Gold Nanoparticles Coated with Oligomannosides of HIV-1 Glycoprotein gp120 Mimic the Carbohydrate Epitope of Antibody 2G12

Marradi, Marco; Gianvincenzo, Paolo Di; Enríquez-Navas, Pedro M.; Martinez-Avila, Olga; Chiodo, Fabrizio; Yuste, Eloísa; Angulo, Jesús; Penadés, Soledad

doi:10.1016/j.jmb.2011.03.042

Cited by 73 publications

(55 citation statements)

References 57 publications

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“…Indeed, these AuNCs are excellent for quantitative studies of nanoparticle-protein interactions. However, only little experimental work has been performed [32,33]. In our study, the morphologies and microstructures of the synthetic BSAAuNCs were observed by TEM.…”

Section: Resultsmentioning

confidence: 91%

A sensitive electrochemical impedance immunosensor for determination of malachite green and leucomalachite green in the aqueous environment

Zhu

Pang

et al. 2016

Anal Bioanal Chem

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Application of malachite green (MG) and leucomalachite green (LMG) in fish farm water causes an environmental problem. This study proposes for the first time a sensitive and convenient electrochemical impedance spectroscopy (EIS) method for determining MG and LMG by a bovine serum albumin-decorated gold nanocluster (BSA-AuNC)/antibody composite film-based immunosensor. In order to improve the analytical performance, the glassy carbon electrode (GCE) was modified with 1, 4-phenylenediamine to form a stable layer, and then, BSA-AuNCs were covalently bound to the GCE. An adequate quantity of the polyclonal antibody of LMG was immobilized onto the surface of the BSA-AuNCs by the chemical reaction of EDC/NHS. The sensors can respond to the specific target based on specific covalent bonding. The experimental parameters, such as the pH, incubating concentration, and time, have been investigated and optimized. The calibration curve for LMG was linear in the range of 0.1~10.0 ng/mL with the limit of detection (LOD) 0.03 ng/mL. Furthermore, the sum of MG and LMG was detected in fish farm water by MG reduction. The recovery was between 89.7 % and 99.2 % in spiked samples. The EC sensor method was also compared with the ELISA method and validated by the LC-MS/MS method, which proves its great promise as a field instrument for the rapid monitoring of MG and LMG pollution. Graphical abstract 1, 4-Phenylenediamine and BSA-AuNC/antibody-decorated glassy carbon electrodes have been used for the impedimetric detection of the sum of malachite green and leucomalachite green via specific immuno-binding.

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Section: Resultsmentioning

confidence: 91%

A sensitive electrochemical impedance immunosensor for determination of malachite green and leucomalachite green in the aqueous environment

Zhu

Pang

et al. 2016

Anal Bioanal Chem

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“…30 In that work, it was shown that 10% of tetramannoside Te on the gold surface (Te-10-GNP) was enough to obtain an efficient 2G12 binding. In contrast, GNPs incorporating 10% of the pentamannoside P (P-10-GNP) showed poor interaction with 2G12 at the tested concentrations.…”

Section: Resultsmentioning

confidence: 98%

“…In addition, the GNPs coated with the tetramannoside inhibited the 2G12-mediated neutralization of HIV-1 in the micromolar range in cellular experiments. 30 Based on these results, we have now selected the tetramannoside Te as the main component of novel GNPs to generate better ligands for 2G12 towards a carbohydrate-based vaccine candidate against HIV. Herein, we describe the assembly of the three antennas of Man 9 GlcNAc 2 -D1 arm (tetramannoside, Te), D2 and D3 arms (pentamannoside, P)-on a gold surface to obtain a glyconanoparticle (Te/P-GNP) that re-builds the complete Man 9 in a multivalent fashion for better mimicking the viral carbohydrate envelope (Fig.…”

Section: Introductionmentioning

confidence: 99%

Assembling different antennas of the gp120 high mannose-type glycans on gold nanoparticles provides superior binding to the anti-HIV antibody 2G12 than the individual antennas

Chiodo

Enríquez-Navas

Angulo

et al. 2015

Carbohydrate Research

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“…In particular, linear trimannosides and tetramannosides bind to 2G12 [32] and multivalent gold nanoparticles coated with selfassembled monolayers of synthetic oligomannosides inhibited the binding between 2G12 and gp120 with IC 50 values in the micromolar range [33].…”

Section: Next-generation Hiv-1 Immunogens For Eliciting Bnab Responsesmentioning

confidence: 99%

Vaccine Nanoparticles for Protection Against HIV Infection

Aikins

Bazzill

Moon

2017

Nanomedicine (Lond.)

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The development of a successful vaccine against HIV is a major global challenge. Antiretroviral therapy is the standard treatment against HIV-1 infection. However, only 46% of the eligible people received the therapy in 2015. Furthermore, suboptimal adherence poses additional obstacles. Therefore, there is an urgent need for an HIV-1 vaccine. The most promising clinical trial to date is Phase III RV144, which for the first time demonstrated the feasibility of vaccine-mediated immune protection against HIV-1. Nevertheless, its 31% efficacy and limited durability underscore major hurdles. Here, we discuss recent progress in HIV-1 vaccine development with a special emphasis on nanovaccines, which are at the forefront of efforts to develop a successful HIV-1 vaccine.

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Gold Nanoparticles Coated with Oligomannosides of HIV-1 Glycoprotein gp120 Mimic the Carbohydrate Epitope of Antibody 2G12

Cited by 73 publications

References 57 publications

A sensitive electrochemical impedance immunosensor for determination of malachite green and leucomalachite green in the aqueous environment

A sensitive electrochemical impedance immunosensor for determination of malachite green and leucomalachite green in the aqueous environment

Assembling different antennas of the gp120 high mannose-type glycans on gold nanoparticles provides superior binding to the anti-HIV antibody 2G12 than the individual antennas

Vaccine Nanoparticles for Protection Against HIV Infection

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