Gold nanoparticles as an adjuvant: Influence of size, shape, and technique of combination with CpG on antibody production

Дыкман, Л. А.; Староверов, С. А.; Фомин, А. С.; Khanadeev, Vitaly A.; Khlebtsov, Boris N.; Богатырев, В. А.

doi:10.1016/j.intimp.2017.11.008

Cited by 70 publications

(48 citation statements)

References 38 publications

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“…Isolated AuNPs with a typical size of 16 nm would also better penetrate the tumor tissue and have a chance to be degraded faster in healthy tissues within intracellular endolysosomes. 35 Although this study focused on bare citrate AuNP, when it comes to therapeutics or diagnosis, nanoparticle-functionalization is the key to ensuring tissue selectivity, e.g., tumor antigen-specific antibody 71 and aptamers, 29 thus ensuring that the nanoparticles selectively target the tissue of interest. Also, the use of a rather focused laser facilitates the irradiation of the desired tissue area, avoiding damage to healthy tissue.…”

Section: Discussionmentioning

confidence: 99%

Endocytosis-driven gold nanoparticle fractal rearrangement in cells and its influence on photothermal conversion

et al. 2020

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Section: Discussionmentioning

confidence: 99%

Endocytosis-driven gold nanoparticle fractal rearrangement in cells and its influence on photothermal conversion

et al. 2020

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“…Both species belong to the group of "rapidly growing Mycobacteria", in contrast to M. bovis, which belongs to "slowly growing Mycobacteria" [103]. A serological cross-reaction between the M. bovis and M. smegmatis cells (in contrast to M. phlei cells) was previously noted for antibodies obtained against a tuberculin-GNPs conjugate [96].…”

Section: Discussionmentioning

confidence: 85%

“…Size and shape of the GNPs (spheres, nanorods, nanoshells and nanostars) were shown to influence the immunogenicity of conjugates with haptens. Large spherical GNPs (d = 15 and 50 nm) are the optimal antigen carriers and adjuvants for immunization [96].…”

Section: Glyco-gnpsmentioning

confidence: 99%

Preparation and in vivo evaluation of glyco-gold nanoparticles carrying synthetic mycobacterial hexaarabinofuranoside

Burygin¹,

Abronina²,

Podvalnyy³

et al. 2020

Beilstein J. Nanotechnol.

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A number of bacterial glycans are specific markers for the detection and the serological identification of microorganisms and are also widely used as antigenic components of vaccines. The use of gold nanoparticles as carriers for glyco-epitopes is becoming an important alternative to the traditional conjugation with proteins and synthetic polymers. In this study, we aimed to prepare and evaluate in vivo glyco-gold nanoparticles (glyco-GNPs) bearing the terminal-branched hexaarabinofuranoside fragment (Ara6) of arabinan domains of lipoarabinomannan and arabinogalactan, which are principal polysaccharides of the cell wall of Mycobacterium tuberculosis, the causative agent of tuberculosis. In particular, we were interested whether the antibodies generated against Ara6-GNPs would recognize the natural saccharides on the cell surface of different mycobacterial strains. Two synthetic Ara6 glycosides with amino-functionalized spacer aglycons differing in length and hydrophilicity were directly conjugated with spherical gold nanoparticles (d = 15 nm) to give two sets of glyco-GNPs, which were used for the immunization of rabbits. Dot assays revealed cross-reactions between the two obtained antisera with the hexaarabinofuranoside and the 2-aminoethyl aglycon used for the preparation of glyco-GNPs. Both antisera contained high titers of antibodies specific for Mycobacteria as shown by enzyme-linked immunosorbent assay using M. bovis and M. smegmatis cells as antigens while there was only a weak response to M. phlei cells and no interaction with E. coli cells. The results obtained suggest that glyco-GNPs are promising agents for the generation of anti-mycobacterial antibodies.

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“…It is known that the color of GNPs solutions dramatically depends on their size and shape as well as on the dielectric properties of the medium surrounding the GNPs, these features being useful in many (bio)analytical applications including monitoring their stability (as described below) [25,26,46]. The choice of GNPs size and shape (spheres, nanorods, nanoshells and nanostars) were shown to influence immunogenicity of their conjugates with haptens, the large spherical GNPs (d = 15 and 50 nm) being the optimal antigen carriers and adjuvants for immunization [96]. NaCl from glyco-GNPs 3 or 4, which were purified by centrifugation, dissolved in 5% sucrose solution and then lyophilized.…”

Section: Glyco-gnpsmentioning

confidence: 99%

Preparation of glyco-gold nanoparticles carrying synthetic mycobacterial hexaarabinofuranoside and their evaluation in vivo

Burygin¹,

Abronina²,

Podvalnyy³

et al. 2019

Preprint

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Some bacterial glycans are specific markers for detection and serological identification of microorganisms and are widely used as antigenic components of vaccines. The use of gold nanoparticles as carriers for glyco-epitopes is becoming an important alternative to the traditional conjugation with proteins and synthetic polymers. In this study, we aimed at preparation and evaluation in vivo of glyco-gold nanoparticles (glyco-GNPs) bearing the terminal branched hexaarabinofuranoside fragment (Ara6) of arabinan domains of lipoarabinomannan and arabinogalactan, which are principal polysaccharides of the cell wall of Mycobacterium tuberculosis, the causative agent of tuberculosis. Specifically, we were interested in checking if the antibodies generated against Ara6-GNPs would recognize the natural saccharides on the cell surface of different mycobacterial strains. Two synthetic Ara6 glycosides with amino-functionalized spacer aglycons differing in length and hydrophilicity were directly conjugated with spherical gold nanoparticles (d = 15 nm) to give two sets of glyco-GNPs which were used for immunization of rabbits. Dot assay revealed cross-reactions for the two obtained antisera with the hexaarabinofuranoside with 2-aminoethyl aglycon used for the preparation of glyco-GNPs. Both antisera contained high titers of antibodies specific for Mycobacteria as shown by enzyme-linked immunosorbent assay using M. bovis and M. smegmatis cells as antigens while gave a weak response with M. phlei cells and no interaction with E. coli cells. The results obtained suggest that glyco-GNPs are promising agents for generation of anti-mycobacterial antibodies.

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Gold nanoparticles as an adjuvant: Influence of size, shape, and technique of combination with CpG on antibody production

Cited by 70 publications

References 38 publications

Endocytosis-driven gold nanoparticle fractal rearrangement in cells and its influence on photothermal conversion

Endocytosis-driven gold nanoparticle fractal rearrangement in cells and its influence on photothermal conversion

Preparation and in vivo evaluation of glyco-gold nanoparticles carrying synthetic mycobacterial hexaarabinofuranoside

Preparation of glyco-gold nanoparticles carrying synthetic mycobacterial hexaarabinofuranoside and their evaluation in vivo

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