Gold nanoparticle fluorescent molecular beacon for low-resolution DQ2 gene HLA typing

Beni, Valerio; Zewdu, Taye; Joda, Hamdi; Katakis, Ioanis; O’Sullivan, Ciara K.

doi:10.1007/s00216-011-5493-2

Cited by 14 publications

(6 citation statements)

References 38 publications

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“…Beni et al [ 47 ] developed a turn-on fluorescence genosensor for the identification of HLA-DQ2 genes using molecular-beacon (MB)-functionalized AuNPs. MBs, consisting of a 17-nucleotide-long recognition sequence, a terminating thiol group, and a five-nucleotide stem with ten thymines between the functional elements of the MB, were especially designed for target recognition.…”

Section: Biosensors For CD Detectionmentioning

confidence: 99%

Biosensors for Non-Invasive Detection of Celiac Disease Biomarkers in Body Fluids

Pasinszki

Krebsz

2018

Biosensors

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Celiac disease is a chronic gluten-initiated autoimmune disorder that predominantly damages the mucosa of the small intestine in genetically-susceptible individuals. It affects a large and increasing number of the world’s population. The diagnosis of this disease and monitoring the response of patients to the therapy, which is currently a life-long gluten-free diet, require the application of reliable, rapid, sensitive, selective, simple, and cost-effective analytical tools. Celiac disease biomarker detection in full blood, serum, or plasma offers a non-invasive way to do this and is well-suited to being the first step of diagnosis. Biosensors provide a novel and alternative way to perform conventional techniques in biomarker sensing, in which electrode material and architecture play important roles in achieving sensitive, selective, and stable detection. There are many opportunities to build and modify biosensor platforms using various materials and detection methods, and the aim of the present review is to summarize developments in this field.

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Section: Biosensors For CD Detectionmentioning

confidence: 99%

Biosensors for Non-Invasive Detection of Celiac Disease Biomarkers in Body Fluids

Pasinszki

Krebsz

2018

Biosensors

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“…Similarly, Beni et al used Au-nanobeacons to successfully detect a mutation that occurs in 70 % of cystic fibrosis patients using nM concentrations of DNA target [64,65]. In the second case, AuNPs can be combined with dye-labeled ssDNA probe and is used to detect specific DNA targets mediated by energy transfer mechanisms (FRET).…”

Section: Gold Nanoparticles Modulation Of Fluorescencementioning

confidence: 99%

Gold Nanoparticles for DNA/RNA-Based Diagnostics

Franco¹,

Pedrosa²,

Carlos³

et al. 2015

Handbook of Nanoparticles

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The remarkable physicochemical properties of gold nanoparticles (AuNPs) have prompted development in exploring biomolecular interactions with AuNPs-containing systems, pursuing biomedical applications in diagnostics. Among these applications, AuNPs have been remarkably useful for the development of DNA/RNA detection and characterization systems for diagnostics, including systems suitable for point of need. Here, emphasis will be on available molecular detection schemes of relevant pathogens and their molecular characterization, genomic sequences associated with medical conditions (including cancer), mutation and polymorphism identification, and the quantification of gene expression.

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“…1 The structural change can be monitored by conjugating a fluorophore–quencher pair to the SLP probe such that the binding of target nucleic acid to the loop region results in the separation of fluorophore– quencher pair resulting in the emission of fluorescence, which can be correlated with the concentration of nucleic acid target. This classical SLP and its various modifications have been employed in nucleic acid detection for a variety of applications including nucleic acid hybridization, 1–3 real time polymerase chain reactions (RT-PCR), 4,5 RNA hybridization in living cells, 6,7 and DNA-protein interaction. 8 Beside the classical design of SLP with fluorophore-quencher pair, different labels have been reported to improve sensitivity of SLP-based assays.…”

Section: Introductionmentioning

confidence: 99%

Design of Gaussia luciferase-based bioluminescent stem-loop probe for sensitive detection of HIV-1 nucleic acids

Joda

Moutsiopoulou

Stone

et al. 2018

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Here we describe the design of a bioluminescent stem-loop probe for the sensitive detection of HIV-1 spliced RNA. In this study, we employed Gaussia luciferase (GLuc), a bioluminescent protein that has several advantages over other bioluminescent proteins, including smaller size, higher bioluminescent intensity, and chemical and thermal stability. GLuc was chemically conjugated to the DABCYL-modified stem-loop probe (SLP) and was purified with a 2-step process to remove unconjugated GLuc and SLP. The binding of the target RNA to the loop region of the SLP results in the open conformation separating the stem part of SLP. GLuc conjugated to the stem acts as a reporter that produces light by a chemical reaction upon adding its substrate, coelenterazine in the presence of the target, while DABCYL serves as a quencher of bioluminescence in the closed conformation of SLP in the absence of the target. The optimized GLuc based-SLP assay resulted in a signal-to-background ratio of 47, which is the highest reported with bioluminescent SLPs and is significantly higher compared to traditional fluorescence-based SLPs that yield low signal to background ratio. Moreover, the assay showed an excellent selectivity against a single and double mismatched nucleic acid target, low detection limit, and ability to detect spiked HIV-1 RNA in human serum matrix.

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Gold nanoparticle fluorescent molecular beacon for low-resolution DQ2 gene HLA typing

Cited by 14 publications

References 38 publications

Biosensors for Non-Invasive Detection of Celiac Disease Biomarkers in Body Fluids

Biosensors for Non-Invasive Detection of Celiac Disease Biomarkers in Body Fluids

Gold Nanoparticles for DNA/RNA-Based Diagnostics

Design of Gaussia luciferase-based bioluminescent stem-loop probe for sensitive detection of HIV-1 nucleic acids

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