Gold Nanoparticle-Mediated Delivery of Therapeutic Enzymes for Biomedical Applications

Verma, Madan L.; Kumar, Pankaj; Sharma, Sneh; Dhiman, Karuna; Sharma, D. R.; Verma, Aruna

doi:10.1007/978-3-030-29207-2_3

Cited by 5 publications

(3 citation statements)

References 185 publications

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“…According to the findings, AgNPs are a good material for use as antibacterial agents against pathogenic bacterial species as also evident by previous research [56][57][58] . Recent findings indicate that silver and copper nanoparticles possess biocidal properties, making them suitable for use as antibacterial coatings on consumer goods 59 .…”

Section: E Inhibition Of Enzymatic Activitysupporting

confidence: 67%

Biosynthesis and assessment of antibacterial and antioxidant activities of silver nanoparticles utilizing Cassia occidentalis L. seed

Arya,

Tyagi,

Bhatnagar

et al. 2024

Sci Rep

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This research explores the eco-friendly synthesis of silver nanoparticles (AgNPs) using Cassia occidentalis L. seed extract. Various analytical techniques, including UV–visible spectroscopy, transmission electron microscopy (TEM), scanning electron microscopy (SEM), X-ray diffraction (XRD), and energy dispersive X-ray spectroscopy (EDX), were employed for comprehensive characterization. The UV–visible spectra revealed a distinct peak at 425 nm, while the seed extract exhibited peaks at 220 and 248 nm, indicating the presence of polyphenols and phytochemicals. High-resolution TEM unveiled spherical and oval-shaped AgNPs with diameters ranging from 6.44 to 28.50 nm. The SEM exhibiting a spherical shape and a polydisperse nature, thus providing insights into the morphology of the AgNPs. EDX analysis confirmed the presence of silver atoms at 10.01% in the sample. XRD results unequivocally confirm the crystalline nature of the AgNPs suspension, thereby providing valuable insights into their structural characteristics and purity. The antioxidant properties of AgNPs, C. occidentalis seed extract, and butylated hydroxytoluene (BHT) were assessed, revealing IC50 values of 345, 500, and 434 μg/mL, respectively. Antibacterial evaluation against Bacillus subtilis, Staphylococcus aureus, and Escherichia coli demonstrated heightened sensitivity of bacteria to AgNPs compared to AgNO3. Standard antibiotics, tetracycline, and ciprofloxacin, acting as positive controls, exhibited substantial antibacterial efficacy. The green-synthesized AgNPs displayed potent antibacterial activity, suggesting their potential as a viable alternative to conventional antibiotics for combating pathogenic bacterial infections. Furthermore, potential biomedical applications of AgNPs were thoroughly discussed.

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Section: E Inhibition Of Enzymatic Activitysupporting

confidence: 67%

Biosynthesis and assessment of antibacterial and antioxidant activities of silver nanoparticles utilizing Cassia occidentalis L. seed

Arya,

Tyagi,

Bhatnagar

et al. 2024

Sci Rep

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show abstract

“…Sj-AuNps significantly inhibited this LPS-induced nuclear translocation. Green synthesis nanotechnology provides a successful option for synthesizing new gold nanoparticles and suggests innovative new solutions for diseases [44,45]. Furthermore, the anti-inflammatory effects of Sj-AuNps are related to the suppression of the iNOS and COX-2 genes during the treatment in the form of reduction of the production of nitrites and PGE 2 .…”

Section: In Vitro Applications Of Sj-aunpsmentioning

confidence: 99%

Gold Nanoparticles Green-Synthesized by the Suaeda japonica Leaf Extract and Screening of Anti-Inflammatory Activities on RAW 267.4 Macrophages

et al. 2022

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Biosynthesis of gold nanoparticles from medicinal plants has become a modern strategy in biomedical research based on their exclusive properties, including specific targeting, lower toxicity, and biocompatibility. In this study, gold nanoparticles, reduced by the Suaeda japonica leaf extract, were promptly validated by UV–visible (UV–Vis) spectroscopy at 548 nm. No additional reducing agents were needed in this kind of a reduction reaction, which provided evidence of green synthesis. Dynamic light scattering (DLS), energy-dispersive X-ray spectroscopy (EDX), field-emission transmission electron microscopy (FE-TEM), selected area electron diffraction (SAED), and X-ray diffraction (XRD) analyses were used to illustrate the nanoscale characterization of S. japonica gold nanoparticles (Sj-AuNps). Furthermore, the cytotoxicity effect of Sj-AuNps against the RAW 264.7 cell line was determined by performing an MTT assay. We also investigated Sj-AuNps’ anti-inflammatory properties in LPS-induced murine macrophages. These nanoparticles reduced the generation of nitric oxide (NO) and prostaglandin E2 (PGE2) and repressed the expression of the LPS-stimulated inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) genes. This study presents a significant biomedical application of S. japonica AuNps. The anti-inflammatory capabilities of Sj-AuNps underline their potential as possible options for suppressing inflammation-mediated diseases.

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“…It is recognized that materials at the nanoscale possess unique optical, magnetic, catalytic, and electronic properties that differ from those of their bulk form [ 1 , 2 , 3 , 4 ]. These properties can be exploited to tackle a host of industrial, medical, and research problems.…”

Section: Introductionmentioning

confidence: 99%

Adaptation of a Bacterial Bioluminescent Assay to Monitor Bioeffects of Gold Nanoparticles

et al. 2022

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Our current study aimed to adapt a bioluminescent bacteria-based bioassay to monitor the bioeffects of gold nanoparticles (AuNPs). Luminous marine bacteria Photobacterium phosphoreum and AuNPs modified with polyvinylpyrrolidone were employed; low-concentration (≤10−3 g/L) bioeffects of AuNPs were studied. Bioluminescence intensity was used as an indicator of physiological activity in bacteria. Two additional methods were used: reactive oxygen species (ROS) content was estimated with a chemiluminescent luminol method, and bacterial size was monitored using electron microscopy. The bacterial bioluminescent response to AuNPs corresponded to the “hormesis” model and involved time-dependent bioluminescence activation, as well as a pronounced increase in the number of enlarged bacteria. We found negative correlations between the time courses of bioluminescence and the ROS content in bacterial suspensions, demonstrating the relationship between bioluminescence activation and bacterial ROS consumption. The combined effects of AuNPs and a beta-emitting radionuclide, tritium, revealed suppression of bacterial bioluminescent activity (as compared to their individual effects) and a reduced percentage of enlarged bacteria. Therefore, we demonstrated that our bacteria-based bioluminescence assay is an appropriate tool to study the bioeffects of AuNPs; the bioeffects can be further classified within a unified framework for rapid bioassessment.

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Gold Nanoparticle-Mediated Delivery of Therapeutic Enzymes for Biomedical Applications

Cited by 5 publications

References 185 publications

Biosynthesis and assessment of antibacterial and antioxidant activities of silver nanoparticles utilizing Cassia occidentalis L. seed

Biosynthesis and assessment of antibacterial and antioxidant activities of silver nanoparticles utilizing Cassia occidentalis L. seed

Gold Nanoparticles Green-Synthesized by the Suaeda japonica Leaf Extract and Screening of Anti-Inflammatory Activities on RAW 267.4 Macrophages

Adaptation of a Bacterial Bioluminescent Assay to Monitor Bioeffects of Gold Nanoparticles

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