Gold nanocatalyst-based immunosensing strategy accompanying catalytic reduction of 4-nitrophenol for sensitive monitoring of chloramphenicol residue

Que, Xiaohua; Tang, Dianyong; Xia, Biyun; Lu, Minghua; Tang, Dianping

doi:10.1016/j.aca.2014.04.051

Cited by 35 publications

(23 citation statements)

References 37 publications

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“…19,22 As another example, AuNPs can catalyze the reduction of organic compounds like 4-nitrophenol, 23 which has been used for the detection of different proteins. 24,25 But this catalytic reduction is only limited to several colored substances, including methyl orange, 26 4-nitrophenol 27 and methylene blue. 18 Thus, the development of additional simple and rapid analytical methods with extraordinary sensitivity is highly desirable.…”

Section: Introductionmentioning

confidence: 99%

Gold nanoparticle-catalyzed uranine reduction for signal amplification in fluorescent assays for melamine and aflatoxin B1

Wang

Pauli

Nießner

et al. 2015

Analyst

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A multifunctional fluorescence platform has been constructed based on gold nanoparticle (AuNP)-catalyzed uranine reduction. The catalytic reduction of uranine was conducted in aqueous solution using AuNPs as nanocatalyst and sodium borohydride as reducing reagent, which was monitored by fluorescence and UV-vis spectroscopy. The reaction rate was highly dependent on the concentration, size and dispersion state of AuNPs. When AuNPs aggregated, their catalytic ability decreased, and thereby a label-free fluorescent assay was developed for the detection of melamine, which can be used for melamine determination in milk. In addition, a fluorescent immunoassay for aflatoxin B1 (AFB1) was established using the catalytic reaction for signal amplification based on target-induced concentration change of AuNPs, where AFB1-BSA-coated magnetic beads and anti-AFB1 antibody-conjugated AuNPs were employed as capture and signal probe, respectively. The detection can be accomplished in 1 h and acceptable recoveries in spiked maize samples were achieved. The developed fluorescence system is simple, sensitive and specific, which could be used for the detection of a wide range of analytes.

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Section: Introductionmentioning

confidence: 99%

Gold nanoparticle-catalyzed uranine reduction for signal amplification in fluorescent assays for melamine and aflatoxin B1

Wang

Pauli

Nießner

et al. 2015

Analyst

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“…It can emit light in two seconds and full automation. Therefore, it has been exploited in CLIAs to gain higher sensitivity for rapid screening in food safety detection [9].…”

Section: Introductionmentioning

confidence: 99%

Highly efficient and sensitive detection of chloramphenicol based on chemiluminescence immunoassays with functionalized Fe3O4@SiO2@Au magnetic nanoparticles

Wang¹,

Yao²,

Fang³

et al. 2016

Proceedings of the 2016 5th International Conference on Environment, Materials, Chemistry and Power Electronics

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To avoid the long time of dialysis process, a novel Strategy that Bovine serum albumin (BSA) was immobilized on Au-MNPs at first and afterward the CAP molecules were immobilized on the BSA in sequence has been applied to Chloramphenicol (CAP) detection with chemiluminescent immunoassays (CLIA) based on functionalized Fe 3 O 4 @SiO 2 @Au magnetic nanoparticles. More importantly, this designed method was successfully applied to CAP detection with the highly efficient and sensitive. With the functionalized gold magnetic nanoparticles (Au-MNPs). Samples were detected utilizing the two different chemiluminescence immunoassays, with limits of detection (LODs) of 0.07 pg mL-1 for Method I and 0.06 pg mL-1 for Method II and a 50% inhibition concentration (IC 50) value of 0.086 ng mL-1 for Method I and 0.14 ng mL-1 for Method II for CAP respectively. Therefore, such chemiluminence immunoassays method could be easily adapted for small molecule detection in a variety of foods using Au-MNPs to conventional ILESA immunoassays in CAP detection.

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“…1,2 Recent research studies have demonstrated that CAP is a suspected carcinogen which may cause fatal side effects such as aplastic anemia and hypersensitivity in humans, and even a low concentration of CAP may lead to seriously adverse effects. 1,2 Recent research studies have demonstrated that CAP is a suspected carcinogen which may cause fatal side effects such as aplastic anemia and hypersensitivity in humans, and even a low concentration of CAP may lead to seriously adverse effects.…”

Section: Introductionmentioning

confidence: 99%

A sensitive colorimetric aptasensor for chloramphenicol detection in fish and pork based on the amplification of a nano-peroxidase-polymer

et al. 2015

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A novel colorimetric aptasensor was developed for sensitive and selective determination of chloramphenicol (CAP) based on gold nanoparticles (AuNPs) labeled with Power Vision (PV) and magnetic separation. PV, with a high enzyme-to-antibody ratio, is composed of a compact enzymelinker antibody conjunction. In this assay, an aptamer of CAP was immobilized on Fe 3 O 4 @Au magnetic nanoparticles as a capture probe (AuMNPs-Apt) to concentrate target CAP. The complementary DNA (cDNA) and PV were both labeled on AuNPs to form a nano-peroxidase polymer as a signal tag (cDNA-AuNPs-PV). And the special tags could hybridize with the aptamer and cDNA to form AuMNP-Apt/cDNA-AuNP-PV conjugates. In the presence of CAP, the aptamer preferentially bound to CAP and caused the dissociation of some cDNA-AuNPs-PV on the conjugates with magnetic separation. PV, carried on signal tags, could greatly catalyze 3,3 0 ,5,5 0 -tetramethylbenzidine (TMB) leading to color development, which could be quantified by Ultraviolet-visible (UV-vis) spectroscopy. A linear response to CAP concentration in the range of 0.05-200 ng mL À1 was obtained by this proposed method, with a low detection limit down to 0.02 ng mL À1 . Besides, this assay was successfully employed to analyze CAP in fish and pork samples, whose results were consistent with those of the conventional enzyme-linked immunosorbent assay (ELISA) method.

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Gold nanocatalyst-based immunosensing strategy accompanying catalytic reduction of 4-nitrophenol for sensitive monitoring of chloramphenicol residue

Cited by 35 publications

References 37 publications

Gold nanoparticle-catalyzed uranine reduction for signal amplification in fluorescent assays for melamine and aflatoxin B1

Gold nanoparticle-catalyzed uranine reduction for signal amplification in fluorescent assays for melamine and aflatoxin B1

Highly efficient and sensitive detection of chloramphenicol based on chemiluminescence immunoassays with functionalized Fe3O4@SiO2@Au magnetic nanoparticles

A sensitive colorimetric aptasensor for chloramphenicol detection in fish and pork based on the amplification of a nano-peroxidase-polymer

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