GONAD DEVELOPMENT OF SANDFISH (Holothuria scabra) FED WITH VITAMIN E-SUPPLEMENTED DIET

Abstract

The experiment was conducted to evaluate the effect of vitamin E on the gonad development of sandfish, Holothuria scabra. Four experimental diets were formulated to contain different levels of vitamin E, i.e. 0, 150, 300, and 450 mg/kg diet. Dry diets were prepared at the same protein level (14%). The experiment was arranged in a completely randomized design with four treatments and three replicates. Before the experiment started, the internal organs of broodstock were removed (evisceration) by injecting KCl 1 N reagent into their body, and the gonads were observed to determine the sex of each broodstock. Mean total length and body weight of broodstock after the internal organ removal were 10.95 ± 0.37 cm and 61.12 ± 2.28 g, respectively. Three broodstock (one male and two females) were allocated into each plastic bucket with a volume of 150 L. Each plastic bucket was equipped with aeration and flow-through water system. Broodstock fed with experimental diet once daily at 3% of total biomass for 90 days. The results showed that vitamin E has significant effects on percentage of gonad maturation stages and gonad somatic index (GSI) (P<0.05). Vitamin E at 300 mg/kg diet was the best for gonad maturation of sandfish broodstock. Furthermore, 55.5% and 11.1% of broodstock fed a diet containing 300 mg vitamin E/kg diet reached gonad maturity stage-III and stage-IV, respectively, with GSI of 12.21%.

Influence of antioxidants on the growth performance, gonadosomatic index and biochemical properties of gonad and fertilization success in koi carp (Cyprinus carpioL.)

“…In the present study, both male and female koi carp exhibited higher growth performance indices only when fed with feed containing 200 mg/kg of vitamin E. The present result was in accordance with James et al (2008); Muchlisin et al (2016), and Sembiring and Hutapea (2019) who inferred that vitamin E at 300 mg/kg feed had the best weight gain and specific growth rate in goldfish ( Carassius auratus ), Javan mahseer ( Tor tambra ) and sand fish ( Holothuria scabra ) respectively. The authors also suggested that vitamin E affected the gonadal development, leading to gain average body weight in fish species.…”

“…It is also to be noted that the next highest GSI was found in koi carps fed with feed containing vitamin E at 600 mg/kg feed. In addition, studies reported by Sembiring and Hutapea (2019) in sand fish (Holothuria scabra) and Muslimin et al (2020) in climbing perch (Anabas testudineus) have mentioned that GSI was found to be improved after higher inclusion of vitamin E in diets. Therefore, it can be inferred that both vitamins C and E were able to produce higher GSI in males only at fish fed with diets containing higher inclusion rate of vitamins.…”