM cell media was successfully used as a stabilization agent and template for Fe3O4@CaCO3 synthesis. Transmission electron microscopy (TEM), Fourier transform infrared (FTIR) spectroscopy, and dynamic light scattering (DLS) data were used for the Fe3O4@CaCO3 MNC’s characterization. To improve the nanocomposite properties, the concentration of the magnetic core was varied, yielding optimal size, polydispersity, and aggregation ability. The resulting Fe3O4@CaCO3 had a size of 135 nm with narrow size distributions, which is suitable for biomedical applications. The stability experiment in various pH, cell media, and fetal bovine serum was also evaluated. The material showed low cytotoxicity and high biocompatibility. An excellent anticancer drug doxorubicin (DOX) loading of up to 1900 µg/mg (DOX/MNC) was demonstrated. The Fe3O4@CaCO3/DOX displayed high stability at neutral pH and efficient acid-responsive drug release. The series of DOX-loaded Fe3O4@CaCO3 MNCs indicated effective inhibition of Hela and MCF-7 cell lines, and the IC 50 values were calculated. Moreover, 1.5 μg of the DOX-loaded Fe3O4@CaCO3 nanocomposite is sufficient to inhibit 50% of Hela cells, which shows a high prospect for cancer treatment. The stability experiments for DOX-loaded Fe3O4@CaCO3 in human serum albumin solution indicated the drug release due to the formation of a protein corona. The presented experiment showed the “pitfalls” of DOX-loaded nanocomposites and provided step-by-step guidance on efficient, smart, anticancer nanoconstruction fabrication. Thus, the Fe3O4@CaCO3 nanoplatform exhibits good performance in the cancer treatment area.

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Section: Introductionmentioning

confidence: 99%

pH-Responsible Doxorubicin-Loaded Fe3O4@CaCO3 Nanocomposites for Cancer Treatment

2023

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“…For SO concentrations above a certain value, the critical micelle concentration (CMC), the molecules organize themselves in aqueous media and form micelles. The CMC of SO is highly dependent on pH and temperature; values from 0.2 to 2.7 mM have been reported in the literature. ,, This surfactant (the acid or the salt) is widely used to coat bare iron oxide nanoparticles, ,, as it can stabilize them in solution due to electrostatic repulsions, thus reducing the size of the nanoparticle agglomerates . Dextran is a neutral hydrophilic polysaccharide, with a linear glucose chain linked by α-D-(1–6) linkages, with 5% of α-(1–3) branch units.…”

Section: Introductionmentioning

confidence: 99%

“…31,46,47 This surfactant (the acid or the salt) is widely used to coat bare iron oxide nanoparticles, 31,48,49 as it can stabilize them in solution due to electrostatic repulsions, thus reducing the size of the nanoparticle agglomerates. 50 Dextran is a neutral hydrophilic polysaccharide, with a linear glucose chain linked by α-D-(1− 6) linkages, with 5% of α-(1−3) branch units. It is one of the typical polymers used as a coating in nanoparticles, due to its good biocompatibility.…”

Section: Introductionmentioning

confidence: 99%

Competition at the Bio-nano Interface: A Protein, a Polysaccharide, and a Fatty Acid Adsorb onto Magnetic Nanoparticles

Abarca-Cabrera

Berensmeier

et al. 2022

ACS Appl. Bio Mater.

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Magnetic nanoparticles are an attractive bioseparation tool due to their magnetic susceptibility and high adsorption capacity for different types of molecules. A major challenge for separation is to generate selectivity for a target molecule, or for a group of molecules in complex environments such as cell lysates. It is crucial to understand the factors that determine the targets’ adsorption behavior in mixtures for triggering intended interactions and selectivity. Here we use a model system containing three molecules, each of them a common representative of the more abundant types of macromolecules in living systems: sodium oleate (SO), a fatty acid; bovine serum albumin (BSA), a protein; and dextran, a polysaccharide. Our results show that (a) the BSA adsorption capacity on the iron oxide material depends markedly on the pH, with the maximum capacity at the pI of the protein (0.39 g gMNP -1 ); (b) sodium oleate, a strongly negatively charged molecule, an organic anion, renders a maximum adsorption capacity of 0.40 g gMNP -1, even at pHs at which oleate as well as the nanoparticle surface are negatively charged; (c) the adsorbed masses of dextran, a neutral sugar, are lower than for the other two molecules, between 0.09 and 0.13 g gMNP -1, regardless of the system’s pH. We observe an unexpected behavior in mixtures: SO completely prevents the adsorption of BSA, and dextran decreases the adsorption of the other competitors, SO and BSA, while adsorbing at the same capacities, unaffected by either the presence of the other two molecules or the pH. BSA does not decrease the oleate adsorption capacity. We demonstrate the essential role of pH in the adsorption of BSA (a protein) and SO (a fatty acid), as well as its impact in the structural organization of the oleate molecules in water. Moreover, we present exciting data on the adsorption of the molecules in competition, revealing the need to focus on interaction studies in more complex environments. This study attempts to open the scope of the current research of bio-nano interactions to not only proteins but also to mixtures, and generally to molecules with other physicochemical characteristics. Furthermore, we contribute to the understanding of multicomponent systems with the vision set in enhancing biomass exploitation and biofractionation processes.

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“…Schwaminger et al also calculated the size distribution similarly. They declared the size of 10 nm for the gold-coated magnetic NPs [26]. However, the provided TEM represent aggregated nanoparticles as a result of the magnetic force of the TEM experiment.…”

Section: Discussionmentioning

confidence: 99%

Radiosensitising effect of iron oxide‐gold nanocomplex for electron beam therapy of melanoma in vivo by magnetic targeting

Mohamadkazem

Neshasteh-Riz

Amini

et al. 2023

IET Nanobiotechnology

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Melanoma is a dangerous type of skin cancer sometimes treated with radiotherapy. However, it induces damage to the surrounding healthy tissue and possibly further away areas. Therefore, it is necessary to give a lower dose to the patient with targeted therapy. In this study, the radio‐sensitising effect of gold‐coated iron oxide nanoparticles on electron beam radiotherapy of a melanoma tumour with magnetic targeting in a mouse model was investigated. Gold‐coated iron oxide nanoparticles were prepared in a steady procedure. The melanoma tumour model was induced in mice. Animals were divided into five groups: (1) normal; (2) melanoma; (3) gold‐coated iron oxide nanoparticles alone; (4) electron beam radiotherapy; (5) electron beam radiotherapy plus gold‐coated iron oxide nanoparticles. The magnet was placed on the tumour site for 2 h. The tumours were then exposed to 6 MeV electron beam radiotherapy for a dose of 8 Gy. Inductively coupled plasma optical emission spectrometry test, hematoxylin and eosin staining, and enzyme‐linked immunosorbent assay blood test were also performed. Gold‐coated iron oxide nanoparticles with magnetic targeting before electron beam radiotherapy reduced the growth of the tumour compared to the control group. Blood tests did not show any significant toxicity. Deposition of nanoparticles was more in the tumour and spleen tissue and to a lesser extent in the liver, kidney, and lung tissues. The synergistic effect of nanoparticles administered by the intraperitoneal route and then concentrated into the tumour area by application of an external permanent magnet, before delivery of the electron beam radiotherapy improved the overall cancer treatment outcome and prevented metal distribution side effects.

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Gold-iron oxide nanohybrids: insights into colloidal stability and surface-enhanced Raman detection

Abstract: Nanoparticles are acquiring an ever increasing role in analytical technologies for enhanced applications such as signalling of hazardous dyes. One challenge for the synthesis of hybrid nanomaterials is to control...

Cited by 11 publications

References 63 publications

pH-Responsible Doxorubicin-Loaded Fe3O4@CaCO3 Nanocomposites for Cancer Treatment

pH-Responsible Doxorubicin-Loaded Fe3O4@CaCO3 Nanocomposites for Cancer Treatment

Competition at the Bio-nano Interface: A Protein, a Polysaccharide, and a Fatty Acid Adsorb onto Magnetic Nanoparticles

Radiosensitising effect of iron oxide‐gold nanocomplex for electron beam therapy of melanoma in vivo by magnetic targeting

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