Gold nanoparticles decorated with ovalbumin-derived epitopes: effect of shape and size on T-cell immune responses

Egorova, E. A.; Lamers, Gerda E. M.; Monikh, Fazel Abdolahpur; Boyle, Aimee L.; Slütter, Bram; Kros, Alexander

doi:10.1039/d2ra03027f

Cited by 5 publications

(6 citation statements)

References 53 publications

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“…It is a question if part of the AuNP carriers escaped the endosomes and delivered antimir-135b into the cytoplasm or if the short RNA was released from the carrier and transported to the cytoplasm during the endosome maturation endolysosomes. The physicochemical properties of nanocarriers, such as size, shape, surface charge, and surface coating, as well as the amounts of individual molecules, are important for their fate in cells. , For example, clathrin-independent endocytosis was observed in the case of folic acid, whereas clathrin-dependent and -independent endocytosis was observed in the case of folic acid-conjugated NPs . The nonbiological AuNPs were reported to be localized in the subcellular vesicles and rarely within the organelles or cytosol .…”

Section: Resultsmentioning

confidence: 99%

Bioproduced Nanoparticles Deliver Multiple Cargoes via Targeted Tumor Therapy In Vivo

Pourali,

Neuhöferová,

Dzmitruk

et al. 2024

ACS Omega

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This study recognized biologically produced gold nanoparticles (AuNPs) as multiple cargo carriers with a perspective of drug delivery into specialized tumor cells in vivo. Paclitaxel (PTX), transferrin, and antimiR-135b were conjugated with AuNPs and their uptake by mouse tumor cells in an induced breast cancer model was investigated. Each of the above-mentioned molecules was conjugated to the AuNPs separately as well as simultaneously, loading efficiency of each cargo was assessed, and performance of the final product (FP) was judged. After tumor induction in BALB/c mice, sub-IC 50 doses of FP as well as control AuNPs, PTX, and phosphate buffered saline were administered in vivo. Round AuNPs were prepared using Fusarium oxysporum and exhibited a size of 13 ± 1.3 nm and a zeta potential of −35.8 ± 1.3 mV. The cytotoxicity of individual conjugates and FP were tested by MTT assay in breast tumor cells 4T1 and nontumor fibroblasts NIH/3T3 cells. The conjugation of individual molecules with AuNPs was confirmed, and FP (size of 54 ± 14 nm and zeta potential of −31.9 ± 2.08 mV) showed higher 4T1-specific toxicity in vitro when compared to control conjugates. After in vivo application of the FP, transmission electron microscopy analyses proved the presence of AuNPs in the tumor cells. Hematoxylin and eosin staining of the tumor tissue revealed that the FP group exhibited the highest amounts of inflammatory, necrotic, and apoptotic cells in contrast to the control groups. Finally, qPCR results showed that FP could transfect and suppress miR-135b expression in vivo, confirming the tumor-targeting properties of FP. The capacity of biologically produced gold nanoparticles to conjugate with multiple decorative molecules while retaining their stability and effective intracellular uptake makes them a promising alternative strategy superior to current drug carriers.

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Section: Resultsmentioning

confidence: 99%

Bioproduced Nanoparticles Deliver Multiple Cargoes via Targeted Tumor Therapy In Vivo

Pourali,

Neuhöferová,

Dzmitruk

et al. 2024

ACS Omega

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“…In a different study, model MHC-I and MHC-II epitopes derived from chicken ovalbumin were conjugated to the gold NPs and nanorods, and the effect of shape and size on T-cell immune responses were analyzed. [35] A pro-drug NP was formulated by conjugating a bi-functional peptide-consisting of anti-PDÀ L1 peptide and cathepsin B-specific cleavable peptide-to a cancer chemotherapeutics, doxorubicin (DOX). [36] The resulting conjugate self-assembled into prodrug NP.…”

Section: Peptide-conjugatedmentioning

confidence: 99%

“…In vivo studies showed that the NP formulation containing mannose unit improved dendritic cell activation and enhanced antigen‐specific T cell responses, resulting in higher antitumor immunity in tumor‐bearing mice as compared to NP formulation without mannose unit. In a different study, model MHC‐I and MHC‐II epitopes derived from chicken ovalbumin were conjugated to the gold NPs and nanorods, and the effect of shape and size on T‐cell immune responses were analyzed [35] …”

Section: Peptide‐conjugated Nanoparticles For Drug Deliverymentioning

confidence: 99%

Peptide‐conjugated Nanoparticle Platforms for Targeted Delivery, Imaging, and Biosensing Applications

Ilieş,

Yildiz,

Abbas

2024

ChemBioChem

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Peptides have become an indispensable tool in engineering of multifunctional nanostructure platforms for biomedical applications such as targeted drug and gene delivery, imaging and biosensing. They can be covalently incorporated into a variety of nanoparticles (NPs) including polymers, metallic nanoparticles, and others. Using different bioconjugation techniques, multifunctional peptide‐modified NPs can be formulated to produce therapeutical and diagnostic platforms offering high specificity, lower toxicity, biocompatibility, and stimuli responsive behavior. Targeting peptides can direct the nanoparticles into specific tissues for targeted drug and gene delivery and imaging applications due to their specificity towards certain receptors. Furthermore, due to their stimuli‐responsive features, they can offer controlled release of therapeutics into desired sites of disease. In addition, peptide‐based biosensors and imaging agents can provide non‐invasive detection and monitoring of diseases including cancer, infectious diseases, and neurological disorders. In this review, we covered the design and formulation of recent peptide‐based NP platforms, as well as their utilization in in vitro and in vivo applications such as targeted drug and gene delivery, targeting, sensing, and imaging applications. In the end, we provided the future outlook to design new peptide conjugated nanomaterials for biomedical applications.

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“…It is a question if part of the AuNP carriers escaped the endosomes and delivered antimir-135b into cytoplasm or if the short RNA was released from the carrier and transported to cytoplasm during the endosome maturation endolysosomes. The physicochemical properties of nanocarriers, such as size, shape, surface charge and surface coating, as well as the amounts of individual molecules, are important for their fate in cells [44,45]. For example, clathrin-independent endocytosis was observed in the case of folic acid, whereas clathrin-dependent and -independent endocytosis was observed in the case of folic acid-conjugated NPs [45].…”

Section: Assessment Of Antimir-135b Activity and Reduction Of Oncogen...mentioning

confidence: 99%

“…For example, clathrin-independent endocytosis was observed in the case of folic acid, whereas clathrin-dependent and -independent endocytosis was observed in the case of folic acid-conjugated NPs [45]. The non-biological AuNPs were reported to be localized in the subcellular vesicles and rarely within the organelles or cytosol [44]. On the other hand, the biologically produced AuNPs with capping agent and multiple functionalization, the FPs, used in this study were observed within organelles and cytosol.…”

Section: Assessment Of Antimir-135b Activity and Reduction Of Oncogen...mentioning

confidence: 99%

Multicomponent delivery system based on biologically produced gold nanoparticles for targeted therapy of breast cancer in vivo

Pourali,

Neuhoferova,

Dzmitruk

et al. 2024

Preprint

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This study recognized biologically produced gold nanoparticles (AuNP) as multiple cargo carriers with a perspective of drugs delivery into specialized tumor cells in vivo. Paclitaxel (PTX), transferrin, and antimiR-135b were conjugated with AuNPs and their uptake by mouse tumor cells in an induced breast cancer model was investigated. Each of the above-mentioned molecules was conjugated to the AuNPs separately as well as simultaneously, loading efficiency of each cargo was assessed, and performance of the final product (FP) was assessed. After tumor induction in BALB/c mice, sub-IC50 doses of FP as well as control AuNPs, PTX, and PBS were administered in vivo. Round AuNPs were prepared using Fusarium oxysporum and exhibited size of 13 ± 1.3 nm and zeta potential of -35.8 ± 1.3 mV. The cytotoxicity of individual conjugates and FP were tested by MTT assay in breast tumor cells 4T1 and non-tumor fibroblasts NIH/3T3 cells. The conjugation of the individual molecules with AuNPs was confirmed and FP (size of 54 ± 14 nm and zeta potential of -31.9 ± 2.08 mV) showed higher 4T1-specific toxicity in vitro when compared to control conjugates. After in vivo application of the FP, TEM analyses proved presence of AuNPs in the tumor cells. Hematoxylin and eosin staining of the tumor tissue revealed that the FP group exhibited the highest amounts of inflammatory, necrotic, and apoptotic cells in contrast to control groups. Finally, qPCR results showed that FP could transfect and suppress miR-135b expression in vivo, confirming the tumor-targeting properties of FP. The capacity of biologically produced gold nanoparticles to conjugate with multiple decorative molecules while remaining their stability and effective intracellular uptake makes them promising alternative strategy superior to current drug carriers.

show abstract

Gold nanoparticles decorated with ovalbumin-derived epitopes: effect of shape and size on T-cell immune responses

Abstract: Gold nanoparticles (GNPs) can be manufactured in various shapes, and their size is programmable, which permits the study of the effects imposed by these parameters on biological processes.

Cited by 5 publications

References 53 publications

Bioproduced Nanoparticles Deliver Multiple Cargoes via Targeted Tumor Therapy In Vivo

Bioproduced Nanoparticles Deliver Multiple Cargoes via Targeted Tumor Therapy In Vivo

Peptide‐conjugated Nanoparticle Platforms for Targeted Delivery, Imaging, and Biosensing Applications

Multicomponent delivery system based on biologically produced gold nanoparticles for targeted therapy of breast cancer in vivo

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