Gold Nanoparticles Synthesis with Different Reducing Agents Characterized  by UV-Visible Spectroscopy and FTIR

Biz, Helena Mesquita; Moraes, Duane da Silva; Rocha, Tatiana Louise Ávila de Campos

doi:10.34178/jbth.v5i1.193

Cited by 1 publication

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“…AuNP can be manufactured via chemical and physical processes. The AuNP obtained from chemical methods is often conducted by using tetrachloroauric acid with sodium citrate, sodium borohydride or other toxic reagents as reducing and stabilizing agents for the synthesis process [ 12 , 13 ]. One study reported that the nanoparticles synthesized via green approaches demonstrated lower cytotoxicity than chemical methods [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

Therapeutic Applications of Mesenchymal Stem Cell Loaded with Gold Nanoparticles for Regenerative Medicine

et al. 2023

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In the present study, the various concentrations of AuNP (1.25, 2.5, 5, 10 ppm) were prepared to investigate the biocompatibility, biological performances and cell uptake efficiency via Wharton’s jelly mesenchymal stem cells and rat model. The pure AuNP, AuNP combined with Col (AuNP-Col) and FITC conjugated AuNP-Col (AuNP-Col-FITC) were characterized by Ultraviolet–visible spectroscopy (UV-Vis), Fourier-transform infrared spectroscopy (FTIR) and Dynamic Light Scattering (DLS) assays. For in vitro examinations, we explored whether the Wharton’s jelly MSCs had better viability, higher CXCR4 expression, greater migration distance and lower apoptotic-related proteins expression with AuNP 1.25 and 2.5 ppm treatments. Furthermore, we considered whether the treatments of 1.25 and 2.5 ppm AuNP could induce the CXCR4 knocked down Wharton’s jelly MSCs to express CXCR4 and reduce the expression level of apoptotic proteins. We also treated the Wharton’s jelly MSCs with AuNP-Col to investigate the intracellular uptake mechanisms. The evidence demonstrated the cells uptake AuNP-Col through clathrin-mediated endocytosis and the vacuolar-type H+-ATPase pathway with good stability inside the cells to avoid lysosomal degradation as well as better uptake efficiency. Additionally, the results from in vivo examinations elucidated the 2.5 ppm of AuNP attenuated foreign body responses and had better retention efficacy with tissue integrity in animal model. In conclusion, the evidence demonstrates that AuNP shows promise as a biosafe nanodrug delivery system for development of regenerative medicine coupled with Wharton’s jelly MSCs.

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Section: Introductionmentioning

confidence: 99%