Golden pompano (Trachinotus blochii) adapts to acute hypoxic stress by altering the preferred mode of energy metabolism

Sun, Jun; Liu, Yi Fan; Tian, Jiang; Li, Yan Qiang; Song, Fei; Wen, Xintian; Luo, Jian

doi:10.1016/j.aquaculture.2021.736842

Cited by 37 publications

(11 citation statements)

References 60 publications

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“…A previous study of ours described the mRNA profile from the brain and liver of golden pompano ( Sun et al, 2021 ). The results of RT-qPCR showed a significant positive correlation with the RNA-Seq results ( p < 0.05) ( Supplementary Figure S1 ), indicating that our sequencing results were accurate.…”

Section: Resultsmentioning

confidence: 97%

“…Our previous research results show that the brain and liver exhibit a synergistic response to promote adaptation to hypoxia, and that disorder of liver glucose and lipid metabolism under hypoxia stress may lead to the death of golden pompano ( Sun et al, 2021 ). Similar results were also found in this study using BSR-seq technology.…”

Section: Discussionmentioning

confidence: 99%

“…Although fish can usually cope with short-term hypoxia, there are still differences between individuals ( Sun et al, 2021 ). But it is difficult to accurately use these differences for hypoxia tolerance breeding.…”

Section: Introductionmentioning

confidence: 99%

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Identification of Candidate Genes Associated With Hypoxia Tolerance in Trachinotus blochii Using Bulked Segregant Analysis and RNA-Seq

Liu

Tian

Chen³

et al. 2021

Front. Genet.

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Golden Pompano (Trachinotus blochii) has rapidly developed into the one of the main valuable fish species in Chinese marine aquaculture. Due to its rapid growth, active metabolism, and high oxygen consumption, hypoxia will increase its mortality and cause serious economic losses. We constructed two experimental groups of fish with different degrees of tolerance to hypoxia, used BSR-Seq analysis based on genome and genetic linkage groups to locate SNPs and genes that were related to the differences in hypoxia tolerance. The results showed that hypoxia tolerance SNPs of golden pompano may be jointly determined by multiple linkage groups, especially linkage groups 18 and 22. There were 768 and 348 candidate genes located in the candidate regions of the brain and liver, respectively. These genes were mainly involved in anaerobic energy metabolism, stress response, immune response, waste discharge, and cell death. The prostaglandin-endoperoxide synthase 2 (PTGS2) on LG8, which is involved in the metabolism of arachidonic acid, has a G/A nonsynonymous mutation at position 20641628, and the encoded amino acid was changed from hydrophobic aspartic acid to asparaginate. The specific pathway of the RIG-I-like receptor signaling pathway in the liver may mediate the metabolic system and the immune system, linking glucose metabolism with immune regulation. The death of the hypoxia-intolerant group may be due to the accumulation of lactic acid caused by the activation of anaerobic glycolysis during the early stage of hypoxia stress, and the activation of type I interferon was inhibited, which resulted in decreased immunity. Among the genes involved in the RIG-I-like receptor signaling pathway, the CYLD Lysine 63 Deubiquitinase (CYLD) located on LG16 had a G/T nonsynonymous mutation at position 13629651, and the encoded amino acid was changed from alanine acid to valine. The interferon induced with helicase C domain 1 (Ifih1) located on LG18 has a G/C nonsynonymous mutation at position 16153700, and the encoded hydrophilic glycine was changed to hydrophobic alanine. Our findings suggest these SNPs may assist in the molecular breeding of hypoxia-tolerant golden pompano, and speculate that the balance of glucose and lipid metabolism plays a key role in Trachinotus blochii under acute hypoxia.

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Section: Resultsmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

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Identification of Candidate Genes Associated With Hypoxia Tolerance in Trachinotus blochii Using Bulked Segregant Analysis and RNA-Seq

Liu

Tian

Chen³

et al. 2021

Front. Genet.

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“…Besides lipid metabolism, glycolysis metabolism is another method of energy production for fish exposed to hypoxic conditions (Li et al, 2018; Sun et al, 2021). Lactate dehydrogenase (LDH) and PK have previously been shown to be under selection in fish exposed to environmental stress (Pierce & Crawford, 1997).…”

Section: Discussionmentioning

confidence: 99%

Biochemical, histological, and gene expression analysis in brain and heart in Phoxinus lagowskii under sustained and diel‐cycling hypoxia

Yao

Wang

et al. 2022

J World Aquaculture Soc

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Sustained and diel-cycling hypoxia frequently occurs in high-latitude environments; however, fish's biochemical, physiological, and histological responses to these two conditions remain unknown. Current hypotheses posit that the heart and the brain of fish from high latitudes are integral to their hypoxia tolerance and that fish exhibit different response mechanisms to cope with sustained and dielcycling hypoxic conditions. To test this, we acclimated Phoxinus lagowskii, a high-latitude fish, to ecologically relevant sustained and diel-cycling hypoxia conditions over 28 days. Histological analysis revealed changes in the relative thickness of the layers in the midbrain. There was a prominent upsurge of oxidative stress biomarkers in dielcycling hypoxia (DCH) treatments, with superoxide dismutase and catalase activity. Our data suggested that triglycerides in the heart were elevated in DCH groups but remained unchanged regardless of sustained hypoxia (SH) treatments. The majority of enzyme activities (LDH, PK, PFK, and HK) increased in DCH groups, especially at day 28. The gene expression results indicated that HIF mainly regulated long-term DCH in the heart and regulated SH in the brain. Collectively, these findings reveal that

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“…For channel catfish (Ictalurus punctatus), RNA-Seq analysis with swimbladder samples revealed that many genes affected by hypoxia were enriched in the HIF, MAPK, PI3K/Akt/mTOR, VEGF and Ras signaling pathways (Yang et al, 2018). Moreover, liver transcriptomes under hypoxia have been widely studied in aquaculture fishes such as large yellow croaker (Ding et al, 2020), silver sillago (Sillago sihama) (Tian et al, 2020a), largemouth bass (Micropterus salmoides) (Sun et al, 2020), golden pompano (Trachinotus blochii) (Sun et al, 2021b), rainbow trout (Oncorhynchus mykiss) (Hou et al, 2020) and Nile tilapia (Oreochromis niloticus) (Ma et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Acute hypoxia changes the gene expression profiles and alternative splicing landscape in gills of spotted sea bass (Lateolabrax maculatus)

et al. 2022

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Hypoxia is one of the most important environmental stressors in aquatic ecosystems. To deal with the hypoxia environment, fishes exhibit a series of physiological and molecular responses to maintain homeostasis and organism functions. In the present study, hypoxia-induced changes in gene expression profiles and alternative splicing (AS) events in spotted sea bass (Lateolabrax maculatus), a promising marine-culture fish species in China, were thoroughly investigated by RNA-Seq analysis. A total of 1,242, 1,487 and 1,762 differentially expressed genes (DEGs) were identified at 3 h, 6 h and 12 h in gills after hypoxia stress. Functional enrichment analysis by KEGG and GSEA demonstrated that HIF signal network system was significantly activated and cell cycle process was remarkably suppressed in response to hypoxia. According to the temporal gene expression profiles, six clusters were generated and protein-protein interaction (PPI) networks were constructed for the two clusters that enriched with hypoxia-induced (cluster 2) or -suppressed genes (cluster 5), respectively. Results showed that HIF signaling related genes including vegfa, igf1, edn1, cox2b, cxcr4b, ctnnb1, and slc2a1a, were recognized as hubs in cluster 2, while mcm2, chek1, pole, mcm5, pola1, and rfc4, that tightly related to cell cycle, were down-regulated and considered as hubs in cluster 5. Furthermore, a total of 410 differential alternative splicing (DAS) genes were identified after hypoxia, which were closely associated with spliceosome. Of them, 63 DAS genes also showed differentially expressed levels after hypoxia, suggesting that their expression changes might be regulated by AS mechanism. This study revealed the key biological pathways and AS events affected by hypoxia, which would help us to better understand the molecular mechanisms of hypoxia response in spotted sea bass and other fish species.

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Golden pompano (Trachinotus blochii) adapts to acute hypoxic stress by altering the preferred mode of energy metabolism

Cited by 37 publications

References 60 publications

Identification of Candidate Genes Associated With Hypoxia Tolerance in Trachinotus blochii Using Bulked Segregant Analysis and RNA-Seq

Identification of Candidate Genes Associated With Hypoxia Tolerance in Trachinotus blochii Using Bulked Segregant Analysis and RNA-Seq

Biochemical, histological, and gene expression analysis in brain and heart in Phoxinus lagowskii under sustained and diel‐cycling hypoxia

Acute hypoxia changes the gene expression profiles and alternative splicing landscape in gills of spotted sea bass (Lateolabrax maculatus)

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