Gold nanoparticle-based colorimetric sensing of dipicolinic acid from complex samples

Baig, Mirza Muhammad Fahad; Chen, Yu-Chie

doi:10.1007/s00216-017-0836-2

Cited by 23 publications

(12 citation statements)

References 59 publications

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“…The binding affinity was investigated by fluorescence and K D was calculated between 5 × 10 −8 to 1 × 10 −7 M. This strategy allowed B. cereus spore detection via dipicolinic acid quantification in a concentration of 10 4 CFU/mL. Baig and Chen have used glutathione-capped gold nanoparticles complexed with Ca 2+− ions (Ca 2+ -AuNP@GSH) through GSH-Ca 2+ chelation as a sensing agent for dipicolinic acid from complex matrices [83]. In the presence of dipicolinic, Ca 2+ -AuNP@GSH is dissociated to the Ca 2+ -dipicolinic complex and AuNP@GSH, which desegregates nanoparticles and induces a color change of the solution.…”

Section: Dipicolinic Acid Detectionmentioning

confidence: 99%

Food Sensing: Detection of Bacillus cereus Spores in Dairy Products

Vidić

Chaix

Manzano³

et al. 2020

Biosensors

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Milk is a source of essential nutrients for infants and adults, and its production has increased worldwide over the past years. Despite developments in the dairy industry, premature spoilage of milk due to the contamination by Bacillus cereus continues to be a problem and causes considerable economic losses. B. cereus is ubiquitously present in nature and can contaminate milk through a variety of means from the farm to the processing plant, during transport or distribution. There is a need to detect and quantify spores directly in food samples, because B. cereus might be present in food only in the sporulated form. Traditional microbiological detection methods used in dairy industries to detect spores show limits of time (they are time consuming), efficiency and sensitivity. The low level of B. cereus spores in milk implies that highly sensitive detection methods should be applied for dairy products screening for spore contamination. This review describes the advantages and disadvantages of classical microbiological methods used to detect B. cereus spores in milk and milk products, related to novel methods based on molecular biology, biosensors and nanotechnology.

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Section: Dipicolinic Acid Detectionmentioning

confidence: 99%

Food Sensing: Detection of Bacillus cereus Spores in Dairy Products

Vidić

Chaix

Manzano³

et al. 2020

Biosensors

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“…Table S1 summarizes some of the colorimetric analytical procedures for the determination of DPA. 21,32,33 It is noticeable that the present approach based on the UCNPs−TPP/EBT nanoprobe offers a favorable detection limit.…”

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confidence: 95%

Placeholder Strategy with Upconversion Nanoparticles−Eriochrome Black T Conjugate for a Colorimetric Assay of an Anthrax Biomarker

et al. 2019

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The timely warning of the germination of bacterial spores and their prevention are highly important to minimize their potential detrimental effects and for disease control. Thus, a sensitive and selective assay of biomarkers is most desirable. In this work, a nanoprobe is constructed by conjugating lanthanide upconversion nanoparticles (UCNPs) with sodium tripolyphosphate (TPP) and eriochrome black T (EBT). The nanoprobe, UCNPs−TPP/EBT, serves as a platform for the detection of the anthrax biomarker, dipicolinic acid (DPA). In principle, DPA displaces EBT from the UCNPs−TPP/EBT nanoconjugate, resulting in a color change from magenta to blue because of the release of free EBT into the aqueous solution. The binding sites on UCNPs are partly preblocked with TPP as the placeholder molecule, leaving a desired number of binding sites for EBT conjugation. On the basis of this dye displacement reaction, a novel colorimetric assay protocol for DPA is developed, deriving a linear calibration range from 2 to 200 μM with a detection limit of 0.9 μM, which is well below the infectious dose of the spores (60 μM). The assay platform exhibits excellent anti-interference capability when treating a real biological sample matrix. The present method is validated by the analysis of DPA in human serum, and its practical application is further demonstrated by monitoring the DPA release upon spore germination.

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“…In particular, lanthanide luminescence has become a powerful tool for sensing DPA due to its selectivity, sensitivity, straightforward and quick detection, and low cost [for europium (Eu) based sensors see references [ 40,48,50,51,56–72 ] ; for other lanthanide based DPA sensors see references [ 41,45,47,54,73–87 ] ; for other DPA sensors see references. [ 88–92 ]…”

Section: Introductionmentioning

confidence: 99%

Eu(III)–DO3A and BODIPY dyad as a chemosensor for anthrax biomarker

Alp

Algı

2021

Luminescence

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The sensitive and selective determination of Bacillus anthracis spores before the infection is vital for human health and safety. Dipicolinic acid (DPA) is an excellent biomarker due to its presence in the nucleus of bacterial spores at high concentrations (up to 1 M, about 15% dry weight). In the present work, a new molecular chemosensor 1, based on europium(III)–DO3A and BODIPY dyad, is developed to detect DPA in phosphate‐buffered saline (PBS) buffered solution and tap water samples. Also, 1 can be used as a ratiometric optical chemosensor to track DPA.

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Gold nanoparticle-based colorimetric sensing of dipicolinic acid from complex samples

Cited by 23 publications

References 59 publications

Food Sensing: Detection of Bacillus cereus Spores in Dairy Products

Food Sensing: Detection of Bacillus cereus Spores in Dairy Products

Placeholder Strategy with Upconversion Nanoparticles−Eriochrome Black T Conjugate for a Colorimetric Assay of an Anthrax Biomarker

Eu(III)–DO3A and BODIPY dyad as a chemosensor for anthrax biomarker

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