Gonadotropins and Estradiol Stimulate Immunoreactive Insulin-Like Growth Factor-I Production by Porcine Granulosa Cells in Vitro*

Hsu, Chih-Jung; Hammond, James M.

doi:10.1210/endo-120-1-198

Cited by 246 publications

(82 citation statements)

References 44 publications

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“…In both rat (Borland etal., 1984 ;Mita etal., 1985 ;Oonk etal., 1987a, b) and pig (Jaillard et al, 1987, present (Hammond et al, 1985;Hsu and Hammond, 1987) and rat (Davoren and Hsueh, 1986) granulosa cells and their purtative male homolog, Sertoli cells from the same species (Ritzen, 1983 ;Tres et al, 1986 ;Smith et al, 1987 ;IChatelain etal., 1987), secrete Sm-C/IGF-I immunoreactivity in the medium. The Sm-C/IGF-I-like peptide purified from rat (Smith etal., 1987) The physiological in vivo significance of the endocrine/paracrine/autocrine role of Sm-C/IGF-I on testicular growth and functions is suggested by the fact that in both humans (Tanner et al, 1975 ;Kulin et al, 1981 ;Laron, 1984) Les LC contiennent des récepteurs spécifiques pour la Sm-C/IGF-1 et pour l'insuline, tandis que les SC contiennent seulement des récepteurs pour la Sm-C/IGF-1.…”

mentioning

confidence: 99%

Differentiating effects of somatomedin-C/insulin-like growth factor I and insulin on Leydig and Sertoli cell functions

Saez¹,

Chatelain²,

Perrard-Sapori³

et al. 1988

Reprod. Nutr. Dévelop.

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mentioning

confidence: 99%

Differentiating effects of somatomedin-C/insulin-like growth factor I and insulin on Leydig and Sertoli cell functions

Saez¹,

Chatelain²,

Perrard-Sapori³

et al. 1988

Reprod. Nutr. Dévelop.

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“…Estradiol was also shown to increase IGF 1 in porcine granulosa cells (Hsu CJ, Hammond, 1987). But in the bovine granulosa cells, it was shown that treatment with anti IGF receptor antibody which effectively blocked the protective effect of IGF 1 against apoptosis, did not prevent the protective effect of estradiol (Quirck et al, 2004).…”

Section: Estradiolmentioning

confidence: 96%

“…EGF, TGFα, and bFGF, as well as their receptors, have been found in the ovary (Hsu and Hammond, 1987;Khan-Dawood, 1987;Kudlow et al, 1987) and shown to inhibit spontaneous onset of apoptotic DNA cleavage in cultured granulosa cells (Tilly et al, 1992a, b). EGF suppression of granulosa cell apoptosis is mediated by the stimulation of progesterone production and the regulation of intracellular free calcium concentration (Luciano et al, 1994).…”

Section: Growth Factorsmentioning

confidence: 99%

Role of apoptosis-related factors in follicular atresia

Gumus¹,

Kılıç²,

Zülfikaroğlu³

2013

Int. J. Genet. Mol. Biol.

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Follicular atresia is the term used for the fate of follicles which undergo degenerative changes before rupturing during ovulation. Recent studies suggest that granulosa cell apoptosis play a major role in follicular atresia. The factors which lead the cell to apoptosis and which protect the cell death, still remain complicated and more studies are needed to elucidate the whole process. Here in this review, we aimed to simplify the factors and mechanisms taking place in granulosa cell apoptosis, to make the process more understandable.Key words: Granulosa cell, apoptosis, follicular atresia. INTRODUCTİONWhen a female human embryo is 8-week-old, she has 600,000 germ cells in her gonads and this number increases to 6 to 7 million at 20 weeks of gestation. But with the increasing rate of atresia, the number declines progressively and 1 million oocytes are present in the newborn, whereas 300,000 oocytes remain in puberty, of which approximately 400 will ovulate during the fertile lifespan (Oktem and Oktay, 2008;Gougeon, 1996;Matova and Cooley, 2001).'Follicular atresia' term is used to define antral follicles undergoing degenerative changes before rupturing during ovulation. It is initiated within the granulosa cell layer and subsequently in the theca cells Hsueh et al., 1994). In mammals, the basic mechanism of follicular atresia is apoptosis (Depalo et al., 2003). Apoptosis is a way which multicellular organisms use to eliminate unwanted cells in response to developmental signals or toxic stimuli (Quirck et al., 2004). It is regulated at the level of transcription or translation (Manabe et al., 2008). Major morphological characteristics of apoptosis are the internucleosomal DNA fragmentation, cell shrinkage, plasma membrane blebbing, and the apoptotic body formation (Schwartzman and Cidlowski, 1993).Granulosa cells possess endogenous pathways to trigger apoptosis, that are inhibited in the presence of survi-*Corresponding author. E-mail: zebru33@gmail.com. Tel: +90 (312) 2158652. Fax: +90 (312) 3124931.val factors (Quirck et al., 2004). To date, many apoptosisrelated factors have been implicated in follicular atresia, including death ligands and receptors, Bcl-2 family proteins, Nodal, caspases, growth factors, gonadotropins, and calcium. In this report, we will overview these factors one by one. Proapoptotic regulators in the cell death receptorligand systemDeath receptors constitute a subgroup within the tumor necrosis factor (TNF) receptor family. They are located on the cell surface, anchored to the cell membrane, are trimerized and have cytoplasmic death domains (DDs) which are necessary to induce apoptosis (Park et al., 2005;Ashkenazi and Dixit, 1998;Wallach et al., 1999). Fas receptor, TNF receptor, and TNF related apoptosis inducing ligand receptors (TRAILr) are the members of TNF receptor family that are found to have roles in follicular atresia in mammalian ovaries (Park et al., 2005).In most of the cases, the cell death receptor-mediated apoptosis takes place as follows:1) The cell death ligand binds to...

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“…In (Adashi et al, 1991). Furthermore, these cells can produce specific IGF-binding proteins (IGFBPs) (Hsu and Hammond, 1987;Ramasharma and Li, 1987;Voutilainen and Miller, 1987;Mondschein et al, 1990;Adashi et al, 1991;Stanko et al, 1994). (Koistinen et al, 1990) and that these cells synthesize and secrete IGFBP-1 (Suikkari et al, 1989 Correspondence.…”

mentioning

confidence: 99%

“…Ovarian granulosa cells have been shown to produce insulin-like growth factors I or II (IGF-I, IGF-II) in a number of species including rats, pigs, cows and humans (Adashi et al, 1991). Furthermore, these cells can produce specific IGF-binding proteins (IGFBPs) (Hsu and Hammond, 1987;Ramasharma and Li, 1987; Voutilainen and Miller, 1987;Mondschein et al, 1990;Adashi et al, 1991;Stanko et al, 1994). IGFBP-3 has been purified from pig follicular fluid (Ui et al, 1989), while others found it was present in follicular fluid from both small and large follicles.…”

mentioning

confidence: 99%

Effects of insulin-like growth factors and binding protein 1 on bovine granulosa cell division in anchorage-independent culture

Lavranos¹,

O'Leary²,

Rodgers³

1996

Reproduction

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5). An increase in the number of cells in the presence of IGF-I was also confirmed by DNA measurement (P < 0.05, n = 5) and by western immunoblotting analyses of La antigen (n = 3). IGFBP-1 significantly inhibited cell division stimulated by IGF-I (P < 0.001) and IGF-II (P < 0.001), but not that stimulated by the analogue, LR3IGF-I, which has low affinity for IGFBPs. Other factors also known to affect IGF synthesis or effectiveness (FSH, oestradiol, growth hormone) did not appear to influence division of granulosa cells when cultured under anchorage-independent conditions, while dibutyryl cAMP significantly inhibited cell division (P < 0.01, n = 5). In (Adashi et al, 1991). Furthermore, these cells can produce specific IGF-binding proteins (IGFBPs) (Hsu and Hammond, 1987;Ramasharma and Li, 1987;Voutilainen and Miller, 1987;Mondschein et al, 1990;Adashi et al, 1991;Stanko et al, 1994). (Koistinen et al, 1990) and that these cells synthesize and secrete IGFBP-1 (Suikkari et al, 1989 Correspondence.Received 5 January 1996. mitotic index first increasing and then decreasing (Lussier et al, 1987 Materials and Methods Isolation of granulosa cellsThe procedure for the isolation of bovine granulosa cells and their subsequent culture under anchorage-independent con¬ ditions used in this study was a modification of the method described by (Fig. 1) (Fig. 1). IGF-I enhanced the rate above that of controls after 48 h of culture (Fig. 1) (Fig. 2) [3H]thymidine incorporation are shown (Fig. 3) (Francis et al, 1992), was examined. IGFBP-1 had no effect on the stimulatory effect of longR3IGF-I (108 ± 10% of the mean ± sem concentrations observed with longR3IGF-I alone, = 3), suggesting that the inhibitory effect of IGFBP-I on IGF-stimulated cell division was via its ability to bind IGF-I. In another set of experiments (Fig. 4), IGF-I increased the total content of DNA (per dish) but the effect was not significant.

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Gonadotropins and Estradiol Stimulate Immunoreactive Insulin-Like Growth Factor-I Production by Porcine Granulosa Cells in Vitro*

Cited by 246 publications

References 44 publications

Differentiating effects of somatomedin-C/insulin-like growth factor I and insulin on Leydig and Sertoli cell functions

Differentiating effects of somatomedin-C/insulin-like growth factor I and insulin on Leydig and Sertoli cell functions

Role of apoptosis-related factors in follicular atresia

Effects of insulin-like growth factors and binding protein 1 on bovine granulosa cell division in anchorage-independent culture

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