Gold nanoparticles and a glucose oxidase based biosensor: an attempt to follow-up aging by XPS

Thibault, Sylvain; Aubriet, Hugues; Arnoult, Claire; Ruch, David

doi:10.1007/s00604-008-0028-z

Cited by 32 publications

(8 citation statements)

References 15 publications

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“…A tentative explanation of the observed loss of enzyme activity is based on the O 2 reduction with the subsequent production of H 2 O 2 . It is in fact reported [56][57][58] that H 2 O 2 can deactivate GOx by reacting with amino acid functions and hence leading in the loss of enzyme activity. 57…”

Section: Electrochemical Response To Glucose On Pt/gox-pva By CVmentioning

confidence: 99%

Mediator-free amperometric glucose biosensor based on glucose oxidase entrapped in poly(vinyl alcohol) matrix

Guascito

Chirizzi

Malitesta

et al. 2011

Analyst

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A simple and novel amperometric biosensor for glucose detection is proposed. It is based on the immobilization of glucose oxidase (GOx) in a poly(vinyl alcohol) (PVA) matrix directly drop casted on a platinum electrode surface (Pt/GOx-PVA). Glucose was determined in the absence of a mediator used to transfer electrons between the electrode and the enzyme. The correlation between peak current (i(p)) and scan rate has been verified and the effect of pH solution has been checked. Glucose detection has been performed amperometrically at -400 mV by using pulsed amperometric detection (PAD). Under the selected optimal conditions, the biosensor showed low detection limit (10 μM), wide dynamic range (0.1-37 mM) and high sensitivity. The biosensor amperometric response revealed it to be specific to glucose without significant interference from other sugars and electroactive species coexisting with glucose in biological fluids. Response stability was another interesting feature of the developed system as it was almost completely recovered when the biosensor was left in opportune storage conditions (i.e., a response decrease of only 13% after 35 days in air at room temperature). Finally, X-Ray Photoelectron Spectroscopy (XPS) characterization revealed a homogeneous film deposited on the Pt substrate whose structure is also preserved under operative conditions.

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Section: Electrochemical Response To Glucose On Pt/gox-pva By CVmentioning

confidence: 99%

Mediator-free amperometric glucose biosensor based on glucose oxidase entrapped in poly(vinyl alcohol) matrix

Guascito

Chirizzi

Malitesta

et al. 2011

Analyst

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“…[1][2][3][4]. Moreover, they have been extensively used in various application-oriented research areas ranging from electronic, memory and optical devices, [5][6][7][8] over biosensors, [8][9][10][11][12] biochips 13,14 and biomolecular logic gates 15 up to drug-delivery and targeting systems. 16,17 In addition, in the past decade, a number of molecular-and nanoelectronic devices (e.g., molecular transistors 18,19 and single-electron transistors 20,21 ) have been proposed utilizing single molecules and/or single nanoobjects (e.g., NPs, nanotubes, and nanogaps) and new physical phenomena in the nano-world (including quantum effects).…”

Section: Introductionmentioning

confidence: 99%

Gating capacitive field-effect sensors by the charge of nanoparticle/molecule hybrids

et al. 2015

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The semiconductor field-effect platform is a powerful tool for chemical and biological sensing with direct electrical readout. In this work, the field-effect capacitive electrolyte-insulator-semiconductor (EIS) structure - the simplest field-effect (bio-)chemical sensor - modified with citrate-capped gold nanoparticles (AuNPs) has been applied for a label-free electrostatic detection of charged molecules by their intrinsic molecular charge. The EIS sensor detects the charge changes in AuNP/molecule inorganic/organic hybrids induced by the molecular adsorption or binding events. The feasibility of the proposed detection scheme has been exemplarily demonstrated by realizing capacitive EIS sensors consisting of an Al-p-Si-SiO2-silane-AuNP structure for the label-free detection of positively charged cytochrome c and poly-d-lysine molecules as well as for monitoring the layer-by-layer formation of polyelectrolyte multilayers of poly(allylamine hydrochloride)/poly(sodium 4-styrene sulfonate), representing typical model examples of detecting small proteins and macromolecules and the consecutive adsorption of positively/negatively charged polyelectrolytes, respectively. For comparison, EIS sensors without AuNPs have been investigated, too. The adsorption of molecules on the surface of AuNPs has been verified via the X-ray photoelectron spectroscopy method. In addition, a theoretical model of the functioning of the capacitive field-effect EIS sensor functionalized with AuNP/charged-molecule hybrids has been discussed.

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“…A challenge with the current strategy of CEC generation lies in overcoming the distance that exists between the CNT framework and the redox centre of GOx in order to allow electron siphoning without disturbing the integrity of the molecule and facilitate electron transfer closer to the potential of the enzyme [68]. As with many redox proteins, the FAD-containing catalytic redox centre in native GOx is deeply embedded within the monomer with the only access being through a 1 nm 2 opening [26, 69], thereby removing it from direct communication with an electrode.…”

Section: Resultsmentioning

confidence: 99%

Cross-linked glucose oxidase clusters for biofuel cell anode catalysts

Dudzik¹,

Chang²,

Kannan³

et al. 2013

Biofabrication

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The efficient localization of increased levels of active enzymes onto conducting scaffolds is important for the development of enzyme-based biofuel cells. Crosslinked enzyme clusters (CEC) of glucose oxidase (GOx) constrained to functionalized carbon nanotubes (CEC-CNTs) were generated in order to evaluate the potential of using CECs for developing GOx-based bioanodes functioning via direct electron transfer from the GOx active site to the CNT scaffold. CEC-CNTs generated from several weight-to-weight ratios of GOx:CNT were examined for comparable catalytic activity to free GOx in solution, with CEC-CNTs generated from a 100% GOx solution displaying the greatest enzymatic activity. STEM analysis of CEC-CNTs generated from 100% GOx to CNT (wt/wt) ratios revealed CEC clusters of ~78 μm2 localized that the CNT surface. Electrochemical analysis indicates that the enzyme is engaging in direct electron transfer, and BFCs generated using GOx CEC-CNT bioanodes were observed to have a peak power density of ~180 μW·cm−2. These data indicate that the generation of nano-to-micro sized active enzyme clusters is an attractive option for the design of enzyme-specific biofuel cell powered implantable devices.

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Gold nanoparticles and a glucose oxidase based biosensor: an attempt to follow-up aging by XPS

Cited by 32 publications

References 15 publications

Mediator-free amperometric glucose biosensor based on glucose oxidase entrapped in poly(vinyl alcohol) matrix

Mediator-free amperometric glucose biosensor based on glucose oxidase entrapped in poly(vinyl alcohol) matrix

Gating capacitive field-effect sensors by the charge of nanoparticle/molecule hybrids

Cross-linked glucose oxidase clusters for biofuel cell anode catalysts

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