Gold nanostars coated with neutral and charged polyethylene glycols: A comparative study of in-vitro biocompatibility and of their interaction with SH-SY5Y neuroblastoma cells

Pallavicini, Piersandro; Cabrini, Elisa; Cavallaro, Gennara; Chirico, Giuseppe; Collini, Maddalena; D’Alfonso, Laura; Dacarro, Giacomo; Donà, Alice; Marchesi, Nicoletta; Milanese, Chiara; Pascale, Alessia; Sironi, Laura; Taglietti, Angelo

doi:10.1016/j.jinorgbio.2015.05.002

Cited by 16 publications

(26 citation statements)

References 40 publications

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“…This conclusion agrees with previously reported negligible cellular uptake for similar systems with PEG-coated NRs. 17 It should be noted that the colloidal stability of PEG-coated GNPs and their weak interaction with negatively charged cells is a common property, which does not depend on a particular size and shape of GNPs. Based on obtained results, laser irradiation was carried out without removal of the particles from the medium to obtain the maximal amount of GNPs near the cell surface.…”

Section: Gold Nanoparticles Characterizationmentioning

confidence: 99%

Shape-dependent interaction of gold nanoparticles with cultured cells at laser exposure

Bibikova

Singh²,

Попов

et al. 2017

Laser Phys. Lett.

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Laser optoporation of cells by local heating of plasmonic gold nanoparticles (GNPs) was proven as a favorable delivery method of molecules into cells. The optoporation efficiency depends on the laser beam intensity and GNP properties. Here, we evaluate the membrane optoporation in vitro in terms of fluorescent dye permeability under treatment of a multi-pulsed nanosecond 1064-nm laser with a sharply-focused beam. Anisotropic GNPs, such as nanorods and nanostars, were fabricated to achieve the optimal GNP-cell interaction. Nanostars demonstrated highest optoporation efficacy with more than 80% of permeabilized cells within the illuminated area. By contrast to 2 common laser techniques, the laser beam scanning method results in cell optoporation within a controllable programmed in advance irradiated area.

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Section: Gold Nanoparticles Characterizationmentioning

confidence: 99%

Shape-dependent interaction of gold nanoparticles with cultured cells at laser exposure

Bibikova

Singh²,

Попов

et al. 2017

Laser Phys. Lett.

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“…GNS from LSB) that however present comparable dimensions and shape as the GNS from TX100 used in this paper. [24,25] In detail, using LSB instead of TX100 as the directing and protecting surfactant, leads to a mixture of monocrystalline and pentatwinned GNS, with the latter prevailing by far. [12,13] .…”

Section: Resultsmentioning

confidence: 99%

“…[15,24] Coating with thiolated polymers containing polyethylene glycol (HS-PEG) allowed complete surfactant removal, producing fully biocompatible GNS from LSB, as we have demonstrated on the SH-SY5Y cell line. [24,25] Coated GNS from LSB are not significantly internalized in cells, unless when overcoated with cationic polymers capable of membrane disruption, such as PAH (polyallylamine hydrochloride). [25] The size and shape of nanoparticles are parameters strongly affecting their internalization in cells.…”

Section: Introductionmentioning

confidence: 99%

“…[24,25] Coated GNS from LSB are not significantly internalized in cells, unless when overcoated with cationic polymers capable of membrane disruption, such as PAH (polyallylamine hydrochloride). [25] The size and shape of nanoparticles are parameters strongly affecting their internalization in cells. [26] The 40-50 nm range is typically considered optimal for cell internalization, although this has been shown for gold nanoparticles with a spherical shape.…”

Section: Introductionmentioning

confidence: 99%

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Synthesis of reduced-size gold nanostars and internalization in SH-SY5Y cells

Dacarro

Pallavicini

Bertani

et al. 2017

Journal of Colloid and Interface Science

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The synthesis of large pentatwinned five-branched gold nanostars (GNS) has been modified so to obtain overall dimensions shrunk to 60% and a lower branches aspect ratio, leading to a dramatic blue shift of their two near-infrared (NIR) localized surface plasmon resonances (LSPR) absorptions but still maintaining one LSPR in the biotransparent NIR range. The interactions of polyethylene glycol (PEG) coated large and shrunk GNS with SH-SY5Y cells revealed that the large ones (DCI - diameter of the circumference in which GNS can be inscribed=76nm) are internalized more efficiently than the shrunk ones (DCI=46nm), correlating with a decreased cells surviving fraction.

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“…Biocompatibility evaluation in terms of cytotoxicity was repeated three times for each one of three samples randomly selected from each of four groups (one group without surface treating and three groups after shot peening). Research was [32][33][34]. Neuroblastoma is the most common extra-cranial solid malignant tumour of the sympathetic nervous system in children.…”

Section: Methodsmentioning

confidence: 99%

Changes of cytotoxicity of Ti-6Al-4V alloy made by DMLS technology as effect of the shot peening

Żebrowski¹,

Walczak²,

Drozd³

et al. 2020

Ann Agric Environ Med.

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Objective. The aim the study was to investigate the impact of the shot peening process on the condition of the surface layer and biological properties of titanium alloy produced by means of the Direct Metal Laser Sintering (DMLS) method. Materials and method. Specimens were prepared by the EOSINT M280 metal sintering laser system. The surfaces were subjected to the shot peening process using three different media, i.e. steel shot, nutshell granules and ceramic beads, after which they were subjected to profilometric analysis, scanning electron microscopic (SEM) observations and energy dispersive X-ray spectroscopy (EDS) tests, as well as to assessment of biological compatibility in terms of cytotoxicity (SH-SY5Y cell lines). Results. The general results obtained from the tests indicate satisfactory biocompatibility of the examined surfaces and that the impact of the shot peening process on the titanium alloy cytotoxicity is acceptable. Conclusions. The lowest cytotoxicity was demonstrated by the surfaces modified by ceramic beads than the nutshells and the biggest steel shot correspondingly. Moreover, the shot peening process carried out by means of CrNi steel and ceramic shot caused the reduction of surface roughness when, for the surface processing by means of nutshell granules, the increase of surface roughness was observed compared with the unmodified surface of titanium alloy samples.

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Gold nanostars coated with neutral and charged polyethylene glycols: A comparative study of in-vitro biocompatibility and of their interaction with SH-SY5Y neuroblastoma cells

Cited by 16 publications

References 40 publications

Shape-dependent interaction of gold nanoparticles with cultured cells at laser exposure

Shape-dependent interaction of gold nanoparticles with cultured cells at laser exposure

Synthesis of reduced-size gold nanostars and internalization in SH-SY5Y cells

Changes of cytotoxicity of Ti-6Al-4V alloy made by DMLS technology as effect of the shot peening

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